Bone-organ axes: bidirectional crosstalk DOI Creative Commons

An-Fu Deng,

Fuxiao Wang, Sicheng Wang

et al.

Military Medical Research, Journal Year: 2024, Volume and Issue: 11(1)

Published: June 12, 2024

Abstract In addition to its recognized role in providing structural support, bone plays a crucial maintaining the functionality and balance of various organs by secreting specific cytokines (also known as osteokines). This reciprocal influence extends these modulating homeostasis development, although this aspect has yet be systematically reviewed. review aims elucidate bidirectional crosstalk, with particular focus on osteokines. Additionally, it presents unique compilation evidence highlighting critical function extracellular vesicles (EVs) within bone-organ axes for first time. Moreover, explores implications crosstalk designing implementing bone-on-chips assembloids, underscoring importance comprehending interactions advancing physiologically relevant vitro models. Consequently, establishes robust theoretical foundation preventing, diagnosing, treating diseases related axis from perspective cytokines, EVs, hormones, metabolites.

Language: Английский

Overcoming on-target, off-tumour toxicity of CAR T cell therapy for solid tumours DOI Open Access
Christian Flugel, Robbie G. Majzner, Giedre Krenciute

et al.

Nature Reviews Clinical Oncology, Journal Year: 2022, Volume and Issue: 20(1), P. 49 - 62

Published: Nov. 23, 2022

Language: Английский

Citations

249
Human disease models in drug development DOI Open Access

Anna Loewa,

James J. Feng, Sarah Hedtrich

et al.

Nature Reviews Bioengineering, Journal Year: 2023, Volume and Issue: 1(8), P. 545 - 559

Published: May 11, 2023

Language: Английский

Citations

142
Short chain fatty acids: key regulators of the local and systemic immune response in inflammatory diseases and infections DOI Creative Commons
Lisa-Marie Ney, Maximilian Wipplinger,

Martha Grossmann

et al.

Open Biology, Journal Year: 2023, Volume and Issue: 13(3)

Published: March 1, 2023

The human intestinal microbiome substantially affects health and resistance to infections in its dynamic composition varying release of microbial-derived metabolites. Short-chain fatty acids (SCFA) produced by commensal bacteria through fermentation indigestible fibres are considered key regulators orchestrating the host immune response microbial colonization regulating phagocytosis, chemokine central signalling pathways cell growth apoptosis, thereby shaping functionality epithelial barrier. Although research last decades provided valuable insight into pleiotropic functions SCFAs their capability maintain health, mechanistic details on how act across different types other organs not fully understood. In this review, we provide an overview various cellular metabolism, emphasizing orchestration along gut–brain, gut–lung gut–liver axes. We discuss potential pharmacological use inflammatory diseases highlight new options relevant three-dimensional organ models investigate validate biological more detail.

Language: Английский

Citations

105
Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip DOI Creative Commons
Gautam Mahajan,

Erin R. Doherty,

Tania To

et al.

Microbiome, Journal Year: 2022, Volume and Issue: 10(1)

Published: Nov. 26, 2022

Abstract Background A dominance of non-iners Lactobacillus species in the vaginal microbiome is optimal and strongly associated with gynecological obstetric health, while presence diverse obligate or facultative anaerobic bacteria a paucity species, similar to communities found bacterial vaginosis (BV), considered non-optimal adverse health outcomes. Various therapeutic strategies are being explored modulate composition microbiome; however, there no human model that faithfully reproduces epithelial microenvironment for preclinical validation potential therapeutics testing hypotheses about epithelium-microbiome interactions. Results Here, we describe an organ-on-a-chip (organ chip) microfluidic culture mucosa (vagina lined by hormone-sensitive, primary epithelium interfaced underlying stromal fibroblasts, which sustains low physiological oxygen concentration lumen. We show Vagina Chip can be used assess colonization L. crispatus consortia as well Gardnerella vaginalis- containing consortia, measure host innate immune responses. Co-culture growth on-chip was accompanied maintenance cell viability, accumulation D- L-lactic acid, physiologically relevant pH, down regulation proinflammatory cytokines. In contrast, co-culture G. vagina chip resulted injury, rise upregulation Conclusion This study demonstrates applying organ technology create better understand interactions between tissues, evaluate safety efficacy live biotherapeutics products.

Language: Английский

Citations

80
Exploring the promising potential of induced pluripotent stem cells in cancer research and therapy DOI Creative Commons
Matin Chehelgerdi,

Fereshteh Behdarvand Dehkordi,

Mohammad Chehelgerdi

et al.

Molecular Cancer, Journal Year: 2023, Volume and Issue: 22(1)

Published: Nov. 28, 2023

The advent of iPSCs has brought about a significant transformation in stem cell research, opening up promising avenues for advancing cancer treatment. formation is multifaceted process influenced by genetic, epigenetic, and environmental factors. offer distinctive platform investigating the origin cancer, paving way novel approaches to treatment, drug testing, tailored medical interventions. This review article will provide an overview science behind iPSCs, current limitations challenges iPSC-based therapy, ethical social implications, comparative analysis with other types also discuss applications tumorigenesis, future tumorigenesis highlight successful case studies utilizing research. conclusion summarize advancements made research importance continued investment iPSC unlock full potential these cells.

