Identification of the key tryptophan metabolic characteristics of Lactiplantibacillus plantarum for aryl hydrocarbon receptor activation and ulcerative colitis alleviation

Food Research International, Journal Year: 2025, Volume and Issue: 203, P. 115766 - 115766

Published: Jan. 23, 2025

Language: Английский

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Investigating the expression of anti/pro-inflammatory cytokines in the pathogenesis and treatment of ulcerative colitis and its association with serum level of vitamin D

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 4, 2025

Ulcerative colitis is an idiopathic gastrointestinal disease described by chronic inflammation of the digestive system. Cytokines may be responsible for immunopathogenesis, mucosal and tissue damage, even treatment response. In addition to its role in calcium phosphorus homeostasis bone health, vitamin D immunomodulatory anti-inflammatory agent. Understanding cytokines lead improving pathogenesis this disease, therefore we aimed investigate relative gene expression pro- biopsy samples taken from affected area colon ulcerative patients association with serum levels. A total 47 were enrolled case-control study. The case group consisted 23 treatment-resistant colitis, control 24 responding routine treatment. Serum levels measured ELISA method. Real-time PCR was employed quantify groups. pro-inflammatory included tumor necrosis factor-α (TNF-α), interferon-gamma (IFN-γ), interleukin-1β (IL-1β), IL-6, IL-8, IL-17 A, IL-33, while IL-10, IL-35, TGF-β. Data are showed as mean ± standard deviation (SD), p values < 0.05 considered statistically significant. age 45.88 18.51 years, that 41.30 13.01 years. TNF-α, IFN-γ, IL-1β, TGF-β, groups did not exhibit significant differences (p > 0.05). However, principal cytokines, including elevated compared who responded treatments. No correlation observed between present study identify a or Therefore, treatments had no effect on these patients. Additionally, related Despite need further research protective pathological roles D, regular screening early complementary beneficial reducing inflammatory symptoms

Language: Английский

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Low-grade Chronic Inflammation: A Shared Mechanism for Chronic Diseases

Physiology, Journal Year: 2024, Volume and Issue: 40(1), P. 000 - 000

Published: July 30, 2024

Inflammation is an important physiological response of the organism to restore homeostasis upon pathogenic or damaging stimuli. However, persistence harmful trigger a deficient resolution process can evolve into state low-grade, chronic inflammation. This condition strongly associated with development several increasingly prevalent and serious conditions, such as obesity, cancer, cardiovascular diseases, elevating overall morbidity mortality worldwide. The current pandemic diseases underscores need address inflammation, its mechanisms, potential preventive measures limit widespread impact. present review discusses knowledge research gaps regarding association between low-grade inflammation focusing on digestive cancer. We examine art in selected aspects topic propose future directions approaches for field.

Language: Английский

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Uncovering the Beneficial Role of Limosilactobacillus fermentum E7 Exhibiting Antioxidant Activity in Ameliorating DSS-Induced Ulcerative Colitis in a Murine Model

Foods, Journal Year: 2025, Volume and Issue: 14(1), P. 137 - 137

Published: Jan. 6, 2025

Background: Ulcerative colitis (UC) is a chronic intestinal disease of growing global concern. Bacteria associated with fermented food or probiotics regulate immune and inflammatory responses, playing key role in homeostasis. Results: Five relatively good antioxidant effects, namely Lactiplantibacillus plantarum H6, Latilactobacillus sakei QC9, Limosilactobacillus fermentum E7, Bacillus subtills D1, licheniformis Q13, were screened out from 30 strains through vitro assays. The five had varying degrees alleviating effects on UC mice improved various physiological indicators, such as oxidative stress parameters histopathological sections. Q13 more pronounced. Furthermore, E7 effectively regulated mouse microbiota composition, increased short-chain fatty acid concentration, promoted the expression anti-inflammatory factors, interleukin 10 (IL-10), while suppressing that pro-inflammatory 1β (IL-1β), 6 (IL-6), tumor necrosis factor α (TNF-α). Meanwhile, D1 only exhibited partial effects. Finally, tight junction proteins colon tissues. Conclusions: showed superior efficacy to other UC, offering novel therapeutic prospects for safer effective management UC.

Language: Английский

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Intestinal tissue levels of anti-TNF alpha, antibodies, and cytokines in paediatric Crohn disease

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 7, 2025

The aim was to explore factors associated with intestinal tissue levels of anti-TNF alpha (anti-TNF), antibodies, and cytokines in pediatric patients Crohn Disease (CD). In a prospective exploratory study CD undergoing ileocecal resection or colonoscopy between 6/2020 1/2023, we analysed anti-TNF, (TNF-α, IL-17, IL-1β, IFN-γ) from biopsies. Mixed-effects regression models, adjusted for potential confounders, were used. Data 27 (18 females, 66.7%) analysed. Fourteen (52%) received adalimumab (ADA) thirteen infliximab (IFX), median therapy duration 17 (IQR 4.5–41.5) months. Higher free found macroscopically inflamed compared non-inflamed (β = 3.42, 95% CI 1.05–6.10). No significant association serum (β= −0.06, − 0.70–0.58). Patients treated longer had increased IL-17 0.19, 0.05–0.33), independent disease age. IFN-γ linked both follow-up length. Our shows significantly higher drug tissue. Long-term treatment has been levels, suggesting possible impact on the cytokine response pathway. We did not observe relationship levels.

