Perspective: The viscoelastic properties of biofilm infections and mechanical interactions with phagocytic immune cells DOI Creative Commons
Marilyn J. Wells, Rebecca Schneider, Bikash Bhattarai

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2023, Volume and Issue: 13

Published: Feb. 16, 2023

Biofilms are viscoelastic materials that a prominent public health problem and cause of most chronic bacterial infections, in large part due to their resistance clearance by the immune system. Viscoelastic combine both solid-like fluid-like mechanics, properties biofilms an emergent property intercellular cohesion characterizing biofilm state (planktonic bacteria do not have equivalent property). However, how mechanical related recalcitrant disease they cause, specifically phagocytic system, remains almost entirely unstudied. We believe this is important gap ripe for range investigations. Here we present overview what known about infections interactions with mechanics potential relationship phagocytosis, give illustrative example one biofilm-pathogen (Pseudomonas aeruginosa) which most-studied context. hope inspire investment growth relatively-untapped field research, has reveal as targets therapeutics meant enhance efficacy

Language: Английский

A Photomodulable Bacteriophage‐Spike Nanozyme Enables Dually Enhanced Biofilm Penetration and Bacterial Capture for Photothermal‐Boosted Catalytic Therapy of MRSA Infections DOI Creative Commons
Haibin Wu, Min Wei, Shen Hu

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(24)

Published: June 13, 2023

Nanozymes, featuring intrinsic biocatalytic effects and broad-spectrum antimicrobial properties, are emerging as a novel antibiotic class. However, prevailing bactericidal nanozymes face challenging dilemma between biofilm penetration bacterial capture capacity, significantly impeding their antibacterial efficacy. Here, this work introduces photomodulable nanozyme (ICG@hMnO

Language: Английский

Citations

52
Bacterial genome size and gene functional diversity negatively correlate with taxonomic diversity along a pH gradient DOI Creative Commons
Cong Wang, Qingyi Yu, Niu‐Niu Ji

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Nov. 17, 2023

Abstract Bacterial gene repertoires reflect adaptive strategies, contribute to ecosystem functioning and are limited by genome size. However, functional diversity does not necessarily correlate with taxonomic because average size may vary community. Here, we analyse (by shotgun metagenomics) 16S rRNA amplicon sequencing) investigate soil bacterial communities along a natural pH gradient in 12 tropical, subtropical, temperate forests. We find that decrease, whereas increases, as rises from acid neutral; result, negatively correlated. The repertoire of acid-adapted oligotrophs is enriched functions signal transduction, cell motility, secretion system, degradation complex compounds, while neutral pH-adapted copiotrophs energy metabolism membrane transport. Our results indicate mismatch between can arise when environmental factors (such pH) select for strategies affect distributions.

Language: Английский

Citations

52
Drinking water sources as hotspots of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs): Occurrence, spread, and mitigation strategies DOI

Shengnan Li,

Brim Stevy Ondon,

Shih‐Hsin Ho

et al.

Journal of Water Process Engineering, Journal Year: 2023, Volume and Issue: 53, P. 103907 - 103907

Published: June 16, 2023

Language: Английский

Citations

51
Root colonization by beneficial rhizobacteria DOI Creative Commons
Yunpeng Liu, Zhihui Xu, Lin Chen

et al.

FEMS Microbiology Reviews, Journal Year: 2023, Volume and Issue: 48(1)

Published: Dec. 12, 2023

Rhizosphere microbes play critical roles for plant's growth and health. Among them, the beneficial rhizobacteria have potential to be developed as biofertilizer or bioinoculants sustaining agricultural development. The efficient rhizosphere colonization of these is a prerequisite exerting their plant functions, but colonizing process underlying mechanisms not been thoroughly reviewed, especially nonsymbiotic rhizobacteria. This review systematically analyzed root compared it with that symbiotic pathogenic bacteria. also highlighted approaches improve efficiency proposed study rhizobacterial from holistic perspective microbiome under more natural conditions.

Language: Английский

Citations

49
How biofilm changes our understanding of cleaning and disinfection DOI Creative Commons
Jean‐Yves Maillard, Isabella Centeleghe

Antimicrobial Resistance and Infection Control, Journal Year: 2023, Volume and Issue: 12(1)

Published: Sept. 7, 2023

Biofilms are ubiquitous in healthcare settings. By nature, biofilms less susceptible to antimicrobials and associated with healthcare-associated infections (HAI). Resistance of biofilm is multifactorial the presence a matrix composed extracellular polymeric substances eDNA, being major contributing factor. The usual multispecies composition environmental can also impact on antimicrobial efficacy. In settings, two main types present: hydrated biofilms, for example, drains parts some medical devices equipment, dry (DSB) surfaces possibly devices. act as reservoir pathogens including multi-drug resistant organisms their elimination requires different approaches. control (drain) should be informed by reduction or microbial bioburden together measuring regrowth time. DSB measured combination decrease bacterial transfer post-intervention. Failure increases risk HAI, but not solely responsible disinfection failure shortcoming. limited number standardised efficacy tests hindrance end users manufacturers, whilst Europe there no approved standard protocols. Education stakeholders about ad hoc tests, often academic thus paramount, achieve better

Language: Английский

Citations

46
Microenvironment‐Responsive Hydrogels with Detachable Skin Adhesion and Mild‐Temperature Photothermal Property for Chronic Wound Healing DOI
Huimin Geng, Xiaoyue Zheng, Yulin Zhang

et al.

