Developing high-affinity, oxygen-insensitive [NiFe]-hydrogenases as biocatalysts for energy conversion DOI Creative Commons
Chris Greening, Ashleigh Kropp, Kylie A. Vincent

et al.

Biochemical Society Transactions, Journal Year: 2023, Volume and Issue: 51(5), P. 1921 - 1933

Published: Sept. 25, 2023

The splitting of hydrogen (H2) is an energy-yielding process, which important for both biological systems and as a means providing green energy. In biology, this reaction mediated by enzymes called hydrogenases, utilise complex nickel iron cofactors to split H2 transfer the resulting electrons electron-acceptor. These [NiFe]-hydrogenases have received considerable attention catalysts in fuel cells, produce electrical current. are promising alternative platinum-based that currently predominate cells due abundance iron, resistance some family members inhibition gases, including carbon monoxide, rapidly poison catalysts. However, majority characterised inhibited oxygen (O2), limiting their activity stability. We recently reported isolation characterisation [NiFe]-hydrogenase Huc from Mycobacterium smegmatis, insensitive O2 has extremely high affinity, making it capable oxidising air below atmospheric concentrations. properties make candidate development enzyme-based (EBFCs), at low concentrations impure gas mixtures. review, we aim provide context use purpose discussing advantages deployment cells. also address challenges associated with using purpose, how these might be overcome develop EBFCs can deployed scale.

Language: Английский

Nature-inspired interfacial engineering for energy harvesting DOI
Baoping Zhang, Wanghuai Xu, Liang Peng

et al.

Nature Reviews Electrical Engineering, Journal Year: 2024, Volume and Issue: 1(4), P. 218 - 233

Published: March 18, 2024

Language: Английский

Citations

37
The impact of AI‐based modeling on the accuracy of protein assembly prediction: Insights from CASP15 DOI Creative Commons
Burcu Çelet Özden,

Andriy Kryshtafovych,

Ezgi Karaca

et al.

Proteins Structure Function and Bioinformatics, Journal Year: 2023, Volume and Issue: 91(12), P. 1636 - 1657

Published: Oct. 20, 2023

In CASP15, 87 predictors submitted around 11 000 models on 41 assembly targets. The community demonstrated exceptional performance in overall fold and interface contact predictions, achieving an impressive success rate of 90% (compared to 31% CASP14). This remarkable accomplishment is largely due the incorporation DeepMind's AF2-Multimer approach into custom-built prediction pipelines. To evaluate added value participating methods, we compared baseline predictor. over 1/3 cases, were superior main reasons for this improved use multiple sequence alignments, optimized sampling, manual AF2-Multimer-built subcomplexes. best three groups, order, are Zheng, Venclovas, Wallner. Zheng Venclovas reached a 73.2% all (41) while Wallner attained 69.4% 36 cases. Nonetheless, challenges remain predicting structures with weak evolutionary signals, such as nanobody-antigen, antibody-antigen, viral complexes. Expectedly, modeling large complexes also remains challenging their high memory compute demands. addition category, assessed accuracy interdomain interfaces tertiary structure Models seven targets featuring 17 unique analyzed. Best achieved 76.5% rate, UM-TBM group being leader. observed that faced challenges, case when signal given domain pair was or large. Overall, CASP15 witnessed unprecedented improvement modeling, reflecting AI revolution seen CASP14.

Language: Английский

Citations

25
Protein target highlights in CASP15: Analysis of models by structure providers DOI Creative Commons
Leila T. Alexander,

Janani Durairaj,

Andriy Kryshtafovych

et al.

Proteins Structure Function and Bioinformatics, Journal Year: 2023, Volume and Issue: 91(12), P. 1571 - 1599

Published: July 26, 2023

We present an in-depth analysis of selected CASP15 targets, focusing on their biological and functional significance. The authors the structures identify discuss key protein features evaluate how effectively these aspects were captured in submitted predictions. While overall ability to predict three-dimensional continues impress, reproducing uncommon not previously observed experimental is still a challenge. Furthermore, instances with conformational flexibility large multimeric complexes highlight need for novel scoring strategies better emphasize biologically relevant structural regions. Looking ahead, closer integration computational techniques will play role determining next challenges be unraveled field molecular biology.

Language: Английский

Citations

24
Hydrogen sulfide donors across time: From origins to cutting-edge applications DOI

Yuanqin Zhao,

Yanxia Wang, Qian Xu

et al.

Nitric Oxide, Journal Year: 2024, Volume and Issue: 144, P. 29 - 39

Published: Feb. 1, 2024

Language: Английский

Citations

11
Remarkably enhanced molecular sieving effect of carbon molecular sieve membrane by enhancing the concentration of thermally rearranged precursors DOI

Mingwei Cai,

Jiongcai Chen,

Heng Liu

et al.

Separation and Purification Technology, Journal Year: 2024, Volume and Issue: 341, P. 126945 - 126945

Published: Feb. 29, 2024

Language: Английский

Citations

11
Outer-sphere effects on the O2 sensitivity, catalytic bias and catalytic reversibility of hydrogenases DOI Creative Commons
Andrea Fasano, Vincent Fourmond, Christophe Léger

et al.

