PLoS Genetics,
Journal Year:
2025,
Volume and Issue:
21(2), P. e1011560 - e1011560
Published: Feb. 7, 2025
Plasmid
conjugation
is
a
major
route
for
the
dissemination
of
antibiotic
resistances
and
adaptive
genes
among
bacterial
populations.
Obtaining
precise
rates
thus
key
to
understanding
how
spread.
typically
modeled
as
density-dependent
process,
where
formation
new
transconjugants
depends
on
rate
encounters
between
donor
receptor
cells.
By
analyzing
dynamics
at
different
cell
concentrations,
here
we
show
that
this
assumption
only
holds
very
low
densities.
At
higher
becomes
limited
by
engagement
time,
interval
required
two
successful
matings.
therefore
follows
Holling´s
Type
II
functional
response,
characterized
encounter
which
represent,
respectively,
density
frequency-dependent
limits
plasmid
transmission.
Our
results
demonstrate
these
parameters
are
characteristic
transfer
machinery,
rather
than
entire
genome,
they
robust
environmental
transcriptional
perturbation.
Precise
parameterization
will
contribute
better
propagation
antimicrobial
resistances.
Journal of Medicine Surgery and Public Health,
Journal Year:
2024,
Volume and Issue:
2, P. 100081 - 100081
Published: March 2, 2024
Antimicrobial
resistance
(AMR)
is
a
critical
global
health
issue
driven
by
antibiotic
misuse
and
overuse
in
various
sectors,
leading
to
the
emergence
of
resistant
microorganisms.
The
history
AMR
dates
back
discovery
penicillin,
with
rise
multidrug-resistant
pathogens
posing
significant
challenges
healthcare
systems
worldwide.
antibiotics
human
animal
health,
as
well
agriculture,
contributes
spread
genes,
creating
"Silent
Pandemic"
that
could
surpass
other
causes
mortality
2050.
affects
both
humans
animals,
treating
infections.
Various
mechanisms,
such
enzymatic
modification
biofilm
formation,
enable
microbes
withstand
effects
antibiotics.
lack
effective
threatens
routine
medical
procedures
lead
millions
deaths
annually
if
left
unchecked.
economic
impact
substantial,
projected
losses
trillions
dollars
financial
burdens
on
agriculture.
Artificial
intelligence
being
explored
tool
combat
improving
diagnostics
treatment
strategies,
although
data
quality
algorithmic
biases
exist.
To
address
effectively,
One
Health
approach
considers
human,
animal,
environmental
factors
crucial.
This
includes
enhancing
surveillance
systems,
promoting
stewardship
programs,
investing
research
development
for
new
antimicrobial
options.
Public
awareness,
education,
international
collaboration
are
essential
combating
preserving
efficacy
future
generations.
Critical Reviews in Microbiology,
Journal Year:
2024,
Volume and Issue:
50(5), P. 896 - 921
Published: Feb. 21, 2024
Healthy
state
is
priority
in
today's
world
which
can
be
achieved
using
effective
medicines.
But
due
to
overuse
and
misuse
of
antibiotics,
a
menace
resistance
has
increased
pathogenic
microbes.
World
Health
Organization
(WHO)
announced
ESKAPE
pathogens
(
Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: May 29, 2024
Abstract
Bacterial
pathogens
carrying
multidrug
resistance
(MDR)
plasmids
are
a
major
threat
to
human
health.
The
acquisition
of
antibiotic
genes
(ARGs)
in
is
often
facilitated
by
mobile
genetic
elements
that
copy
or
translocate
ARGs
between
DNA
molecules.
agglomeration
generates
islands
comprising
multiple
ARGs.
However,
whether
the
emergence
restricted
specific
MDR
plasmid
lineages
remains
understudied.
Here
we
show
biased
towards
large
lineages.
Analyzing
6784
2441
Escherichia
,
Salmonella
and
Klebsiella
isolates,
quantify
84%
found
islands.
We
furthermore
observe
rapid
evolution
ARG
combinations
Most
regions
identified
as
shared
among
closely
related
but
rarely
distantly
plasmids.
Our
results
suggest
presence
barriers
for
dissemination
lineages,
which
properties,
host
range
evolutionary
history.
attributed
workings
operate
within
framework
existing
Journal of Nanobiotechnology,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: April 15, 2024
Abstract
Hydrogels
are
a
class
of
highly
absorbent
and
easily
modified
polymer
materials
suitable
for
use
as
slow-release
carriers
drugs.
Gene
therapy
is
specific
can
overcome
the
limitations
traditional
tissue
engineering
techniques
has
significant
advantages
in
repair.
However,
therapeutic
genes
often
affected
by
cellular
barriers
enzyme
sensitivity,
carrier
loading
essential.