Language: Английский

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Probiotic Bacillus pumilus LV149 enhances gut repair, modulates microbiota, and alters transcriptome in DSS-induced colitis mice

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 7, 2025

Gut microbiota dysbiosis significantly impacts ulcerative colitis (UC) progression and exacerbation. Probiotics show promise in UC management. This study evaluated the effects of different doses Bacillus pumilus LV149, an aquatic-derived probiotic, on gut injury repair male C57BL/6 mice with dextran sulfate sodium (DSS)-induced investigated underlying mechanisms. was induced by allowing free access to a 3% DSS solution for 7 days, concurrent daily oral gavage either low (LV149-L, 1 × 108 CFU/day/mouse) or high (LV149-H, 109 dose LV149. The were assessed through physiological parameters, intestinal barrier integrity, inflammation, composition, transcriptomic changes. LV149 improved pathological symptoms, including weight loss disease activity index (DAI), reduced colon shortening dose-dependent manner inflammatory damage. intervention also restored function upregulating mucins, goblet cell counts, tight junction proteins (ZO-1, occludin, claudin-1) colonic tissue, along reducing serum lipopolysaccharide (LPS) levels. Notably, only LV149-H decreased expression pro-inflammatory cytokines TNF-α, IL-1β, IL-6, while both increased anti-inflammatory cytokine IL-10 tissue. further modulated microbiota, increasing beneficial bacteria pathogenic populations. Transcriptomic analysis indicated that LV149-L may exert via IL-17 signaling pathway, whereas appears act JAK-STAT pathway. demonstrated particularly at higher dose, effectively mitigated DSS-induced modulating enhancing inflammation. underscored LV149-H's potential as therapeutic agent due its stronger properties gut-protective effects.

Language: Английский

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Biological characteristics, immune infiltration and drug prediction of PANoptosis related genes and possible regulatory mechanisms in inflammatory bowel disease

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 15, 2025

PANoptosis is one of several modes programmed cell death (PCD) and plays an important role in many inflammatory immune diseases. The bowel disease (IBD) currently unknown. Differentially expressed PANoptosis-related genes (DE-PRGs) were identified, pathway enrichment analyses performed. LASSO regression model construction, a nomogram model, calibration curves, ROC DCA curves used to evaluate the predictive value model. Predicts transcription factors (TFs) small-molecule drugs DE-PRGs analysed. Model immuno-infiltration features IBD include 12 genes: OGT, TLR2, GZMB, TLR4, PPIF, YBX3, CASP5, BCL2L1, CASP6, MEFV, GSDMB BAX. analysis suggested that these related TNF signalling, NF-κB, pyroptosis necroptosis. Machine learning identified three GZMB CASP5. have strong value. Immuno-infiltration revealed infiltration was increased patients with IBD, closely various cells. TFs associated RELA, NFKB1, HIF1A, TP53 SP1. In addition, Connectivity Map (CMap) database top 10 compounds, including buspirone, chloroquine, spectinomycin chlortetracycline. This study indicate good ability for IBD. Moreover, may mediate process through pyroptosis, necroptosis mechanisms. These results present new horizon research treatment

Language: Английский

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Tofacitinib and budesonide treatment affect stemness and chemokine release in IBD patient-derived colonoids

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 30, 2025

Restoration of the intestinal epithelial barrier is crucial for achieving mucosal healing, therapeutic goal inflammatory bowel disease (IBD). During homeostasis, renewal maintained by crypt stem cells and progenitors that cease to divide as they differentiate into mature colonocytes. Inflammation a major effector damage in IBD has been found affect stemness, regeneration cellular functions. However, impact immune cell-modulating drugs on homeostasis repair poorly understood. It likely these will have distinct mechanisms action (MOA) epithelium relevant vary among patients. We investigated effects pan-Janus Kinase (JAK) inhibitor tofacitinib corticosteroid budesonide uninflamed TNF + Poly(I:C) stimulated human colon organoids (colonoids) from healthy donors IBD-patients. Our findings reveal although both exhibit anti-inflammatory effects, increased colonoid size proliferation during differentiation, promoted stemness. In contrast, decreased showed no consistent effect or study demonstrates value employing colonoids investigate how inter-individual variations healing personalized treatment.

Language: Английский

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MAIT cell homing in intestinal homeostasis and inflammation

Science Advances, Journal Year: 2025, Volume and Issue: 11(6)

Published: Feb. 7, 2025

Mucosa-associated invariant T (MAIT) cells are a large population of unconventional widely distributed in the human gastrointestinal tract. Their homing to gut is central maintaining mucosal homeostasis and immunity. This review discusses potential mechanisms that guide MAIT intestinal mucosa during inflammation, emphasizing roles chemokines, chemokine receptors, tissue adhesion molecules. The influence microbiota on cell different regions also discussed. Last, we introduce how organoid technology offers potentially valuable approach advance our understanding by providing more physiologically relevant model mimics tissue. These models may enable detailed investigation gut-specific cells. By regulation gut, avenues for therapeutic interventions targeting inflammatory conditions such as bowel diseases (IBD) emerge.

Language: Английский

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Synergistic role of gut-microbial L-ornithine in enhancing ustekinumab efficacy for Crohn’s disease

Cell Metabolism, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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