Transposable elements shape the evolution of mammalian development

Nature Reviews Genetics, Journal Year: 2021, Volume and Issue: 22(11), P. 691 - 711

Published: Aug. 5, 2021

Language: Английский

---

Human placental development and function

Seminars in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 131, P. 66 - 77

Published: April 4, 2022

The placenta is a transient fetal organ that plays critical role in the health and wellbeing of both fetus its mother. Functionally, sustains growth as it facilitates delivery oxygen nutrients removal waste products. Not surprisingly, defective early placental development primary cause common disorders pregnancy, including recurrent miscarriage, restriction, pre-eclampsia stillbirth. Adverse pregnancy conditions will also affect life-long via developmental programming[1]. Despite importance reproductive success health, our understanding not extensive, largely due to ethical limitations studying or chronological development, lack long-term vitro models, comparative animal models. In this review, we examine current knowledge human discuss maternal endometrium fetal-maternal dialogue success, explore latest models trophoblast endometrial stem cells. addition, formation function, how nutrient mediated during periods histotrophic nutrition (uptake uterine secretions) haemotrophic (exchange between circulations), endocrine function development.

Language: Английский

---

CCL2: An important cytokine in normal and pathological pregnancies: A review

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 13

Published: Jan. 6, 2023

C-C motif ligand 2 (CCL2), also known as monocytic chemotactic protein 1 (MCP-1), is an integral factor which recruits macrophages for the immune response. Together with its receptors (e.g., CCR2, ACKR1, and ACKR2), they exert noticeable influences on various diseases of different systems. At maternal-fetal interface, CCL2 detected to be expressed in trophoblasts, decidual tissue, myometrium, others. Meanwhile, existing reports have determined a series physiological regulators CCL2, functions maintaining normal recruitment immunocytes, tissue remodeling, angiogenesis. However, abnormal levels been reported associated adverse pregnancy outcomes such spontaneous abortion, preeclampsia preterm labor. In this review, we concentrate expression at well precise regulatory mechanisms classic signaling pathways, reveal multidimensional aspects pregnancy.

Language: Английский

---

DNA methylation in mammalian development and disease

Nature Reviews Genetics, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 12, 2024

The DNA methylation field has matured from a phase of discovery and genomic characterization to one seeking deeper functional understanding how this modification contributes development, ageing disease. In particular, the past decade seen many exciting mechanistic discoveries that have substantially expanded our appreciation for generic, evolutionarily ancient can be incorporated into robust epigenetic codes. Here, we summarize current distinct landscapes emerge over mammalian lifespan discuss they interact with other regulatory layers support diverse functions. We then review rising interest in alternative patterns found during senescence somatic transition cancer. Alongside advancements single-cell long-read sequencing technologies, collective insights made across these fields offer new opportunities connect biochemical genetic features cell physiology, developmental potential phenotype. Review, Smith et al. describe development within key disease states, as well different methyltransferases interface histone modifications proteins create maintain them.

Language: Английский

---

Single-nucleus multi-omic profiling of human placental syncytiotrophoblasts identifies cellular trajectories during pregnancy

Nature Genetics, Journal Year: 2024, Volume and Issue: 56(2), P. 294 - 305

Published: Jan. 24, 2024

Abstract The human placenta has a vital role in ensuring successful pregnancy. Despite the growing body of knowledge about its cellular compositions and functions, there been limited research on heterogeneity billions nuclei within syncytiotrophoblast (STB), multinucleated entity primarily responsible for placental function. Here we conducted integrated single-nucleus RNA sequencing ATAC analyses placentas from early late Our findings demonstrate dynamic developmental trajectories STB their correspondence with trophoblast stem cell (hTSC)-derived STB. Furthermore, identified transcription factors associated diverse nuclear lineages through gene regulatory networks experimentally confirmed function hTSC organoid-derived STBs. Together, our data provide insights into represent valuable resource interpreting pregnancy complications.

Language: Английский

---

Maternal circadian rhythms during pregnancy dictate metabolic plasticity in offspring

Cell Metabolism, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

---

The landscape of decidual immune cells at the maternal–fetal interface in parturition and preterm birth

Inflammation Research, Journal Year: 2025, Volume and Issue: 74(1)

Published: March 4, 2025

Parturition is similar to an inflammatory response in which resident and infiltrating immune cells release cytokines chemokines into the maternal-fetal interface, promoting expulsion of fetus from mother. The untimely activation these pathways can result preterm labor. interface composed mainly decidual tissue placental villous space. objective this review examine role mechanisms during parturition birth. A deeper understanding at could provide significant insight birth pathogenesis. We searched major databases (including PubMed, Web Science, Google Scholar etc.) for literature encompassing cells, up July 2024 combined with studies found reference lists included studies. Decidual neutrophils mediators that facilitate parturition. M1/M2 ratio macrophages increases among population. Mast may cause uterine contractions. In birth, there increase CD56dimCD16+ natural killer immature dendritic cells. Th1/Th2 Th17/Treg leads Women had a higher proportion B ILC2 help protect steady-state environment interface. invariant NKT plays important inflammation-induced These communicate each other. development sequencing technology enables more in-depth study dynamic balance microenvironment crucial maintaining human pregnancy initiation delivery. deep mechanism dysfunction pathogenesis

