Encounter rates and engagement times limit the transmission of conjugative plasmids DOI Creative Commons
Jorge Rodríguez-Grande, Yelina Ortiz, Daniel Garcia-Lopez

et al.

PLoS Genetics, Journal Year: 2025, Volume and Issue: 21(2), P. e1011560 - e1011560

Published: Feb. 7, 2025

Plasmid conjugation is a major route for the dissemination of antibiotic resistances and adaptive genes among bacterial populations. Obtaining precise rates thus key to understanding how spread. typically modeled as density-dependent process, where formation new transconjugants depends on rate encounters between donor receptor cells. By analyzing dynamics at different cell concentrations, here we show that this assumption only holds very low densities. At higher becomes limited by engagement time, interval required two successful matings. therefore follows Holling´s Type II functional response, characterized encounter which represent, respectively, density frequency-dependent limits plasmid transmission. Our results demonstrate these parameters are characteristic transfer machinery, rather than entire genome, they robust environmental transcriptional perturbation. Precise parameterization will contribute better propagation antimicrobial resistances.

Language: Английский

Antimicrobial resistance: Impacts, challenges, and future prospects DOI Creative Commons
Sirwan Khalid Ahmed, Safin Hussein, Karzan Qurbani

et al.

Journal of Medicine Surgery and Public Health, Journal Year: 2024, Volume and Issue: 2, P. 100081 - 100081

Published: March 2, 2024

Antimicrobial resistance (AMR) is a critical global health issue driven by antibiotic misuse and overuse in various sectors, leading to the emergence of resistant microorganisms. The history AMR dates back discovery penicillin, with rise multidrug-resistant pathogens posing significant challenges healthcare systems worldwide. antibiotics human animal health, as well agriculture, contributes spread genes, creating "Silent Pandemic" that could surpass other causes mortality 2050. affects both humans animals, treating infections. Various mechanisms, such enzymatic modification biofilm formation, enable microbes withstand effects antibiotics. lack effective threatens routine medical procedures lead millions deaths annually if left unchecked. economic impact substantial, projected losses trillions dollars financial burdens on agriculture. Artificial intelligence being explored tool combat improving diagnostics treatment strategies, although data quality algorithmic biases exist. To address effectively, One Health approach considers human, animal, environmental factors crucial. This includes enhancing surveillance systems, promoting stewardship programs, investing research development for new antimicrobial options. Public awareness, education, international collaboration are essential combating preserving efficacy future generations.

Language: Английский

Citations

258
Antibiotic resistance: a global crisis, problems and solutions DOI

Rupesh Aggarwal,

Pooja Mahajan,

Sameeksha Pandiya

et al.

Critical Reviews in Microbiology, Journal Year: 2024, Volume and Issue: 50(5), P. 896 - 921

Published: Feb. 21, 2024

Healthy state is priority in today's world which can be achieved using effective medicines. But due to overuse and misuse of antibiotics, a menace resistance has increased pathogenic microbes. World Health Organization (WHO) announced ESKAPE pathogens (

Language: Английский

Citations

38
Antibiotic resistance: A key microbial survival mechanism that threatens public health DOI

Amna Abbas,

Alexandra Barkhouse,

Dirk Hackenberger

et al.

Cell Host & Microbe, Journal Year: 2024, Volume and Issue: 32(6), P. 837 - 851

Published: June 1, 2024

Language: Английский

Citations

38
The evolution of antibiotic resistance islands occurs within the framework of plasmid lineages DOI Creative Commons
Yiqing Wang, Tal Dagan

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 29, 2024

Abstract Bacterial pathogens carrying multidrug resistance (MDR) plasmids are a major threat to human health. The acquisition of antibiotic genes (ARGs) in is often facilitated by mobile genetic elements that copy or translocate ARGs between DNA molecules. agglomeration generates islands comprising multiple ARGs. However, whether the emergence restricted specific MDR plasmid lineages remains understudied. Here we show biased towards large lineages. Analyzing 6784 2441 Escherichia , Salmonella and Klebsiella isolates, quantify 84% found islands. We furthermore observe rapid evolution ARG combinations Most regions identified as shared among closely related but rarely distantly plasmids. Our results suggest presence barriers for dissemination lineages, which properties, host range evolutionary history. attributed workings operate within framework existing

Language: Английский

Citations

22
Developing hydrogels for gene therapy and tissue engineering DOI Creative Commons
Chunyu Su, Dini Lin, Xinyu Huang

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: April 15, 2024

Abstract Hydrogels are a class of highly absorbent and easily modified polymer materials suitable for use as slow-release carriers drugs. Gene therapy is specific can overcome the limitations traditional tissue engineering techniques has significant advantages in repair. However, therapeutic genes often affected by cellular barriers enzyme sensitivity, carrier loading essential. Therapeutic gene hydrogels well these difficulties. Moreover, gene-therapeutic have made considerable progress. This review summarizes recent research on treatment damage through summary most current frontiers. We initially introduce classification their cross-linking methods, followed detailed overview types modifications genes, discussion characterization features, design release, an applications engineering. Finally, we provide comments look forward to shortcomings future directions therapy. hope that this article will researchers related fields with more comprehensive systematic strategies repair further promote development field Graphical abstract