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(51)

Published: Aug. 27, 2023

Abstract Chronic wounds have emerged as a global healthcare burden that inability to heal within an expected time frame owing the complex pathophysiological microenvironment, bacterial infection, multidrug resistance, and fragile skin tissue. Tailored features of chronic wounds, multifunctional hydrogel (GA) composed ethylenediamine‐modified gelatin (Gela‐amino) oxidized sodium alginate (Alg─CHO) is reported. The thermosensitivity Schiff base bonds endow GA hydrogels with physiological temperature‐enhanced nonirritating tissue adhesion low‐temperature‐triggered nondestructive separation, beneficial for management vulnerable sensitive wounds. In addition, exhibit pH‐responsive degradation adapt acidic wound microenvironment. Under near‐infrared (NIR) irradiation, DNase I enzyme indocyanine green‐loaded (DI@GA) efficiently eradicate drug‐resistant bacteria biofilm subsequently exert antibacterial effect attributed bioactity mild‐temperature photothermal therapy (≈45 °C). Remarkably, DI@GA/NIR accelerate diabetic recovery regeneration by dispersing biofilm, killing bacteria, inhibiting inflammation, promoting collagen deposition, improving angiogenesis. advanced without addition antibiotics are promising act skin‐friendly dressings rescuing infectious stalled

Language: Английский

Citations

45
Combating Bacterial Biofilms: Current and Emerging Antibiofilm Strategies for Treating Persistent Infections DOI Creative Commons
Ahmed G. Abdelhamid, Ahmed E. Yousef

Antibiotics, Journal Year: 2023, Volume and Issue: 12(6), P. 1005 - 1005

Published: June 3, 2023

Biofilms are intricate multicellular structures created by microorganisms on living (biotic) or nonliving (abiotic) surfaces. Medically, biofilms often lead to persistent infections, increased antibiotic resistance, and recurrence of infections. In this review, we highlighted the clinical problem associated with biofilm infections focused current emerging antibiofilm strategies. These strategies directed at disrupting quorum sensing, which is crucial for formation, preventing bacterial adhesion surfaces, impeding aggregation in viscous mucus layers, degrading extracellular polymeric matrix, developing nanoparticle-based antimicrobial drug complexes target cells within core. It important acknowledge, however, that use agents faces obstacles, such as limited effectiveness vivo, potential cytotoxicity host cells, propensity elicit resistance targeted biofilm-forming microbes. Emerging next generation strategies, rely multipronged approaches, were highlighted, these benefit from advances nanotechnology, synthetic biology, discovery. The assessment mitigation presented here, could guide future initiatives toward innovative therapeutic Enhancing efficacy specificity some via careful investigations, under conditions closely mimic characteristics human body, bridge gap between laboratory research practical application.

Language: Английский

Citations

43
Beneficial applications of biofilms DOI
Laura-Alina Philipp, Katja Bühler, Roland Ulber

et al.

Nature Reviews Microbiology, Journal Year: 2023, Volume and Issue: 22(5), P. 276 - 290

Published: Nov. 13, 2023

Language: Английский

Citations

43
Staphylococcus aureus biofilm: Formulation, regulatory, and emerging natural products-derived therapeutics DOI Creative Commons
Xiying Wu, Huan Wang, Juan Xiong

et al.

Biofilm, Journal Year: 2024, Volume and Issue: 7, P. 100175 - 100175

Published: Jan. 1, 2024

Staphylococcus aureus can readily form biofilm which enhances the drug-resistance, resulting in life-threatening infections involving different organs. Biofilm formation occurs due to a series of developmental events including bacterial adhesion, aggregation, maturation, and dispersion, are controlled by multiple regulatory systems. Rapidly increasing research development outcomes on natural products targeting S. and/or regulation led an emergent application active phytochemicals combinations. This review aimed at providing in-depth understanding mechanisms for aureus, outlining most important antibiofilm strategies potential targets products, summarizing latest progress combating with plant-derived products. These findings provided further evidence novel drugs clinical therapies.

Language: Английский

Citations

37
Mechanisms of antimicrobial resistance in biofilms DOI Creative Commons
H. Liu,

Emma L. Prentice,

Mark Webber

et al.

npj Antimicrobials and Resistance, Journal Year: 2024, Volume and Issue: 2(1)

Published: Oct. 1, 2024

Most bacteria in nature exist aggregated communities known as biofilms, and cells within a biofilm demonstrate major physiological changes compared to their planktonic counterparts. Biofilms are associated with many different types of infections which can have severe impacts on patients. Infections involving component often chronic highly recalcitrant antibiotic therapy result intrinsic physical factors including extracellular matrix production, low growth rates, altered target production efficient exchange resistance genes. This review describes the lifecycle, phenotypic characteristics biofilm, contribution persister biofilms tolerance antimicrobials. We also describe how evolve transfer genes biofilms. Multispecies various interactions, cooperation competition, between species antimicrobials polymicrobial discussed.

Language: Английский

Citations

36