Towards improved biofilm models

Nature Reviews Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 7, 2024

Language: Английский

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Metabolic interplay between Proteus mirabilis and Enterococcus faecalis facilitates polymicrobial biofilm formation and invasive disease

mBio, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 30, 2024

ABSTRACT Biofilms play an important role in the development and pathogenesis of catheter-associated urinary tract infection (CAUTI). Proteus mirabilis Enterococcus faecalis are common CAUTI pathogens that persistently co-colonize catheterized form biofilms with increased biomass antibiotic resistance. In this study, we uncover metabolic interplay drives biofilm enhancement examine contribution to severity. Through compositional proteomic analyses, determined increase stems from protein fraction polymicrobial biofilm. We further observed enrichment proteins associated ornithine arginine metabolism compared single-species biofilms. show arginine/ornithine antiport by E. promotes biosynthesis P. , ultimately driving content without affecting viability either species. disrupting alters profile prevents enhancement, defect was complemented supplementation exogenous ornithine. a murine model CAUTI, did not contribute colonization but required for incidence stone formation bacteremia occurs during . Thus, between co-colonizing species may represent viable strategy reducing risk bacteremia. IMPORTANCE Chronic infections often involve antibiotic-resistant communities include multiple different microbes, which pose challenge effective treatment. tract, potential long periods time interactions them can lead more severe disease outcomes. identified metabolite L-ornithine as key mediator disease-enhancing two challenging pathogens, Disrupting ornithine-mediated therefore prevent decrease disease.

Language: Английский

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Anomalous diffusion of nanoparticles in the spatially heterogeneous biofilm environment

iScience, Journal Year: 2023, Volume and Issue: 26(6), P. 106861 - 106861

Published: May 13, 2023

Biofilms contain extracellular polymeric substances (EPS) that provide structural support and restrict penetration of antimicrobial treatment. To overcome limited penetration, functionalized nanoparticles (NPs) have been suggested as carriers for delivery. Using microscopy, we evaluate the diffusion in function structure Salmonella biofilms. We observe anomalous heterogeneous mobility NPs resulting distinct distribution depended on biofilm structure. Through Brownian dynamics modeling with spatially varying viscosity around bacteria, demonstrated spatial gradients diffusivity generate viscous sinks trap near bacteria. This model replicates characteristic signature vertical biofilm. From a treatment perspective, our work indicates both level EPS can impact NP drug delivery, where low levels might benefit delivery by immobilizing closer to bacteria higher hamper due shielding effects.

Language: Английский

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Mechanics limits ecological diversity and promotes heterogeneity in confined bacterial communities

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(20)

Published: May 10, 2024

Multispecies bacterial populations often inhabit confined and densely packed environments where spatial competition determines the ecological diversity of community. However, role mechanical interactions in shaping ecology is still poorly understood. Here, we study a model system consisting two nonmotile Escherichia coli bacteria competing within open, monolayer microchannels. The competitive dynamics observed to be biphasic: After seeding, either one strain rapidly fixates or both strains orient into spatially stratified, stable communities. We find that with other cells local constraints influence resulting community unexpected ways, severely limiting overall communities while simultaneously allowing for establishment stable, heterogeneous displaying disparate growth rates. Surprisingly, have high probability coexisting even when has significant advantage. A more coccus morphology shown provide selective advantage, but agent-based simulations indicate this due hydrodynamic adhesion effects microchannel not from breaking nematic ordering. Our observations are qualitatively reproduced by simple Pólya urn model, which suggests generality our findings population highlights importance early colonization conditions on These results fundamental insights determinants dense ecosystems exclusion central as organized biofilms intestinal crypts.

Language: Английский

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Metabolic interplay between Proteus mirabilis and Enterococcus faecalis facilitates polymicrobial biofilm formation and invasive disease

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: March 18, 2023

Summary Polymicrobial biofilms play an important role in the development and pathogenesis of CAUTI. Proteus mirabilis Enterococcus faecalis are common CAUTI pathogens that persistently co-colonize catheterized urinary tract form with increased biomass antibiotic resistance. In this study, we uncover metabolic interplay drives biofilm enhancement examine contribution to severity. Through compositional proteomic analyses, determined increase stems from protein fraction polymicrobial matrix. We further observed enrichment proteins associated ornithine arginine metabolism compared single-species biofilms. show L-ornithine secretion by E. promotes biosynthesis P. mirabilis, disruption abrogates see vitro leads significant decreases infection severity dissemination a murine model.

Language: Английский

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