Satellite glial cells promote regenerative growth in sensory neurons

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: Sept. 29, 2020

Peripheral sensory neurons regenerate their axon after nerve injury to enable functional recovery. Intrinsic mechanisms operating in are known regulate repair, but whether satellite glial cells (SGC), which completely envelop the neuronal soma, contribute regeneration remains unexplored. Using a single cell RNAseq approach, we reveal that SGC distinct from Schwann and share similarities with astrocytes. Nerve elicits changes expression of genes related fatty acid synthesis peroxisome proliferator-activated receptor (PPARα) signaling. Conditional deletion synthase (Fasn) impairs regeneration. The PPARα agonist fenofibrate rescues impaired mice lacking Fasn SGC. These results indicate activity downstream FASN contributes promote adult peripheral nerves highlight neuron its surrounding coat form unit orchestrates repair.

Language: Английский

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Central nervous system regeneration

Cell, Journal Year: 2022, Volume and Issue: 185(1), P. 77 - 94

Published: Jan. 1, 2022

Language: Английский

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A Brief Overview of Global Trends in MSC-Based Cell Therapy

Stem Cell Reviews and Reports, Journal Year: 2022, Volume and Issue: 18(5), P. 1525 - 1545

Published: March 28, 2022

Language: Английский

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Plasma membrane integrity: implications for health and disease

BMC Biology, Journal Year: 2021, Volume and Issue: 19(1)

Published: April 13, 2021

Abstract Plasma membrane integrity is essential for cellular homeostasis. In vivo, cells experience plasma damage from a multitude of stressors in the extra- and intra-cellular environment. To avoid lethal consequences, are equipped with repair pathways to restore integrity. Here, we assess whole-body perspective. We highlight role tissue-specific health disease examine across diverse cell types. Furthermore, outline impact genetic environmental factors on how these contribute pathogenesis different tissues.

Language: Английский

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Functional nanomaterials in peripheral nerve regeneration: Scaffold design, chemical principles and microenvironmental remodeling

Materials Today, Journal Year: 2021, Volume and Issue: 51, P. 165 - 187

Published: Oct. 12, 2021

Neuronal microenvironment imbalance is associated with successive and irreversible pathophysiological changes insufficient functional restoration after peripheral nerve injury. Conventional neural-supporting scaffolds result in unsatisfactory curative effects due to lack of biomimetic nanotechnology designs biochemical or physicochemical modifications. Consequently, they fail rational facile remodeling the imbalanced growth microenvironment, cannot recover neural structure function. In recent years, increasing knowledge neuronal injury-associated a number novel strategies are applied enhancing natures nanomaterial-based for tissue engineering. These nanoscale can trigger factor secretion aggregation through surface modification, regulate ATP synthesis hydrolysis, switch between oxidation reduction states, activate ion channels stimulate electrical signals under certain biophysical cues. determine cell fate by modulating their viability, development cycles during regeneration process. this review, we systematically summarize studies on scaffold design nanomaterials, basic topological, physical properties, nanotechnology-based balanced nutritional regarding four key factors, including immune response, intraneural vascularization, bioenergetic metabolism bioelectrical conduction order provide ideas inspiration nanomedicine-based therapy.

Language: Английский

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Neuroinflammation, Microglia and Implications for Retinal Ganglion Cell Survival and Axon Regeneration in Traumatic Optic Neuropathy

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: March 4, 2022

Traumatic optic neuropathy (TON) refers to a pathological condition caused by direct or indirect insult the nerves, which often leads partial permanent vision deficit due massive loss of retinal ganglion cells (RGCs) and their axonal fibers. Retinal microglia are immune-competent residing in retina. In rodent models nerve crush (ONC) injury, resident gradually become activated, form end-to-end alignments vicinity degenerating RGC axons, actively internalized them. Some activated adopt an amoeboid morphology that engulf dying RGCs after ONC. injured nerve, contribute myelin debris clearance at lesion site. However, phagocytic capacity is extremely poor therefore cellular largely ineffective. The presence growth-inhibitory glial scar formed reactive astrocytes inhibit regeneration accounts for visual function recovery patients with TON. this Review, we summarize current understanding survival axon Resident play key role facilitating Wallerian degeneration subsequent they also responsible producing pro-inflammatory cytokines, chemokines, oxygen species possess neurotoxic effects on RGCs. Intraocular inflammation triggers influx blood-borne myeloid produce oncomodulin promote regeneration. intraocular induces chronic neuroinflammation exacerbates secondary tissue damages limits Activated required proliferation oligodendrocyte precursor (OPCs); however, sustained activation suppress differentiation OPCs into mature oligodendrocytes remyelination injury. Collectively, controlled infiltrating facilitate repair. Recent advance single-cell RNA-sequencing identification microglia-specific markers could improve our microglial biology development novel therapeutic strategies aiming switch microglia’s phenotype foster neuroprotection.

