Sensitivity and specificity of immunohistochemistry for the diagnosis of filamentous fungal infections DOI

Victoria L Thomas,

Alvaro C. Laga, Isaac H. Solomon

et al.

American Journal of Clinical Pathology, Journal Year: 2025, Volume and Issue: unknown

Published: May 10, 2025

Abstract Objectives Many fungal species share overlapping morphologic features in tissue sections, preventing reliable identification and optimal treatment. We sought to determine whether immunohistochemistry (IHC) using a panel of commercially available antibodies could effectively distinguish between fungi commonly encountered anatomic pathology specimens. Methods Anti-Aspergillus, anti-Rhizopus, anti-Candida IHC was performed on formalin-fixed, paraffin-embedded sections from 24 cases with infections identified by culture or sequencing (including 4 polyfungal infections). Results Anti-Aspergillus positive 6 Aspergillus focally 1 Candida negative all Fusarium, Scedosporium, Rhizopus, Mucor species, yielding overall sensitivity 100% specificity 95%. Anti-Rhizopus Rhizopus 3 other cases, 71% 100%. Anti-Candida showed some cross-reactivity resulting 0% specificity. Conclusions highly sensitive specific its ability similar-appearing hyaline molds, including Fusarium Scedosporium species. moderately specific, while but had minimal

Language: Английский

Focus on fungi DOI
Iliyan D. Iliev, Gordon D. Brown, Petra Bächer

et al.

Cell, Journal Year: 2024, Volume and Issue: 187(19), P. 5121 - 5127

Published: Sept. 1, 2024

Language: Английский

Citations

18
Advances in Coordination Chemistry of Schiff Base Complexes: A Journey from Nanoarchitectonic Design to Biomedical Applications DOI
Ahmad Abd‐El‐Aziz,

Zexuan Li,

Xinyue Zhang

et al.

Topics in Current Chemistry, Journal Year: 2025, Volume and Issue: 383(1)

Published: Feb. 3, 2025

Language: Английский

Citations

2
Deciphering the role of mitochondria in human fungal drug resistance DOI Creative Commons
Yuanyuan Ma, Yachun Zhou,

Tianyuan Jia

et al.

Mycology&#58 An International Journal on Fungal Biology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 14

Published: March 10, 2025

Language: Английский

Citations

1
Computational analysis of Ayurvedic metabolites for potential treatment of drug-resistant Candida auris DOI Creative Commons
Mohibullah Shah,

Mahnoor Zia,

Iqra Ahmad

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: March 13, 2025

This study explored the effectiveness of secondary metabolites referred traditional Ayurvedic plants in treating fungal infections, particularly targeting Candida auris . Recognized as a global health threat, this fungus is notorious for its resistance to several antifungal treatments. The inhibition lanosterol 14α-demethylase causes depletion ergosterol, ultimately resulting cell growth. A total 469 metabolites, including alkaloids, flavonoids, and tannins from plants, were screened against CYP51 (PDB ID: 4UYL) using molecular docking. Key active site residues, namely HIS461, CYS463, TYR122, targeted inhibit ergosterol synthesis, with VNI employed benchmark findings. Shortlisted underwent physicochemical analysis, ADMET analyses, principles medicinal chemistry, which confirmed through pharmacokinetic simulations. Further, investigated dynamics (MD) co-crystalized VNI, trans-p-coumaric acid, MCPHB [(r)-n-(1’-methoxycarbonyl-2’-phenylethyl)-4-hydroxybenzamide] evaluate RMSD, RMSF, Rg, SASA, cross-correlation residue motions, PCA, free energy decomposition. top compounds demonstrated favorable drug-like criteria. They exhibited good absorption potential high gastrointestinal uptake. Distribution metabolism manageable low risks drug-drug interactions. Excretion profiles indicated proper clearance, toxicity assessments showed cardiovascular issues. results stable interactions acid MCPHB, suggesting that all ligands maintain binding protein, preserves structural integrity across systems. comprehensive approach suggests these natural medicine could potentially serve primary agents diseases, pending further validation controlled vitro vivo clinical trials.

Language: Английский

Citations

1
Uncovering the Diversity and Distribution of Fungal Infections in Rwanda: Assessing Risks and Documenting Knowledge and Policy Gaps DOI Open Access
Claude Mambo Muvunyi, Jean Claude Semuto Ngabonziza,

Florence Masaisa

et al.

