Nano Letters,
Journal Year:
2023,
Volume and Issue:
23(4), P. 1591 - 1599
Published: Feb. 1, 2023
Cells
release
extracellular
vesicles
(EVs)
as
the
carriers
for
intercellular
communications
to
regulate
life
activities.
Particularly,
it
is
increasingly
apparent
that
mechanical
forces
play
an
essential
role
in
biological
systems.
The
nanomechanical
properties
of
EVs
and
their
dynamics
cancer
development
are
still
not
fully
understood.
Herein,
with
use
atomic
force
microscopy
(AFM),
signatures
from
liquid
biopsies
hematologic
patients
were
unraveled.
Single
native
probed
by
AFM
under
aqueous
conditions.
elastic
viscous
measured
visualized
correlate
EV
mechanics
geometry.
Experimental
results
remarkably
reveal
significant
differences
among
multiple
myeloma
patients,
lymphoma
healthy
volunteers.
study
unveils
unique
cancers,
which
will
benefit
studies
diagnosis
prognosis
translational
significance.
Journal of Orthopaedic Translation,
Journal Year:
2023,
Volume and Issue:
39, P. 124 - 134
Published: March 1, 2023
The
cerebrospinal
fluid
(CSF),
which
surrounds
the
brain
and
spinal
cord,
is
predominantly
produced
by
choroid
plexus
of
ventricle.
Although
CSF-derived
extracellular
vesicles
(CSF-EVs)
may
be
utilized
as
diagnostic
prognostic
indicators
for
illnesses
central
nervous
system
(CNS),
it
uncertain
if
CSF-EVs
have
an
impact
on
neurological
function
after
cord
injury
(SCI).
Here,
we
isolated
EVs
using
ultracentrifugation
extracting
CSF
from
Bama
miniature
pigs.
We
then
combined
with
hydrogel
put
cord's
surface.
To
determine
had
mice's
neurofunctional
recovery,
behavioral
evaluations
were
employed.
Both
in
vitro
vivo,
effect
angiogenesis
was
assessed.
investigated
whether
stimulated
PI3K/AKT
pathway
to
alter
PI3K
inhibitor
LY294002.
successfully
identified
transmission
electron
microscope
(TEM),
nano-tracking
analysis
(NTA),
western
blot.
could
ingested
vascular
endothelial
cells
proved
vivo
imaging
immunofluorescence.
demonstrated
that
derived
pigs
SCI
(SCI-EVs)
showed
a
better
promoting
regeneration
compared
receiving
laminectomy
(Sham-EVs).
Behavioral
assessments
SCI-EVs
dramatically
enhance
motor
sensory
mice
SCI.
Western
blot
suggested
promote
activating
signaling
pathway,
pro-angiogenetic
attenuated
application
LY294002
(PI3K
inhibitor).
Our
study
revealed
hence
improving
recovery
SCI,
offer
potential
novel
therapeutic
options
acute
This
promotion
exosomes,
provide
approach
treatment
injury.
Nano Letters,
Journal Year:
2023,
Volume and Issue:
23(4), P. 1591 - 1599
Published: Feb. 1, 2023
Cells
release
extracellular
vesicles
(EVs)
as
the
carriers
for
intercellular
communications
to
regulate
life
activities.
Particularly,
it
is
increasingly
apparent
that
mechanical
forces
play
an
essential
role
in
biological
systems.
The
nanomechanical
properties
of
EVs
and
their
dynamics
cancer
development
are
still
not
fully
understood.
Herein,
with
use
atomic
force
microscopy
(AFM),
signatures
from
liquid
biopsies
hematologic
patients
were
unraveled.
Single
native
probed
by
AFM
under
aqueous
conditions.
elastic
viscous
measured
visualized
correlate
EV
mechanics
geometry.
Experimental
results
remarkably
reveal
significant
differences
among
multiple
myeloma
patients,
lymphoma
healthy
volunteers.
study
unveils
unique
cancers,
which
will
benefit
studies
diagnosis
prognosis
translational
significance.
