Plasma metabolomic signatures for copy number variants and COVID-19 risk loci in Northern Finland populations DOI Creative Commons
Tisham De, Lachlan Coin, Jethro Herberg

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 16, 2025

Abstract Copy number variants (CNVs) are an important class of genomic variation known to be for human physiology and diseases. Here we present genome-wide metabolomic signatures CNVs in two Finnish cohorts—The Northern Finland Birth Cohort 1966 (NFBC 1966) NFBC 1986. We have analysed reported over 9,300 individuals characterised their dosage effect (CNV-metabolomic QTL) on 228 plasma lipoproteins metabolites. reference (normal physiology) up ~ 2.6 million COVID-19 GWAS results from the National Institutes Health (NIH) GRASP database, including outcomes related death, severity, hospitalisation. Furthermore, by analysing exemplar genes severity namely LZTFL1 OAS1, here additional candidate biology, (1) NFIX, a gene viral (adenovirus) replication hematopoietic stem cells (2) ACSL1, sepsis bacterial inflammation. Based our current literature hypothesise that charge imbalance across cellular membrane between cations (Fe 2+ , Mg etc.) anions (e.g. ROS, hydroxide ion Fenton reactions, superoxide etc.), iron trafficking within different cell types e.g., macrophages (3) systemic oxidative stress response lipid peroxidation mediated inflammation), together could relevance severe cases. To conclude, unique atlas univariate multivariate (~ 7.2 signatures) with deep annotations various multi-omics data sets provide knowledge base metabolism

Language: Английский

From Cell to Symptoms: The Role of SARS-CoV-2 Cytopathic Effects in the Pathogenesis of COVID-19 and Long COVID DOI Open Access
Pablo Gonzalez-Garcia, Ornella Fiorillo‐Moreno, Eloína Zarate Peñata

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(9), P. 8290 - 8290

Published: May 5, 2023

Severe Acute Respiratory Syndrome CoronaVirus 2 (SARS-CoV-2) infection triggers various events from molecular to tissue level, which in turn is given by the intrinsic characteristics of each patient. Given diversity characteristic cellular phenotype, possible cytopathic, and clinical effects are difficult predict, determines heterogeneity COVID-19 symptoms. The purpose this article provide a comprehensive review cytopathic SARS-CoV-2 on cell types, focusing development COVID-19, may lead, some patients, persistence symptoms after recovery disease, condition known as long COVID. We describe mechanisms underlying virus-host interactions, including alterations protein expression, intracellular signaling pathways, immune responses. In particular, highlights potential impact these cytopathies function outcomes, such dysregulation, neuropsychiatric disorders, organ damage. concludes discussing future directions for research implications management treatment

Language: Английский

Citations

16
HLA‐A*11:01 and HLA‐C*04:01 are associated with severe COVID‐19 DOI Open Access

Patricia Castro‐Santos,

Óscar Martínez-Nieto,

José A. Riancho

et al.

HLA, Journal Year: 2023, Volume and Issue: 102(6), P. 731 - 739

Published: Aug. 1, 2023

We analyzed the association between HLA polymorphisms and susceptibility to SARS‐CoV‐2 infection disease severity. Genotyping data from a total of 9373 COVID‐19‐positive cases Spanish Coalition Unlock Research on Host Genetics COVID‐19 (SCOURGE) consortium 5943 population controls were included in study. found an alleles HLA‐B*14:02 HLA‐C*08:02 with lower risk ( p = 0.006, OR 0.84, 95% CI [0.75–0.95], 0.024, 0.86, [0.78–0.95], respectively). also HLA‐A*11:01 HLA‐C*04:01 associated severity 0.033, 1.16, [1.04–1.31], 0.045, 1.14, [1.05–1.25], These results suggest that effective presentation viral peptides by class I involve faster clearance, decreasing COVID‐19.

Language: Английский

Citations

15
miRNAs as a Potential Biomarker in the COVID-19 Infection and Complications Course, Severity, and Outcome DOI Creative Commons
Milena Janković, Dejan Nikolić, Ivana Novaković

et al.