Language: Английский

Citations

20
Microplastics enhance the prevalence of antibiotic resistance genes in mariculture sediments by enriching host bacteria and promoting horizontal gene transfer DOI Creative Commons
Yifan Liu, Liuqingqing Liu, Xiao Wang

et al.

Eco-Environment & Health, Journal Year: 2025, Volume and Issue: unknown, P. 100136 - 100136

Published: Jan. 1, 2025

Microplastics (MPs) and antibiotic resistance genes (ARGs) pose significant challenges to the One Health framework due their intricate multifaceted ecological environmental impacts. However, understanding of how MP properties influence ARG prevalence in mariculture sediments remains limited. Herein, polystyrene (PS) polyvinyl chloride (PVC) MPs with different sizes (20-120 μm 0.5-2.0 mm) were selected evaluate impacts underlying mechanisms driving ARGs dissemination. The results showed that PS PVC increased relative abundance by 1.41-2.50-fold 2.01-2.84-fold, respectively, compared control, particularly high-risk genes. polymer type effect was identified as more influential than size sediment resistome evolution. shifted microbial community assembly from stochastic deterministic processes, thus enriching host pathogens. Furthermore, highly hydrophobic not only recruited bacteria colonization but also facilitated exchange within plastisphere. exogenous additives released (e.g., heavy metals, bisphenol A, tridecyl ester) particles synergistically promoted conjugative transfer inducing oxidative stress enhancing cell membrane permeability. These findings revealed characteristics spread marine benthic ecosystems, underscoring importance mitigating pollution maintain ecosystem health, prevent zoonotic diseases, balance global health.

Language: Английский

Citations

6
Innovative ceramic solutions: Boosting antibacterial power of DD30.85Mg0.15O for environmental applications DOI

Dikra Bouras,

Mamoun Fellah, R. Barillé

et al.

Inorganic Chemistry Communications, Journal Year: 2025, Volume and Issue: unknown, P. 114046 - 114046

Published: Feb. 1, 2025

Language: Английский

Citations

5
Horizontal gene transfer is predicted to overcome the diversity limit of competing microbial species DOI Creative Commons
Shiben Zhu, Juken Hong, Teng Wang

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 27, 2024

Abstract Natural microbial ecosystems harbor substantial diversity of competing species. Explaining such is challenging, because in classic theories it extremely infeasible for a large community species to stably coexist homogeneous environments. One important aspect mostly overlooked these theories, however, that microbes commonly share genetic materials with their neighbors through horizontal gene transfer (HGT), which enables the dynamic change growth rates due fitness effects mobile elements (MGEs). Here, we establish framework competition by accounting flow among microbes. Combining theoretical derivation and numerical simulations, show many conditions HGT can surprisingly overcome biodiversity limit predicted model allow coexistence competitors, enabling neutrality In contrast static proposed previous maintained highly stable against random perturbations fitness. Our work highlights importance considering when addressing fundamental ecological questions world has broad implications design engineering complex consortia.

Language: Английский

Citations

14
Prophage-encoded antibiotic resistance genes are enriched in human-impacted environments DOI Creative Commons
Hanpeng Liao, Chen Liu, Shungui Zhou

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 27, 2024

Language: Английский

Citations

14
Micro-interfacial behavior of antibiotic-resistant bacteria and antibiotic resistance genes in the soil environment: A review DOI Creative Commons

Jibao Deng,

W. Zhang, Lingyu Zhang

et al.

Environment International, Journal Year: 2024, Volume and Issue: 191, P. 108972 - 108972

Published: Aug. 22, 2024

Overutilization and misuse of antibiotics in recent decades markedly intensified the rapid proliferation diffusion antibiotic resistance genes (ARGs) within environment, thereby elevating ARGs to status a global public health crisis. Recognizing that soil acts as critical reservoir for ARGs, environmental researchers have made great progress exploring sources, distribution, spread soil. However, microscopic state micro-interfacial behavior remains inadequately understood. In this study, we reviewed behaviors antibiotic-resistant bacteria (ARB) porous media, predominantly including migration-deposition, adsorption, biofilm formation. Meanwhile, proliferation, degradation were identified primary soil, with component serving significant determinant. Our work contributes further comprehension microstates processes ARB environments offers theoretical foundation managing mitigating risks associated ARG contamination.

Language: Английский

Citations

11