Language: Английский

---

Single-Cell RNA-seq Identifies Cell Subsets in Human Placenta That Highly Expresses Factors Driving Pathogenesis of SARS-CoV-2

Frontiers in Cell and Developmental Biology, Journal Year: 2020, Volume and Issue: 8

Published: Aug. 19, 2020

Infection by the Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) results in novel coronavirus disease COVID-19, which has posed a serious threat globally. of SARS-CoV-2 during pregnancy is associated with complications such as preterm labor and premature rupture membranes, proportion neonates born to infected mothers are also positive for virus. During pregnancy, placental barrier protects fetus from pathogens ensures healthy development. To predict if placenta permissive SARS-CoV-2, we utilized publicly available single-cell RNA-seq data identify cells express necessary factors required infection. binding receptor ACE2 S protein priming protease TMPRSS2 co-expressed subset syncytiotrophoblasts (STB) first trimester extravillous trophoblasts (EVT) second human placenta. In addition, non-canonical BSG/CD147 other proteases (CTSL, CTSB, FURIN) detected most cells. Other family receptors (ANPEP DPP4) were expressed Additionally, term multiple species including humans ACE2, DPP4, ANPEP along viral proteases. The ACE2- TMPRSS2-positive (ACE2 + +) subsets mRNA proteins involved budding replication. These had that physically interact host Further, discovered unique signatures genes STBs EVTs. highly differentiated involving mitochondrial metabolism glucose transport. EVTs enriched markers endovascular trophoblasts. Both these subtypes abundantly Toll-like pathway. components JAK-STAT pathway drives inflammation. We carried out systematic review identified 12% pregnant women was virus STBs. conclude, herein have uncovered cellular targets entry shown can potentially drive viremia developing Our provide basic framework toward understanding paraphernalia infections pregnancy.

Language: Английский

---

TEAD4 ensures postimplantation development by promoting trophoblast self-renewal: An implication in early human pregnancy loss

Proceedings of the National Academy of Sciences, Journal Year: 2020, Volume and Issue: 117(30), P. 17864 - 17875

Published: July 15, 2020

Significance Reproductive success in placental mammals relies on proper development of the trophoblast lineage. In particular, a balance self-renewal vs. differentiation progenitors is critical for establishment pregnancy. A defect this process causes early pregnancy loss. Here, we showed that Hippo signaling effector, TEAD4, essential progenitor postimplantation mammalian embryos. Using genetic mouse models and human TSCs, including TSCs from patients with recurrent losses, identified TEAD4-dependent, evolutionarily conserved gene expression program promotes stemness cell proliferation ensures utero survival developing fetus.

Language: Английский

---

Δ9-tetrahydrocannabinol exposure during rat pregnancy leads to symmetrical fetal growth restriction and labyrinth-specific vascular defects in the placenta

Scientific Reports, Journal Year: 2020, Volume and Issue: 10(1)

Published: Jan. 17, 2020

1 in 5 women report cannabis use during pregnancy, with nausea cited as their primary motivation. Studies show that (-)-△9-tetrahydrocannabinol (Δ9-THC), the major psychoactive ingredient cannabis, causes fetal growth restriction, though mechanisms are not well understood. Given critical role of placenta to transfer oxygen and nutrients from mother, fetus, any compromise development fetal-placental circulation significantly affects maternal-fetal exchange thereby, growth. The goal this study was examine, rats, impact maternal Δ9-THC exposure on development, neonatal outcomes, placental development. Dams received a daily intraperitoneal injection (i.p.) vehicle control or (3 mg/kg) embryonic (E)6.5 through 22. were allowed deliver normally measure pregnancy subset sacrificed at E19.5 for assessment via immunohistochemistry qPCR. Gestational resulted pups born symmetrical catch up by post-natal day (PND)21. During there no changes food intake, weight gain, litter size, gestational length. placentas Δ9-THC-exposed pregnancies exhibited phenotype characterized increased labyrinth area, reduced Epcam expression (marker trophoblast progenitors), altered blood space, decreased capillary area an recruitment pericytes greater collagen deposition, when compared controls. Further, had glucose transporter (GLUT1) glucocorticoid receptor (GR) response exposure. In conclusion, effectively compromised growth, which may be result adversely affected zone These findings implicate GLUT1 target provide potential mechanism restriction observed who pregnancy.

Language: Английский

---