Chemical Science, Journal Year: 2024, Volume and Issue: 15(15), P. 5418 - 5433

Published: Jan. 1, 2024

The comparison of homologous hydrogenases, in which the same inorganic active site is surrounded by a variable protein matrix, has demonstrated that residues are remote from may have great influence on catalytic properties.

Language: Английский

Citations

8
Trace gas oxidation sustains energy needs of a thermophilic archaeon at suboptimal temperatures DOI Creative Commons
Pok Man Leung, Rhys Grinter, Eve Tudor-Matthew

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 15, 2024

Abstract Diverse aerobic bacteria use atmospheric hydrogen (H 2 ) and carbon monoxide (CO) as energy sources to support growth survival. Such trace gas oxidation is recognised a globally significant process that serves the main sink in biogeochemical H cycle sustains microbial biodiversity oligotrophic ecosystems. However, it unclear whether archaea can also . Here we show thermoacidophilic archaeon, Acidianus brierleyi (Thermoproteota), constitutively consumes CO sub-atmospheric levels. Oxidation occurs across wide range of temperatures (10 70 °C) enhances ATP production during starvation-induced persistence under temperate conditions. The genome A. encodes canonical dehydrogenase four distinct [NiFe]-hydrogenases, which are differentially produced response electron donor acceptor availability. Another Metallosphaera sedula , oxidize Our results suggest common trait Sulfolobales may play role their survival niche expansion, including dispersal through environments.

Language: Английский

Citations

5
Dynamic Stereo‐Conformation of Catalyst‐In‐Cavity Biomimetic Enzymes Enable High‐Sulfur‐Utilization and Lean‐Electrolyte Lithium‐Sulfur Batteries DOI
Yang‐Yang Dong, Haikuo Zhang, Dong Cai

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: June 14, 2024

Abstract The key challenges for pursuing high‐energy‐density lithium‐sulfur (Li‐S) batteries lie in struggling sulfur conversions under high‐sulfur‐loading and low electrolyte‐to‐sulfur (E/S) ratio conditions. To mimic high‐efficient natural enzymes, this work, sulfonated cobalt phthalocyanine (CoPC) is implanted β‐cyclodextrin (β–CD) cavity preparing β–CD@CoPC biomimetic catalysts, which enables a good electrochemical contact with solvated lithium polysulfides locally enriched electrolyte. Moreover, the dynamic stereo‐conformation evolution of CoPC hydrophobic β–CD promotes subsequent redox kinetics induces separation Li 2 S from catalyst, further improving catalysis efficiency. catalytic activity quantified through specific Michaelis–Menten equations enzymatic reactions it showed that improved ability by 3 30 times compared to respectively. As result, Li–S pouch cell mere 1.0 wt% exhibits high initial areal capacity 11.6 mAh cm −2 (12.8 mg ) E/S (3.5 µL −1 ). This strategy provides feasible way tackle conversion issues lean electrolytes next‐generation high‐energy batteries.

Language: Английский

Citations

5
Structure of a fully assembled γδ T-cell antigen receptor DOI Creative Commons
Benjamin S. Gully, João Ferreira Fernandes, Sachith D. Gunasinghe

et al.

Nature, Journal Year: 2024, Volume and Issue: 634(8034), P. 729 - 736

Published: Aug. 15, 2024

T cells in jawed vertebrates comprise two lineages, αβ and γδ cells, defined by the antigen receptors they express-that is, cell (TCRs), respectively. The lineages have different immunological roles, requiring that TCRs recognize more structurally diverse ligands

Language: Английский

Citations

4
High-resolution cryo-EM using a common LaB 6 120-keV electron microscope equipped with a sub–200-keV direct electron detector DOI Creative Commons
Hariprasad Venugopal, Jesse I. Mobbs, Cyntia Taveneau

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(1)

Published: Jan. 3, 2025

High-resolution cryo–electron microscopy (cryo-EM) requires costly 200- to 300-keV cryo–transmission electron microscopes (cryo-TEMs) with field emission gun (FEG) sources, stable columns, constant-powered lenses, autoloader, and direct detectors (DED). Recent advances in 100-keV imaging the emergence of sub–200-keV optimized DED technology promises development more affordable cryo-TEMs. So far, has required FEG sources. We here explored whether a standard 120-keV TEMs thermionic lanthanum hexaboride (LaB 6 ) source can be upgraded for high-resolution cryo-EM. Using this configuration, we successfully obtained 2.65 Å reconstruction apoferritin, 4.33 64-kDa hemoglobin, 4.4 an asymmetric 153kDa membrane protein GPCR. All results were achieved using automated data collection SerialEM, demonstrating feasibility collect large cryo-EM datasets side-entry cryo-holder. These showcase widely accessible solution obtaining interpretable structures at low cost contribute “democratization”

Language: Английский

Citations

0