The “Ins and Outs and What-Abouts” of H2A.Z: A Tribute to C. David Allis

Journal of Biological Chemistry, Journal Year: 2025, Volume and Issue: 301(2), P. 108154 - 108154

Published: Jan. 4, 2025

In 2023, the brilliant chromatin biologist C. David Allis passed away leaving a large void in scientific community and broken hearts his family friends. With this review, we want to tribute Dave's enduring inspiration by focusing on histone variant H2A.Z, nucleosome component he was first discover as hv1 Tetrahymena. We summarize latest findings from past 5 years regarding mammalian H2A.Z histone, its deposition eviction mechanisms, roles transcriptional regulation, DNA damage repair, structure organization, embryonic development, well how deregulation or mutation(s) of chaperones contribute disease development. As Dave liked say 'Every amino acid matters'; discovery characterization functionally different H2A.Z's isoforms, which vary only three acids, prove him-once again-right.

Language: Английский

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Histone deacetylase in inflammatory bowel disease: novel insights

Therapeutic Advances in Gastroenterology, Journal Year: 2025, Volume and Issue: 18

Published: Jan. 1, 2025

Inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease, is characterized by chronic nonspecific intestinal inflammation. Despite considerable efforts, IBD remains a heavy burden on society human health, with increasing morbidity. Posttranslational modification, especially histone acetylation, key process in controlling DNA transcriptional activity. Histone deacetylases (HDACs) play vital role the mechanism of pathogenesis through nonhistone protein deacetylation. Herein, we present summary different categories HDACs as well HDAC inhibitors (HDACis) analyze inhibition alleviating along its mechanism, clinical potential HDACis treatment.

Language: Английский

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Epigenetics and Chromatin Remodeling

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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Modulation of Gene Expression by Substrate Stiffness via Ubiquitination of Histone H2B by Ubiquitin-Conjugating Enzyme E2A/B

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Language: Английский

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Quantitative Spatial Analysis of Chromatin Biomolecular Condensates using Cryo-Electron Tomography

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 2, 2024

Phase separation is an important mechanism to generate certain biomolecular condensates and organize the cell interior. Condensate formation function remain incompletely understood due difficulties in visualizing condensate interior at high resolution. Here we analyzed structure of biochemically reconstituted chromatin through cryo-electron tomography. We found that traditional blotting methods sample preparation were inadequate, high-pressure freezing plus focused ion beam milling was essential maintain integrity. To identify densely packed molecules within condensate, integrated deep learning-based segmentation with novel context-aware template matching. Our approaches developed on condensates, also effective condensed regions situ native chromatin. Using these methods, determined average nucleosomes 6.1 12 Å resolution systems, respectively, have a nearly random orientation distribution both cases. should be applicable diverse some cells.

Language: Английский

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Regulation of Catalase Expression and Activity by DhHog1 in the Halotolerant Yeast Debaryomyces hansenii Under Saline and Oxidative Conditions

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(11), P. 740 - 740

Published: Oct. 26, 2024

Efficient transcriptional regulation of the stress response is critical for microorganism survival. In yeast, stress-related gene expression, particularly antioxidant enzymes like catalases, mitigates reactive oxygen species such as hydrogen peroxide (H

Language: Английский

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