Therapeutic
gene
hydrogels
well
these
difficulties.
Moreover,
gene-therapeutic
have
made
considerable
progress.
This
review
summarizes
recent
research
on
treatment
damage
through
summary
most
current
frontiers.
We
initially
introduce
classification
their
cross-linking
methods,
followed
detailed
overview
types
modifications
genes,
discussion
characterization
features,
design
release,
an
applications
engineering.
Finally,
we
provide
comments
look
forward
to
shortcomings
future
directions
therapy.
hope
that
this
article
will
researchers
related
fields
with
more
comprehensive
systematic
strategies
repair
further
promote
development
field
Graphical
abstract
Eco-Environment & Health,
Journal Year:
2025,
Volume and Issue:
unknown, P. 100136 - 100136
Published: Jan. 1, 2025
Microplastics
(MPs)
and
antibiotic
resistance
genes
(ARGs)
pose
significant
challenges
to
the
One
Health
framework
due
their
intricate
multifaceted
ecological
environmental
impacts.
However,
understanding
of
how
MP
properties
influence
ARG
prevalence
in
mariculture
sediments
remains
limited.
Herein,
polystyrene
(PS)
polyvinyl
chloride
(PVC)
MPs
with
different
sizes
(20-120
μm
0.5-2.0
mm)
were
selected
evaluate
impacts
underlying
mechanisms
driving
ARGs
dissemination.
The
results
showed
that
PS
PVC
increased
relative
abundance
by
1.41-2.50-fold
2.01-2.84-fold,
respectively,
compared
control,
particularly
high-risk
genes.
polymer
type
effect
was
identified
as
more
influential
than
size
sediment
resistome
evolution.
shifted
microbial
community
assembly
from
stochastic
deterministic
processes,
thus
enriching
host
pathogens.
Furthermore,
highly
hydrophobic
not
only
recruited
bacteria
colonization
but
also
facilitated
exchange
within
plastisphere.
exogenous
additives
released
(e.g.,
heavy
metals,
bisphenol
A,
tridecyl
ester)
particles
synergistically
promoted
conjugative
transfer
inducing
oxidative
stress
enhancing
cell
membrane
permeability.
These
findings
revealed
characteristics
spread
marine
benthic
ecosystems,
underscoring
importance
mitigating
pollution
maintain
ecosystem
health,
prevent
zoonotic
diseases,
balance
global
health.
Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: Jan. 27, 2024
Abstract
Natural
microbial
ecosystems
harbor
substantial
diversity
of
competing
species.
Explaining
such
is
challenging,
because
in
classic
theories
it
extremely
infeasible
for
a
large
community
species
to
stably
coexist
homogeneous
environments.
One
important
aspect
mostly
overlooked
these
theories,
however,
that
microbes
commonly
share
genetic
materials
with
their
neighbors
through
horizontal
gene
transfer
(HGT),
which
enables
the
dynamic
change
growth
rates
due
fitness
effects
mobile
elements
(MGEs).
Here,
we
establish
framework
competition
by
accounting
flow
among
microbes.
Combining
theoretical
derivation
and
numerical
simulations,
show
many
conditions
HGT
can
surprisingly
overcome
biodiversity
limit
predicted
model
allow
coexistence
competitors,
enabling
neutrality
In
contrast
static
proposed
previous
maintained
highly
stable
against
random
perturbations
fitness.
Our
work
highlights
importance
considering
when
addressing
fundamental
ecological
questions
world
has
broad
implications
design
engineering
complex
consortia.
Environment International,
Journal Year:
2024,
Volume and Issue:
191, P. 108972 - 108972
Published: Aug. 22, 2024
Overutilization
and
misuse
of
antibiotics
in
recent
decades
markedly
intensified
the
rapid
proliferation
diffusion
antibiotic
resistance
genes
(ARGs)
within
environment,
thereby
elevating
ARGs
to
status
a
global
public
health
crisis.
Recognizing
that
soil
acts
as
critical
reservoir
for
ARGs,
environmental
researchers
have
made
great
progress
exploring
sources,
distribution,
spread
soil.
However,
microscopic
state
micro-interfacial
behavior
remains
inadequately
understood.
In
this
study,
we
reviewed
behaviors
antibiotic-resistant
bacteria
(ARB)
porous
media,
predominantly
including
migration-deposition,
adsorption,
biofilm
formation.
Meanwhile,
proliferation,
degradation
were
identified
primary
soil,
with
component
serving
significant
determinant.
Our
work
contributes
further
comprehension
microstates
processes
ARB
environments
offers
theoretical
foundation
managing
mitigating
risks
associated
ARG
contamination.