Synthetic and Systems Biotechnology,
Journal Year:
2025,
Volume and Issue:
10(2), P. 677 - 695
Published: March 15, 2025
DNA
storage,
characterized
by
its
durability,
data
density,
and
cost-effectiveness,
is
a
promising
solution
for
managing
the
increasing
volumes
in
healthcare.
This
review
explores
state-of-the-art
storage
technologies,
provides
insights
into
designing
system
tailored
medical
cold
data.
We
anticipate
that
practical
approach
will
involve
establishing
regional,
vitro
centers
can
serve
multiple
hospitals.
The
immediacy
of
hinges
on
development
novel,
high-density,
specialized
coding
methods.
Established
commercial
techniques,
such
as
chemical
synthesis
next-generation
sequencing
(NGS),
along
with
mixed
drying
alkaline
salts
refined
Polymerase
Chain
Reaction
(PCR),
potentially
represent
optimal
options
writing,
reading,
accessing,
respectively.
Data
security
could
be
promised
integration
traditional
digital
encryption
steganography.
Although
breakthrough
developments
like
artificial
nucleotides
nanostructures
show
potential,
they
remain
laboratory
research
phase.
In
conclusion,
viable
preservation
strategy
near
future.
Cell,
Journal Year:
2024,
Volume and Issue:
187(5), P. 1076 - 1100
Published: Feb. 1, 2024
Genome
editing
has
been
a
transformative
force
in
the
life
sciences
and
human
medicine,
offering
unprecedented
opportunities
to
dissect
complex
biological
processes
treat
underlying
causes
of
many
genetic
diseases.
CRISPR-based
technologies,
with
their
remarkable
efficiency
easy
programmability,
stand
at
forefront
this
revolution.
In
Review,
we
discuss
current
state
CRISPR
gene
technologies
both
research
therapy,
highlighting
limitations
that
constrain
them
technological
innovations
have
developed
recent
years
address
them.
Additionally,
examine
summarize
landscape
applications
context
health
therapeutics.
Finally,
outline
potential
future
developments
could
shape
coming
years.
Nature Biotechnology,
Journal Year:
2023,
Volume and Issue:
41(10), P. 1446 - 1456
Published: Feb. 16, 2023
Most
short
sequences
can
be
precisely
written
into
a
selected
genomic
target
using
prime
editing;
however,
it
remains
unclear
what
factors
govern
insertion.
We
design
library
of
3,604
various
lengths
and
measure
the
frequency
their
insertion
four
sites
in
three
human
cell
lines,
different
editor
systems
varying
DNA
repair
contexts.
find
that
length,
nucleotide
composition
secondary
structure
sequence
all
affect
rates.
also
discover
3'
flap
nucleases
TREX1
TREX2
suppress
longer
sequences.
Combining
features
machine
learning
model,
we
predict
relative
insertions
site
with
R
=
0.70.
Finally,
demonstrate
how
our
accurate
prediction
user-friendly
software
help
choose
codon
variants
common
fusion
tags
insert
at
high
efficiency,
provide
catalog
empirically
determined
rates
for
over
hundred
useful
Cell,
Journal Year:
2024,
Volume and Issue:
187(10), P. 2411 - 2427.e25
Published: April 11, 2024
We
set
out
to
exhaustively
characterize
the
impact
of
cis-chromatin
environment
on
prime
editing,
a
precise
genome
engineering
tool.
Using
highly
sensitive
method
for
mapping
genomic
locations
randomly
integrated
reporters,
we
discover
massive
position
effects,
exemplified
by
editing
efficiencies
ranging
from
∼0%
94%
an
identical
target
site
and
edit.
Position
effects
efficiency
are
well
predicted
chromatin
marks,
e.g.,
positively
H3K79me2
negatively
H3K9me3.
Next,
developed
multiplex
perturbational
framework
assess
interaction
trans-acting
factors
with
outcomes.
Applying
this
DNA
repair
factors,
identify
HLTF
as
context-dependent
repressor
editing.
Finally,
several
lines
evidence
suggest
that
active
transcriptional
elongation
enhances
Consistent
this,
show
can
robustly
decrease
or
increase
preceding
it
CRISPR-mediated
silencing
activation,
respectively.
