Comparing stem cells, transdifferentiation and brain organoids as tools for psychiatric research DOI Creative Commons
Alfredo Bellon

Translational Psychiatry, Journal Year: 2024, Volume and Issue: 14(1)

Published: Feb. 28, 2024

Abstract The inaccessibility of neurons coming directly from patients has hindered our understanding mental illnesses at the cellular level. To overcome this obstacle, six different approaches that carry genetic vulnerability to psychiatric disorders are currently available: Olfactory Neuroepithelial Cells, Mesenchymal Stem Pluripotent Monocytes, Induced Neuronal cells and more recently Brain Organoids. Here we contrast advantages disadvantages each these cell-based methodologies. Neuronal-like derived pluripotent monocytes presented in detail as technique was used psychiatry for first time. Among parameters comparison are; accessibility, need reprograming, time deliver differentiated cells, differentiation efficiency, reproducibility results cost. We provide a timeline on discovery methodologies, but, main goal is assist researchers selecting which approach best suited any given project. This manuscript also aims help readers better interpret published literature. With mind, end work with discussion about differences similarities between techniques postmortem research, only available tools allow study illness or neuronal-like patients.

Language: Английский

Harnessing the potential of hydrogels for advanced therapeutic applications: current achievements and future directions DOI Creative Commons

Peilin Lu,

Dongxue Ruan,

Meiqi Huang

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: July 1, 2024

The applications of hydrogels have expanded significantly due to their versatile, highly tunable properties and breakthroughs in biomaterial technologies. In this review, we cover the major achievements potential therapeutic applications, focusing primarily on two areas: emerging cell-based therapies promising non-cell modalities. Within context cell therapy, discuss capacity overcome existing translational challenges faced by mainstream therapy paradigms, provide a detailed discussion advantages principal design considerations for boosting efficacy as well list specific examples different disease scenarios. We then explore drug delivery, physical intervention therapies, other areas (e.g., bioadhesives, artificial tissues, biosensors), emphasizing utility beyond mere delivery vehicles. Additionally, complement our latest progress clinical application outline future research directions, particularly terms integration with advanced biomanufacturing This review aims present comprehensive view critical insights into selection both tailored meet requirements diverse diseases situations.

Language: Английский

Citations

83
Cause and consequence of heterogeneity in human mesenchymal stem cells: Challenges in clinical application DOI
Zhao Lyu,

Miaomiao Xin,

Dale Reece Oyston

et al.

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 260, P. 155354 - 155354

Published: May 15, 2024

Language: Английский

Citations

32
The Role and Prospects of Mesenchymal Stem Cells in Skin Repair and Regeneration DOI Creative Commons
Si Wu,

Shengbo Sun,

Wentao Fu

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(4), P. 743 - 743

Published: March 27, 2024

Mesenchymal stem cells (MSCs) have been recognized as a cell therapy with the potential to promote skin healing. MSCs, their multipotent differentiation ability, can generate various related wound healing, such dermal fibroblasts (DFs), endothelial cells, and keratinocytes. In addition, MSCs neovascularization, cellular regeneration, tissue healing through mechanisms including paracrine autocrine signaling. Due these characteristics, extensively studied in context of burn chronic repair. Furthermore, during investigation unique roles aging scarless also discovered. this review, we summarize by which discuss recent findings from preclinical clinical studies. We explore strategies enhance therapeutic effects MSCs. Moreover, emerging trend combining engineering techniques, leveraging advantages materials, biodegradable scaffolds hydrogels, repair capacity Additionally, highlight using characteristics cell-free therapies future direction cell-based treatments, further demonstrating regenerative aesthetic applications regeneration.

Language: Английский

Citations

23
Pluripotent stem-cell-derived therapies in clinical trial: A 2025 update DOI Creative Commons
Agnete Kirkeby,

Heather Main,

Melissa Carpenter

et al.

Cell stem cell, Journal Year: 2025, Volume and Issue: 32(1), P. 10 - 37

Published: Jan. 1, 2025

Language: Английский

Citations

12
In Vitro Engineered ECM‐incorporated Hydrogels for Osteochondral Tissue Repair: A Cell‐Free Approach DOI Creative Commons

Ali Coyle,

Aishik Chakraborty, Jiaqi Huang

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: 14(4)

Published: Jan. 5, 2025

Prevalence of osteoarthritis has been increasing in aging populations, which necessitated the use advanced biomedical treatments. These involve grafts or delivering drug molecules entrapped scaffolds. However, such treatments often show suboptimal therapeutic effects due to poor half-life and off-target molecules. As a countermeasure, 3D printable robust hydrogel-based tissue-repair platform is developed containing decellularized extracellular matrix (dECM) from differentiated mammalian cells as cargo. Here, pre-osteoblastic pre-chondrogenic murine are vitro, decellularized, incorporated into methacrylated gelatin (GelMA) solutions form osteogenic (GelO) chondrogenic (GelC) hydrogels, respectively. Integrating bioactive dECM cell sources allows GelO GelC induce differentiation human adipose-derived stem (hASCs) toward lineages. Further, can be covalently adhered using carbodiimide coupling reaction, forming multi-layered hydrogel with potential application osteochondral plug. The designed also hASCs vitro. In conclusion, carrying printed offers promising new cell-free strategy for bone cartilage repair future management.

