Biomarkers of aging

Science China Life Sciences, Journal Year: 2023, Volume and Issue: 66(5), P. 893 - 1066

Published: April 11, 2023

Language: Английский

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Inter-organ crosstalk during development and progression of type 2 diabetes mellitus

Nature Reviews Endocrinology, Journal Year: 2023, Volume and Issue: 20(1), P. 27 - 49

Published: Oct. 16, 2023

Language: Английский

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Simultaneous subset tracing and miRNA profiling of tumor-derived exosomes via dual-surface-protein orthogonal barcoding

Science Advances, Journal Year: 2023, Volume and Issue: 9(40)

Published: Oct. 4, 2023

The clinical potential of miRNA-based liquid biopsy has been largely limited by the heterogeneous sources in plasma and tedious assay processes. Here, we develop a precise robust one-pot called dual-surface-protein-guided orthogonal recognition tumor-derived exosomes situ profiling microRNAs (SORTER) to detect exosomal miRNAs enhance diagnostic accuracy prostate cancer (PCa). SORTER uses two allosteric aptamers against marker CD63 tumor EpCAM create an labeling barcode achieve selective sorting tumor-specific exosome subtypes. Furthermore, labeled on initiated targeted membrane fusion with liposome probes import miRNA detection reagents, enabling sensitive miRNAs. With signature six miRNAs, differentiated PCa benign prostatic hyperplasia 100%. Notably, reached 90.6% classification metastatic nonmetastatic PCa. We envision that will promote adaptability biopsy.

Language: Английский

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Cellular senescence of renal tubular epithelial cells in renal fibrosis

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 28, 2023

Renal fibrosis (RF) is the common pathological manifestation of virtually all chronic kidney diseases (CKD) and one major causes end-stage renal disease (ESRD), but pathogenesis which still unclear. tubulointerstitial lesions have been identified as a key hallmark RF pathology. tubular epithelial cells are resident tubulointerstitium play an important role in recovery versus following injury. Studies recent years shown that senescence can accelerate progression fibrosis. Oxidative stress(OS), telomere attrition DNA damage cell senescence. Current interventions therapeutic strategies for cellular include calorie restriction routine exercise, Klotho, senolytics, senostatics, other related drugs. This paper provides overview mechanisms signaling pathways including Wnt/β-catenin/RAS, Nrf2/ARE STAT-3/NF-κB pathway involved therapies targeting future potential patients. These findings may offer promise further treatment CKD.

Language: Английский

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An Extracellular miRNA‐Responsive Artificial Receptor via Dynamic DNA Nano‐assembly for Biomarker‐Driven Therapy

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(31)

Published: June 20, 2023

MicroRNAs (miRNAs) have emerged as promising diagnostic biomarkers and therapeutic targets in various diseases. However, there is currently a lack of molecular strategies that can effectively use disease-associated extracellular miRNAs input signals to drive functions. Herein, we present modular programmable miRNA-responsive chimeric DNA receptor (miRNA-CDR) capable biomarker-driven therapy. By grafting nanodevice on natural membrane via aptamer anchoring, miRNA-CDR sense miRNA levels autonomously induce dimerization-mediated activation the complementary-mediated strand displacement reaction-induced dynamic assembly. The sequence programmability allows it respond user-defined with tunable sensitivity. Moreover, versatile customizable reprogram desirable signaling output adapting designated receptor, such MET FGFR1. Using mouse model drug-induced acute liver injury (DILI), demonstrate functionality designer rewiring recognition DILI-elevated miR-122 promote hepatocytes for situ repair function restoration. Our synthetic platform provides novel device enabling cellular response, potentially paving way improving precision cell therapy regenerative medicine.