Diagnostics, Journal Year: 2023, Volume and Issue: 13(6), P. 1091 - 1091

Published: March 14, 2023

During the last three years, since emergence of COVID-19 pandemic, a significant number scientific publications have focused on resolving susceptibility to infection, as well course disease and potential long-term complications. is widely considered multisystem variety socioeconomic, medical, genetic/epigenetic factors may contribute severity outcome. Furthermore, SARS-COV-2 infection trigger pathological processes accelerate underlying conditions clinical entities. The development specific sensitive biomarkers that are easy obtain will allow for patient stratification, prevention, prognosis, more individualized treatments COVID-19. miRNAs proposed promising different aspects (susceptibility, severity, complication course, outcome, therapeutic possibilities). This review summarizes most relevant findings concerning miRNA involvement in pathology. Additionally, role wide range complications due accompanied and/or health discussed. importance understanding functional relationships between conditions, such pregnancy, obesity, or neurological diseases, with also highlighted.

Language: Английский

Citations

14
Type 2 diabetes and its genetic susceptibility are associated with increased severity and mortality of COVID-19 in UK Biobank DOI Creative Commons

A. Lee,

Ji-Eun Seo,

Seunghwan Park

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: Jan. 24, 2024

Abstract Type 2 diabetes (T2D) is known as one of the important risk factors for severity and mortality COVID-19. Here, we evaluate impact T2D its genetic susceptibility on COVID-19, using 459,119 individuals in UK Biobank. Utilizing polygenic scores (PRS) T2D, identified a significant association between or PRS, COVID-19 severity. We further discovered efficacy vaccination pivotal role T2D-related genetics pathogenesis severe Moreover, found that with those high PRS group had significantly increased rate. also observed rate SARS-CoV-2-infected patients was approximately to 7 times higher than not infected, depending time infection. These findings emphasize potential estimating

Language: Английский

Citations

6
Dysprosium tungstate incorporated on exfoliated layered molybdenum disulfide-based a flexible and wearable piezoelectric nanogenerator for the dual purpose of self-powered energy harvesting and a smart mask for human breath monitoring DOI
Ragu Sasikumar, Byungki Kim, Roshan Mangal Bhattarai

et al.

Nano Energy, Journal Year: 2023, Volume and Issue: 118, P. 109024 - 109024

Published: Oct. 24, 2023

Language: Английский

Citations

13
From Cell to Symptoms: The Role of SARS-CoV-2 Cytopathic Effects in the Pathogenesis of COVID-19 and Long COVID DOI Open Access
Pablo Gonzalez-Garcia, Ornella Fiorillo‐Moreno,

Eloina Zarate Peñate

et al.

Published: March 30, 2023

Severe Acute Respiratory Syndrome CoronaVirus 2 (SARS-CoV-2) infection triggers various events from molecular to tissue level, which in turn is given by the intrinsic characteristics of each patient. Given diversity characteristic cellular phenotype, possible cytopathic, and clinical effects are difficult predict, determines heterogeneity COVID-19 symptoms. The purpose this article provide a comprehensive review cytopathic SARS-CoV-2 on cell types, focusing development COVID-19, may lead, some patients, persistence symptoms after recovery disease, condition known as long COVID. We describe mechanisms underlying virus-host interactions, including alterations protein expression, intracellular signaling pathways, immune responses. In particular, highlights potential impact these cytopathies function outcomes, such dysregulation, neuropsychiatric disorders, organ damage. concludes discussing future directions for research implications management treatment Long-COVID.

Language: Английский

Citations

11
Better safe than sorry—Whole-genome sequencing indicates that missense variants are significant in susceptibility to COVID-19 DOI Creative Commons
Dawid Słomian, Joanna Szyda, Paula Dobosz

et al.

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(1), P. e0279356 - e0279356

Published: Jan. 20, 2023

Undoubtedly, genetic factors play an important role in susceptibility and resistance to COVID-19. In this study, we conducted the GWAS analysis. Out of 15,489,173 SNPs, identified 18,191 significant SNPs for severe 11,799 resistant phenotype, showing that a great number loci were different COVID-19 representations. The majority variants synonymous (60.56% severe, 58.46% phenotype) or located introns (55.77% 59.83% phenotype). We most outcome (in AJAP1 intron) COVID FIG4 intron). found no missense with potential causal function on COVID-19; however, two determined as phenotype PM20D1 LRP4 exons). None aforementioned study have been previously associated

Language: Английский

Citations

11
Identification of rare single nucleotide polymorphisms in the genome of hospitalized COVID-19 patients DOI Creative Commons
A. A. Perederiy, Andrey B. Komissarov, Daria Danilenko

et al.