Published: July 10, 2024

Fungal infections (FIs) are spreading globally causing a significant public health concern. However, its documentation remains sparse in Africa, particularly Rwanda. This literature review, aims to assess the risk and document current gaps evidence policy. It summarizes published data about FIs Rwanda, including ten reports between 1972-and-2022. Blastomyces, Candida, Cryptococcus, Histoplasma, Microsporum, Pneumocystis, Rhinosporidium, Trichophyton caused human infections. These primarily affected brain, respiratory, urinary reproductive organs, they were diagnosed using culturing, histopathology, immunology, and/or microscopic techniques. Our findings provide an overview of diversity distribution FIs, highlighting limitations country’s diagnostic capacity surveillance system for FIs. Also, indicating severe inform policymaking, guide strategic planning intervention, underscoring urgent need build national fungal diagnosis, surveillance, research. Raising awareness among public, scientific community, healthcare providers, policymakers crucial. Furthermore, this report reveals threats on food insecurity A Multisectoral One Health Strategy is essential research intervention determine reduce safety impacts pathogens human, animal, environment.

Language: Английский

Citations

5
Diagnosing fungal infections in clinical practice: a narrative review DOI
Sarah Sedik,

Stella Wolfgruber,

Martin Hoenigl

et al.

Expert Review of Anti-infective Therapy, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 13, 2024

Invasive fungal infections (IFI) present a major medical challenge, with an estimated 6.5 million cases annually, resulting in 3.8 deaths. Pathogens such as Aspergillus spp. Candida Mucorales Cryptococcus and other fungi species contribute to these infections, posing risks immunocompromised individuals. Early accurate diagnosis is crucial for effective treatment better patient outcomes.

Language: Английский

Citations

5
Distinct evolutionary trajectories following loss of RNA interference in Cryptococcus neoformans DOI Creative Commons
Jun Huang, Connor J. Larmore, Shelby Priest

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(47)

Published: Nov. 13, 2024

While increased mutation rates typically have negative consequences in multicellular organisms, hypermutation can be advantageous for microbes adapting to the environment. Previously, we identified two hypermutator

Language: Английский

Citations

5
PHI-base – the multi-species pathogen–host interaction database in 2025 DOI Creative Commons
Martin Urban, Alayne Cuzick, James Seager

et al.

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: 53(D1), P. D826 - D838

Published: Nov. 26, 2024

The Pathogen-Host Interactions Database (PHI-base) has, since 2005, provided manually curated genes from fungal, bacterial and protist pathogens that have been experimentally verified to important pathogenicity, virulence and/or effector functions during different types of interactions involving human, animal, plant, invertebrate fungal hosts. PHI-base provides phenotypic annotation genotypic information for both native model host interactions, including gene alterations do not alter the phenotype interaction. In this article, we describe major updates PHI-base. latest version PHI-base, 4.17, contains a 19% increase in 23% relative 4.12 (released September 2022). We also unification data 4 with new curation workflow (PHI-Canto), which forms first complete release 5.0. Additionally, adding support Frictionless Data framework datasets, ways sharing interaction Ensembl database, an analysis conserved orthologous increasing variety research studies make use 4.17 is freely available at www.phi-base.org 5.0 phi5.phi-base.org.

Language: Английский

Citations

5
AIDS-related mycoses: advances, challenges, and future directions DOI
Rachael Dangarembizi, J. Claire Hoving, David R. Boulware

et al.

Trends in Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Extracellular Vesicles as Biomarkers in Infectious Diseases DOI Creative Commons

C. Cruz,

H Sodawalla, Thalachallour Mohanakumar

et al.

Biology, Journal Year: 2025, Volume and Issue: 14(2), P. 182 - 182

Published: Feb. 11, 2025

Extracellular vesicles (EVs) are nanosized that secreted by all cells into the extracellular space. EVs involved in cell-to-cell communication and can be found different bodily fluids (bronchoalveolar lavage fluid, sputum, urine), tissues, circulation; composition of reflects physiological condition releasing cell. The ability to use from for minimally invasive detection monitor diseases makes them an attractive target. carry a snapshot cell's internal state, they serve as powerful biomarkers diagnosing diseases. also play role body's immune pathogen responses. Pathogens, such bacteria viruses, exploit enhance their survival spread evade system. Changes number or contents signal presence infection, offering potential avenue developing new diagnostic methods infectious Ongoing research this area aims address current challenges range diseases, including infections There is limited literature on development using existing molecular biology approaches. We aim gap reviewing recent EV-related investigations disease studies.

Language: Английский

Citations

0