Language: Английский

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Kidney fibrosis: Emerging diagnostic and therapeutic strategies

Molecular Aspects of Medicine, Journal Year: 2023, Volume and Issue: 93, P. 101206 - 101206

Published: Aug. 3, 2023

Language: Английский

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miR-147 Represses NDUFA4, Inducing Mitochondrial Dysfunction and Tubular Damage in Cold Storage Kidney Transplantation

Journal of the American Society of Nephrology, Journal Year: 2023, Volume and Issue: 34(8), P. 1381 - 1397

Published: May 22, 2023

Cold storage-associated transplantation (CST) injury occurs in renal transplant from deceased donors, the main organ source. The pathogenesis of CST remains poorly understood, and effective therapies are not available. This study has demonstrated an important role microRNAs revealed changes microRNA expression profiles. Specifically, microRNA-147 (miR-147) is consistently elevated during mice dysfunctional grafts humans. Mechanistically, NDUFA4 (a key component mitochondrial respiration complex) identified as a direct target miR-147. By repressing NDUFA4, miR-147 induces damage tubular cell death. Blockade overexpression reduce improve graft function, unveiling new therapeutic targets kidney transplantation.

Language: Английский

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METTL3-Mediated N6-Methyladenosine mRNA Modification and cGAS-STING Pathway Activity in Kidney Fibrosis

Journal of the American Society of Nephrology, Journal Year: 2024, Volume and Issue: 35(10), P. 1312 - 1329

Published: June 10, 2024

Chemical modifications on RNA profoundly affect function and regulation. m6A, the most abundant modification in eukaryotes, plays a pivotal role diverse cellular processes disease mechanisms. However, its importance is understudied human CKD samples regarding influence pathological

Language: Английский

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Toward Precision Medicine: Exploring the Landscape of Biomarkers in Acute Kidney Injury

Biomolecules, Journal Year: 2024, Volume and Issue: 14(1), P. 82 - 82

Published: Jan. 8, 2024

Acute kidney injury (AKI) remains a complex challenge with diverse underlying pathological mechanisms and etiologies. Current detection methods predominantly rely on serum creatinine, which exhibits substantial limitations in specificity poses the issue of late-stage injury. In this review, we propose an up-to-date comprehensive summary advancements that identified novel biomarker candidates blood urine ideal criteria for AKI biomarkers such as renal specificity, mechanistic insight, prognostic capacity, affordability. Recently not only indicate location but also offer valuable insights into range processes, encompassing reduced glomerular filtration rate, tubular function, inflammation, adaptive response to The clinical applications are becoming extensive serving relevant tools distinguishing acute necrosis from other conditions. Also, these can significant risk progression chronic disease CKD context transplantation. Integration practice has potential improve early diagnosis revolutionize design trials, offering endpoints therapeutic interventions enhancing patient care outcomes.

Language: Английский

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Novel SERS Signal Amplification Strategy for Ultrasensitive and Specific Detection of Spinal Cord Injury-Related miRNA

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(2), P. 736 - 744

Published: Feb. 12, 2024

The expression of microRNA (miRNA) changes in many diseases plays an important role the diagnosis, treatment, and prognosis diseases. Spinal cord injury (SCI) is a serious disease central nervous system, accompanied by inflammation, cell apoptosis, neuronal necrosis, axonal rupture, demyelination, other pathological processes, resulting impaired sensory motor functions patients. Studies have shown that miRNA has changed after SCI, miRNAs participate pathophysiological process treatment SCI. Therefore, quantitative analysis monitoring were great significance for diagnosis Through SCI-related chord plot, we screened out miRNA-21-5p miRNA-let-7a with higher correlation. However, traditional detection strategies, it still challenge to achieve fast, accurate, sensitive complex biological environments. most frequently used method detecting polymerase chain reaction (PCR), but disadvantages such as being time-consuming cumbersome. In this paper, novel SERS sensor serum cerebrospinal fluid (CSF) was developed. probe eventually formed sandwich-like structure Fe3O4@hpDNA@miRNA@hpDNA@GNCs target miRNAs, which had high specificity stability. This achieved wide range from 1 fM nM good linear relationship. limits (LOD) 0.015 0.011 fM, respectively. new strategy realized long-term vivo. It expected become powerful biomolecule tool will provide ideas

Language: Английский

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