Extracellular vesicles set the stage for brain plasticity and recovery by multimodal signalling DOI Creative Commons
Dirk M. Hermann, Luca Peruzzotti‐Jametti, Bernd Giebel

et al.

Brain, Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 28, 2023

Abstract Extracellular vesicles (EVs) are extremely versatile naturally occurring membrane particles that convey complex signals between cells. EVs of different cellular sources capable inducing striking therapeutic responses in neurological disease models. Differently from pharmacological compounds act by modulating defined signalling pathways, EV-based therapeutics possess multiple abilities via a variety effectors, thus allowing the modulation processes may have very potent effects on brain tissue recovery. When applied vivo experimental models diseases, revealed remarkable immune responses, cell metabolism and neuronal plasticity. This multimodal neuroimmune networks profoundly influences highly synergistic context-dependent way. Ultimately, EV-mediated restoration functions helps to set stage for With this review we first outline current understanding mechanisms action EVs, describing how released various identify their targets recipient Then, applicable key conditions such as stroke, sclerosis neurodegenerative diseases presented. Pathways deserve attention specific contexts discussed. We subsequently showcase considerations about EV biodistribution delineate genetic engineering strategies aiming at enhancing uptake signalling. By sketching broad view EV-orchestrated plasticity recovery, finally define possible future clinical applications propose necessary information be provided ahead trials. Our goal is provide steppingstone can used critically discuss next generation diseases.

Language: Английский

Therapeutic potential in rheumatic diseases of extracellular vesicles derived from mesenchymal stromal cells DOI
Giuliana Minani Bertolino,

Marie Maumus,

Christian Jørgensen

et al.

Nature Reviews Rheumatology, Journal Year: 2023, Volume and Issue: 19(11), P. 682 - 694

Published: Sept. 4, 2023

Language: Английский

Citations

30
tRNA therapeutics for genetic diseases DOI
Jeff Coller, Zoya Ignatova

Nature Reviews Drug Discovery, Journal Year: 2023, Volume and Issue: 23(2), P. 108 - 125

Published: Dec. 4, 2023

Language: Английский

Citations

29
Extracellular vesicle-circEHD2 promotes the progression of renal cell carcinoma by activating cancer-associated fibroblasts DOI Creative Commons
Tao He, Qiansheng Zhang, Peng Xu

et al.

Molecular Cancer, Journal Year: 2023, Volume and Issue: 22(1)

Published: July 22, 2023

Abstract Background The encapsulation of circular RNAs (circRNAs) into extracellular vesicles (EVs) enables their involvement in intercellular communication and exerts an influence on the malignant advancement various tumors. However, regulatory role EVs-circRNA renal cell carcinoma (RCC) remains elusive. Methods vitro vivo functional experiments were implemented to measure effects circEHD2 phenotype RCC. EVs-circEHD2 activation fibroblasts was assessed by collagen contraction assay, western blotting, enzyme-linked immunosorbent assay (ELISA). mechanism investigated RNA pull-down immunoprecipitation, chromatin isolation purification, luciferase co-immunoprecipitation assay. Results We demonstrated that upregulated RCC tissues serum EVs patients with metastasis. Silencing inhibited tumor growth vivo. Mechanistic studies indicated FUS -binding protein (FUS) accelerated cyclization circEHD2, then interacts tyrosine 3-monooxygenase/tryptophan 5-monooxygenase eta (YWHAH), which acts as a bridge recruit Yes1-associated transcriptional regulator (YAP) promoter SRY-box transcription factor 9 (SOX9); this results sustained SOX9. Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2B1) regulates package EVs, transmits fibroblasts, converting cancer-associated (CAFs). Activated CAFs promote metastasis secreting pro-inflammatory cytokines such IL-6. Furthermore, antisense oligonucleotides (ASOs) targeting exhibited strong inhibition Conclusions circEHD2/YWHAH/YAP/SOX9 signaling pathway accelerates facilitates CAFs. Our suggest may be useful biomarker therapeutic target for

Language: Английский

Citations

27
CRISPR-Cas9 delivery strategies with engineered extracellular vesicles DOI Creative Commons
Yaoyao Lu, Kelly Godbout, Gabriel Lamothe

et al.

Molecular Therapy — Nucleic Acids, Journal Year: 2023, Volume and Issue: 34, P. 102040 - 102040

Published: Sept. 26, 2023

Therapeutic genome editing has the potential to cure diseases by directly correcting genetic mutations in tissues and cells. Recent progress CRISPR-Cas9 systems led breakthroughs gene tools because of its high orthogonality, versatility, efficiency. However, safe effective administration target organs patients is a major hurdle. Extracellular vesicles (EVs) are endogenous membranous particles secreted spontaneously all They key actors cell-to-cell communication, allowing exchange select molecules such as proteins, lipids, RNAs induce functional changes recipient Recently, EVs have displayed their for trafficking system during or after formation. In this review, we highlight recent developments EV loading, surface functionalization, strategies increasing efficiency delivering tissues, organs, cells eventual use therapies.

Language: Английский

Citations

27
Extracellular vesicles set the stage for brain plasticity and recovery by multimodal signalling DOI Creative Commons
Dirk M. Hermann, Luca Peruzzotti‐Jametti, Bernd Giebel

et al.

Brain, Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 28, 2023

Abstract Extracellular vesicles (EVs) are extremely versatile naturally occurring membrane particles that convey complex signals between cells. EVs of different cellular sources capable inducing striking therapeutic responses in neurological disease models. Differently from pharmacological compounds act by modulating defined signalling pathways, EV-based therapeutics possess multiple abilities via a variety effectors, thus allowing the modulation processes may have very potent effects on brain tissue recovery. When applied vivo experimental models diseases, revealed remarkable immune responses, cell metabolism and neuronal plasticity. This multimodal neuroimmune networks profoundly influences highly synergistic context-dependent way. Ultimately, EV-mediated restoration functions helps to set stage for With this review we first outline current understanding mechanisms action EVs, describing how released various identify their targets recipient Then, applicable key conditions such as stroke, sclerosis neurodegenerative diseases presented. Pathways deserve attention specific contexts discussed. We subsequently showcase considerations about EV biodistribution delineate genetic engineering strategies aiming at enhancing uptake signalling. By sketching broad view EV-orchestrated plasticity recovery, finally define possible future clinical applications propose necessary information be provided ahead trials. Our goal is provide steppingstone can used critically discuss next generation diseases.

Language: Английский

Citations

26