Journal Infectology, Journal Year: 2025, Volume and Issue: 16(4), P. 68 - 77

Published: Jan. 16, 2025

The aim of this controlled study was to identify polymorphisms in the genome COVID­19 patients associated with frequency hospitalization. Materials and methods : Two groups were formed: main group – 56 COVID­19, hospitalized at least twice during period, control 107 for whom only one hospitalization confirmed period. Wholeexome sequencing residual nasopharyngeal swabs from performed on MGI platform, followed by bioinformatics analysis gene enrichment analysis. Result: For first time, exome oropharyngeal 163 using platform. In group, unique variants genetic identified, including six previously undescribed ones. Conclusion No statistically significantly single or multiple hospitalizations study. Nasopharyngeal can be used whole sequencing. Further studies are needed responsible susceptibility infectious diseases. whole­exome

Language: Английский

Citations

0
Identifying Baicalein as a Key Bioactive Compound in XueBiJing Targeting KEAP1: Implications for Antioxidant Effects DOI Creative Commons
Ting-Xuan Lin,

Xiaoxuan Cai,

Yibing Wang

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(3), P. 248 - 248

Published: Feb. 20, 2025

XueBiJing injection (XBJ) is renowned for its multi-target pharmacological effects, including immunomodulatory, antithrombotic, and antioxidant activities, offering potential therapeutic benefits patients with severe infections such as sepsis Coronavirus disease 2019 (COVID-19). Despite clinical effectiveness, the molecular targets mechanisms of XBJ remain unclear, warranting further investigation. This study aimed to identify key bioactive compounds in elucidate their mechanisms. The zebrafish model was first used evaluate anti-inflammatory effects XBJ, differentially expressed genes (DEGs) were identified by RNA sequencing network analysis. Network pharmacology analyze relationship between targets, docking kinetic simulation explore target binding ability compounds. Cellular Thermal Shift Assay-Western Blot (CETSA-WB) Surface Plasmon Resonance (SPR) verified interaction targets; finally, pathways confirmed gene silencing experiments. results reveal that significantly reduced neutrophil macrophage counts a dose-dependent manner, emphasizing potent effects. A transcriptomic analysis highlighted differential expression KEAP1/NRF2 pathway, HMOX1, SLC7A11, NQO1, TXNRD1. pinpointed KEAP1 central target, tanshinone IIA, baicalein, luteolin active modulating this pathway. Among these, IIA baicalein exhibited strong interactions KEAP1, which through simulations. Further validation showed directly demonstrated CETSA-WB SPR Additionally, experiments NRF2 reinforced crucial roles activating These findings collectively establish critical compound driving via pathway activation direct providing new insights into mechanism action XBJ.

Language: Английский

Citations

0
Assessment of genotoxic and cytotoxic effects in COVID-19 patients DOI
Işıl Deniz Alıravcı, Yusuf Haydar Ertekin, Nihan AKINCI KENANOĞLU

et al.

Çukurova medical journal (Online)/Çukurova medical journal, Journal Year: 2025, Volume and Issue: 50(1), P. 11 - 21

Published: March 25, 2025

Purpose: The objective of our study was to ascertain whether the SARS-CoV-2 virus exerts direct cytotoxic and genotoxic effects on human blood defence cells. Materials Methods: An in vitro analysis conducted assess using three established tests: mitotic index (MI), micronucleus (MN), comet assay (CA). These tests were applied samples from 101 patients. simultaneously analyzed polymerase chain reaction (PCR) test. population included patients all ages genders who presented outpatient clinic with symptoms suggestive a respiratory tract infection fever. Results: frequency MN lymphocytes COVID-19-infected (1.06) higher compared COVID-19-negative (0.68). Similarly, COVID-19-positive individuals, parameters such as tail length (3.67), moment (1.786), intensity showed significant increase negative control, indicating DNA damage. In cytotoxicity assessment, MI individuals (0.041) significantly lower than that controls (0.051). Gender did not influence cyto/genotoxicity (except for length) SARS-CoV-2-infected Among age groups, increased only middle-aged (26–36 years). Conclusion: has potential induce infected individuals.

Language: Английский

Citations

0