Language: Английский

Citations

71
Strategies to reduce the risks of mRNA drug and vaccine toxicity DOI
Dimitrios Bitounis, Eric Jacquinet, Maximillian A. Rogers

et al.

Nature Reviews Drug Discovery, Journal Year: 2024, Volume and Issue: 23(4), P. 281 - 300

Published: Jan. 23, 2024

Language: Английский

Citations

66
Poor Translatability of Biomedical Research Using Animals — A Narrative Review DOI Creative Commons
Lindsay J. Marshall, Jarrod Bailey, Manuela Cassotta

et al.

Alternatives to Laboratory Animals, Journal Year: 2023, Volume and Issue: 51(2), P. 102 - 135

Published: March 1, 2023

The failure rate for the translation of drugs from animal testing to human treatments remains at over 92%, where it has been past few decades. majority these failures are due unexpected toxicity — that is, safety issues revealed in trials were not apparent tests or lack efficacy. However, use more innovative tools, such as organs-on-chips, preclinical pipeline drug testing, tools able predict events prior clinical and so can be used this, well efficacy testing. Here, we review several disease areas, consider how models failed offer effective new treatments. We also make some suggestions human-relevant approach methodologies might applied address this.

Language: Английский

Citations

63
Organ-on-a-chip meets artificial intelligence in drug evaluation DOI Creative Commons
Shiwen Deng, Caifeng Li, Junxian Cao

et al.

Theranostics, Journal Year: 2023, Volume and Issue: 13(13), P. 4526 - 4558

Published: Jan. 1, 2023

Drug evaluation has always been an important area of research in the pharmaceutical industry. However, animal welfare protection and other shortcomings traditional drug development models pose obstacles challenges to evaluation. Organ-on-a-chip (OoC) technology, which simulates human organs on a chip physiological environment functionality, with high fidelity reproduction organ-level physiology or pathophysiology, exhibits great promise for innovating pipeline. Meanwhile, advancement artificial intelligence (AI) provides more improvements design data processing OoCs. Here, we review current progress that made generate OoC platforms, how single multi-OoCs have used applications, including testing, disease modeling, personalized medicine. Moreover, discuss issues facing field, such as large reproducibility, point integration OoCs AI analysis automation, is benefit future Finally, look forward opportunities faced by coupling AI. In summary, advancements development, combinations AI, will eventually break state

Language: Английский

Citations

56
Simulation-based approaches for drug delivery systems: Navigating advancements, opportunities, and challenges DOI Creative Commons

Iman Salahshoori,

Mahdi Golriz,

Marcos A.L. Nobre

et al.

Journal of Molecular Liquids, Journal Year: 2023, Volume and Issue: 395, P. 123888 - 123888

Published: Dec. 27, 2023

Efficient drug delivery systems (DDSs) play a pivotal role in ensuring pharmaceuticals' targeted and effective administration. However, the intricate interplay between formulations poses challenges their design optimization. Simulations have emerged as indispensable tools for comprehending these interactions enhancing DDS performance to address this complexity. This comprehensive review explores latest advancements simulation techniques provides detailed analysis. The encompasses various methodologies, including molecular dynamics (MD), Monte Carlo (MC), finite element analysis (FEA), computational fluid (CFD), density functional theory (DFT), machine learning (ML), dissipative particle (DPD). These are critically examined context of research. article presents illustrative case studies involving liposomal, polymer-based, nano-particulate, implantable DDSs, demonstrating influential simulations optimizing systems. Furthermore, addresses advantages limitations It also identifies future directions research development, such integrating multiple techniques, refining validating models greater accuracy, overcoming limitations, exploring applications personalized medicine innovative DDSs. employing like MD, MC, FEA, CFD, DFT, ML, DPD offer crucial insights into behaviour, aiding Despite advantages, rapid cost-effective screening, require validation addressing limitations. Future should focus on models, enhance outcomes. paper underscores contribution emphasizing providing valuable facilitating development optimization ultimately patient As we continue explore impact advancing discovery improving DDSs is expected be profound.

Language: Английский

Citations

56
Fecal microbiota transplantation: current challenges and future landscapes DOI
Abbas Yadegar, Haggai Bar‐Yoseph, Tanya Monaghan

et al.

Clinical Microbiology Reviews, Journal Year: 2024, Volume and Issue: 37(2)

Published: May 8, 2024

SUMMARYGiven the importance of gut microbial homeostasis in maintaining health, there has been considerable interest developing innovative therapeutic strategies for restoring microbiota. One such approach, fecal microbiota transplantation (FMT), is main "whole microbiome replacement" strategy and integrated into clinical practice guidelines treating recurrent

Language: Английский

Citations

49