Language: Английский

Citations

2
Recent advances to enhance the immunomodulatory potential of mesenchymal stem cells DOI Creative Commons
Madina Sarsenova,

Yevgeniy Kim,

Kamila Raziyeva

et al.

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: Sept. 23, 2022

Considering the unique therapeutic potential of mesenchymal stem cells (MSCs), including their immunosuppressive and immunomodulatory properties as well ability to improve tissue regeneration, these have attracted attention scientists clinicians for treatment different inflammatory immune system mediated disorders. However, various clinical trials using MSCs purpose are conflicting differ from results promising preclinical studies. This inconsistency is caused by several factors such poor migration homing capacities, low survival rate, level proliferation differentiation, donor-dependent variation cells. Enhancement retention persistent effects remain a challenge overcome in MSC-based therapy. In this review, we summarized approaches enhance outcomes therapy revised current future perspectives creation cellular products with improved diverse applications.

Language: Английский

Citations

68
Extracellular vesicles are key players in mesenchymal stem cells’ dual potential to regenerate and modulate the immune system DOI Creative Commons
Martijn van Griensven, Elizabeth R. Balmayor

Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 207, P. 115203 - 115203

Published: Feb. 9, 2024

MSCs are used for treatment of inflammatory conditions or regenerative purposes. complete cells and allogenic transplantation is in principle possible, but mostly autologous use preferred. In recent years, it was discovered that secrete extracellular vesicles. These active budded off vesicles carry a cargo. The cargo can be miRNA, protein, lipids etc. transported through the body fuse with target cells. Thereby, they influence phenotype modulate disease. have, like MSCs, immunomodulatory capacities. This review will focus on those features discuss their dual role. Besides immunomodulation, regeneration concentrate bone, cartilage, tendon, vessels nerves. Current clinical trials immunomodulation started last five years highlighted as well. summary, have great potential disease modulating entity treatment. Their characteristics need to taken into account often both important having best effect.

Language: Английский

Citations

14
Unraveling Adipose Tissue Dysfunction: Molecular Mechanisms, Novel Biomarkers, and Therapeutic Targets for Liver Fat Deposition DOI Creative Commons
Marta Lopez‐Yus,

C. Hörndler,

Sofía Borlán

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(5), P. 380 - 380

Published: Feb. 22, 2024

Adipose tissue (AT), once considered a mere fat storage organ, is now recognized as dynamic and complex entity crucial for regulating human physiology, including metabolic processes, energy balance, immune responses. It comprises mainly two types: white adipose (WAT) brown (BAT) thermogenesis, with beige adipocytes demonstrating the plasticity of these cells. WAT, beyond lipid storage, involved in various activities, notably lipogenesis lipolysis, critical maintaining homeostasis. also functions an endocrine secreting adipokines that influence metabolic, inflammatory, processes. However, dysfunction especially related to obesity, leads disturbances, inability properly store excess lipids, resulting ectopic deposition organs like liver, contributing non-alcoholic fatty liver disease (NAFLD). This narrative review delves into multifaceted roles its composition, functions, pathophysiology WAT dysfunction. explores diagnostic approaches adipose-related disorders, emphasizing importance accurately assessing AT distribution understanding relationships between compartments health. Furthermore, it discusses therapeutic strategies, innovative therapeutics adipose-derived mesenchymal stem cells (ADMSCs)-based treatments gene therapy, highlighting potential precision medicine targeting obesity associated complications.

Language: Английский

Citations

14
Stiff hydrogel encapsulation retains mesenchymal stem cell stemness for regenerative medicine DOI

Bo Li,

Liyuan Zhang, Yin Yuan

et al.

Matter, Journal Year: 2024, Volume and Issue: 7(10), P. 3447 - 3468

Published: June 21, 2024

Language: Английский

Citations

14
Current Strategies and Therapeutic Applications of Mesenchymal Stem Cell-Based Drug Delivery DOI Creative Commons

Yasunari Matsuzaka,

Ryu Yashiro

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(6), P. 707 - 707

Published: May 30, 2024

Mesenchymal stem cells (MSCs) have emerged as a promising approach for drug delivery strategies because of their unique properties. These include cell membrane-coated nanoparticles, cell-derived extracellular vesicles, immunomodulatory effects, cell-laden scaffolds, and scaffold-free sheets. MSCs offer advantages such low immunogenicity, homing ability, tumor tropism, making them ideal targeted systems. Stem vesicles gained attention immune properties tumor-homing abilities, presenting potential solution challenges. The relationship between MSC-based the self-renewal differentiation capabilities lies in engineered to serve effective carriers therapeutic agents while maintaining intrinsic exhibit potent immunosuppressive functions strategies. EVs immunogenicity strong tissue repair regeneration. Scaffold-free sheets represent cutting-edge regenerative medicine, offering versatile platform engineering regeneration across different medical specialties. shown great clinical applications medicine ability differentiate into various types, secrete bioactive factors, modulate responses. Researchers are exploring these innovative approaches enhance efficiency effectiveness treating diseases.

Language: Английский

Citations

13