Language: Английский

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Photoacoustic Carbon Nanotubes Embedded Silk Scaffolds for Neural Stimulation and Regeneration

ACS Nano, Journal Year: 2022, Volume and Issue: 16(2), P. 2292 - 2305

Published: Jan. 31, 2022

Neural interfaces using biocompatible scaffolds provide crucial properties, such as cell adhesion, structural support, and mass transport, for the functional repair of nerve injuries neurodegenerative diseases. stimulation has also been found to be effective in promoting neural regeneration. This work provides a generalized strategy integrate photoacoustic (PA) into hydrogel nanocomposite approach. Specifically, polyethylene glycol (PEG)-functionalized carbon nanotubes (CNT), highly efficient agents, are embedded silk fibroin form soft materials. We show that these enable nongenetic activation neurons with spatial precision defined by area light illumination, neuron These CNT/silk offered reliable repeatable stimulation, 94% photoacoustic-stimulated exhibit fluorescence change larger than 10% calcium imaging light-illuminated area. The on-demand increased neurite outgrowth 1.74-fold rat dorsal root ganglion model, when compared unstimulated group. confirmed promoted is associated an concentration neurotrophic factor (BDNF). As multifunctional scaffold, demonstrated PA functions outgrowth, providing additional method nonpharmacological

Language: Английский

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Conductive Nerve Guidance Conduits Based on Morpho Butterfly Wings for Peripheral Nerve Repair

ACS Nano, Journal Year: 2022, Volume and Issue: 16(2), P. 1868 - 1879

Published: Feb. 3, 2022

Peripheral nerve injury (PNI), causing loss of sensory and motor function, is a complex challenging disease in the clinic due to restricted regeneration capacity. Nerve guidance conduits (NGCs) have become promising substitute for peripheral regeneration, but their efficacy often limited. Here, inspired by physiological structures nerves, we present conductive topological scaffold repair modifying Morpho butterfly wing with reduced graphene oxide (rGO) nanosheets methacrylated gelatin (GelMA) hydrogel encapsulated brain-derived neurotrophic factor (BDNF). Benefiting from biocompatibility GelMA hydrogel, conductivity rGO parallel nanoridge scales, PC12 cells, neural stem cells grown on modified an increased neurite length guided cellular orientation. In addition, NGCs are successfully obtained manually rolling up scaffolds exhibited great performance repairing 10 mm sciatic defects rats, believe that can be applied reparing longer future further optimization. We also demonstrate feasibility electrically based rGO/BDNF/GelMA-integrated as functional conduits, which may potential value application injuries.

Language: Английский

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Self-renewing macrophages in dorsal root ganglia contribute to promote nerve regeneration

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(7)

Published: Feb. 10, 2023

Sensory neurons located in dorsal root ganglia (DRG) convey sensory information from peripheral tissue to the brain. After nerve injury, switch a regenerative state enable axon regeneration and functional recovery. This process is not cell autonomous requires glial immune cells. Macrophages DRG (DRGMacs) accumulate response but their origin function remain unclear. Here, we mapped fate of DRGMacs injury using macrophage depletion, fate-mapping, single-cell transcriptomics. We identified three subtypes after addition small population circulating bone-marrow–derived precursors. Self-renewing macrophages, which proliferate local resident represent largest DRGMacs. The other two include microglia-like cells macrophage-like satellite (SGCs) (Imoonglia). show that self-renewing contribute promote regeneration. Using transcriptomics data CellChat simulate intercellular communication, reveal macrophages express neuroprotective glioprotective ligand prosaposin communicate with SGCs via receptor GPR37L1. These highlight have capacity self-renew, similarly microglia Central nervous system (CNS) also heterogeneity potential neuro- roles, may inform future therapeutic approaches treat injury.

Language: Английский

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Ultrasound‐Responsive Aligned Piezoelectric Nanofibers Derived Hydrogel Conduits for Peripheral Nerve Regeneration

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(28)

Published: May 15, 2024

Nerve guidance conduits (NGCs) are considered as promising treatment strategy and frontier trend for peripheral nerve regeneration, while their therapeutic outcomes limited by the lack of controllable drug delivery available physicochemical cues. Herein, novel aligned piezoelectric nanofibers derived hydrogel NGCs with ultrasound (US)-triggered electrical stimulation (ES) release repairing injury proposed. The inner layer is barium titanate nanoparticles (BTNPs)-doped polyvinylidene fluoride-trifluoroethylene [BTNPs/P(VDF-TrFE)] electrospinning improved piezoelectricity orientation. outer side thermoresponsive poly(N-isopropylacrylamide) hybrid bioactive encapsulation. Such can not only induce neuronal-oriented extension promote neurite outgrowth US-triggered wireless ES, but also realize growth factor shrinkage under heating. Thus, NGC positively accelerate functional recovery axonal regeneration rat models long sciatic defects. It believed that proposed US-responsive will find important applications in clinic neural tissue engineering.

Language: Английский

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