Biomaterials, Journal Year: 2020, Volume and Issue: 268, P. 120559 - 120559
Published: Dec. 1, 2020
Language: Английский
Neuron, Journal Year: 2020, Volume and Issue: 108(1), P. 17 - 32
Published: Oct. 1, 2020
Language: Английский
Citations
178
Nature reviews. Neuroscience, Journal Year: 2022, Volume and Issue: 23(5), P. 257 - 274
Published: March 31, 2022
Language: Английский
Citations
163
Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)
Published: Nov. 17, 2021
Imaging the activity of neurons that are widely distributed across brain regions deep in scattering tissue at high speed remains challenging. Here, we introduce an open-source system with Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging (Diesel2p). Combining optical design, adaptive optics, and temporal multiplexing, offers subcellular resolution over a large ~25 mm
Language: Английский
Citations
108
Neuron, Journal Year: 2023, Volume and Issue: 111(10), P. 1547 - 1563.e9
Published: April 3, 2023
Language: Английский
Citations
75
Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)
Published: April 5, 2023
Abstract Light-based in-vivo brain imaging relies on light transport over large distances of highly scattering tissues. Scattering gradually reduces contrast and resolution, making it difficult to reach structures at greater depths even with the use multiphoton techniques. To deeper, minimally invasive endo-microscopy techniques have been established. These most commonly exploit graded-index rod lenses enable a variety modalities in head-fixed freely moving animals. A recently proposed alternative is holographic control through multimode optical fibres promising much less traumatic application superior performance. We present 110 μm thin laser-scanning endo-microscope based this prospect, enabling volumetric throughout whole depth mouse brain. The instrument equipped multi-wavelength detection three-dimensional random access options, performs lateral resolution below 1 μm. showcase various modes its observations fluorescently labelled neurones, their processes blood vessels. Finally, we demonstrate how monitor calcium signalling neurones measure flow velocity individual vessels high speeds.
Language: Английский
Citations
48
Trends in Neurosciences, Journal Year: 2023, Volume and Issue: 46(3), P. 228 - 239
Published: Jan. 10, 2023
Language: Английский
Citations
45
Journal of Neuroscience, Journal Year: 2021, Volume and Issue: 41(19), P. 4160 - 4168
Published: April 23, 2021
The brain functions through coordinated activity among distributed regions. Wide-field calcium imaging, combined with improved genetically encoded indicators, allows sufficient signal-to-noise ratio and spatiotemporal resolution to afford a unique opportunity capture cortex-wide dynamics on moment-by-moment basis in behaving animals. Recent applications of this approach have been uncovering cortical at unprecedented scales during various cognitive processes, ranging from relatively simple sensorimotor integration more complex decision-making tasks. In review, we will highlight recent scientific advances enabled by wide-field imaging mice. We then summarize several technical considerations future opportunities for uncover large-scale circuit dynamics.
Language: Английский
Citations
85
Neuroscience, Journal Year: 2022, Volume and Issue: 489, P. 15 - 33
Published: Feb. 17, 2022
Half a century since their discovery by Llinás and colleagues, dendritic spikes have been observed in various neurons different brain regions, from the neocortex cerebellum to basal ganglia. Dendrites exhibit terrifically diverse but stereotypical repertoire of spikes, sometimes specific subregions dendrite. Despite prevalence, we only glimpse into role behaving animal. This article aims survey full range found excitatory inhibitory neurons, compare themin vivoversusin vitro, discuss new studies describing human cortex. We focus on neocortical hippocampal present roadmap identify understand broader single-cell computation.
Language: Английский
Citations
70
Annual Review of Neuroscience, Journal Year: 2022, Volume and Issue: 45(1), P. 273 - 294
Published: March 22, 2022
Recent advances in fluorescence imaging permit large-scale recording of neural activity and dynamics neurochemical release with unprecedented resolution behaving animals. Calcium highly optimized genetically encoded indicators provides a mesoscopic view from defined populations at cellular subcellular resolutions. Rigorously improved voltage sensors microscopy allow for robust spike populational neurons various brain regions. In addition, recent protein engineering efforts the past few years have led to development neurotransmitters neuromodulators. Here, we discuss applications these fluorescent reporting response behaviors different biological systems as well drug discovery. We also report simple model guide sensor selection optimization.
Language: Английский
Citations
64
Science, Journal Year: 2022, Volume and Issue: 378(6619)
Published: Nov. 3, 2022
Genetically encoded fluorescent voltage indicators are ideally suited to reveal the millisecond-scale interactions among and between targeted cell populations. However, current lack requisite sensitivity for in vivo multipopulation imaging. We describe next-generation green red sensors, Ace-mNeon2 VARNAM2, their reverse response-polarity variants pAce pAceR. Our enable 0.4- 1-kilohertz recordings from >50 spiking neurons per field of view awake mice ~30-minute continuous imaging flies. Using dual-polarity multiplexed imaging, we uncovered brain state-dependent antagonism neocortical somatostatin-expressing (SST+) vasoactive intestinal peptide-expressing (VIP+) interneurons contributions hippocampal potentials ensembles with distinct axonal projections. By combining three mutually compatible indicators, performed simultaneous triple-population These approaches will empower investigations dynamic interplay neuronal subclasses at single-spike resolution.
Language: Английский
Citations
52