Covalent inhibition of epidermal growth factor receptor using a long-lived iridium(III)-afatinib probe DOI

Sang-Cuo Nao,

Lingtan Kong,

Daniel Shiu‐Hin Chan

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 259, P. 129211 - 129211

Published: Jan. 4, 2024

Language: Английский

μMap-Red: Proximity Labeling by Red Light Photocatalysis DOI

Benito F. Buksh,

Steve D. Knutson, James V. Oakley

et al.

Journal of the American Chemical Society, Journal Year: 2022, Volume and Issue: 144(14), P. 6154 - 6162

Published: April 1, 2022

Modern proximity labeling techniques have enabled significant advances in understanding biomolecular interactions. However, current tools primarily utilize activation modes that are incompatible with complex biological environments, limiting our ability to interrogate cell- and tissue-level microenvironments animal models. Here, we report μMap-Red, a platform uses red-light-excited Sn

Language: Английский

Citations

78
Visible Light-Induced Reactions of Diazo Compounds and Their Precursors DOI
Ziyan Zhang, Vladimir Gevorgyan

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(11), P. 7214 - 7261

Published: May 16, 2024

In recent years, visible light-induced reactions of diazo compounds have attracted increasing attention in organic synthesis, leading to improvement existing reactions, as well the discovery unprecedented transformations. Thus, photochemical or photocatalytic generation both carbenes and radicals provide milder tools toward these key intermediates for many valuable However, vast majority transformations represent new reactivity modes compounds, which are achieved by decomposition photoredox catalysis. particular, use a redox-active photocatalysts opens avenue plethora radical reactions. The application methods led inaccessible classical associated with metal carbenes. most cases, act sources but can also serve acceptors. Importantly, described processes operate under mild, practical conditions. This Review describes this subfield compound chemistry, particularly focusing on advancements.

Language: Английский

Citations

67
Lamins: The backbone of the nucleocytoskeleton interface DOI Creative Commons

Joan M. Sobo,

Nicholas S. Alagna,

Sean X. Sun

et al.

Current Opinion in Cell Biology, Journal Year: 2024, Volume and Issue: 86, P. 102313 - 102313

Published: Jan. 22, 2024

Language: Английский

Citations

23
Bioorthogonal photocatalytic proximity labeling in primary living samples DOI Creative Commons
Ziqi Liu,

Fuhu Guo,

Yufan Zhu

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 28, 2024

Abstract In situ profiling of subcellular proteomics in primary living systems, such as native tissues or clinic samples, is crucial for understanding life processes and diseases, yet challenging due to methodological obstacles. Here we report CAT-S, a bioorthogonal photocatalytic chemistry-enabled proximity labeling method, that expands wide range samples mitochondrial proteomes. Powered by our thioQM warhead development targeted chemistry, CAT-S enables the proteins cells with high efficiency specificity. We apply diverse cell cultures, dissociated mouse well T from human blood, portraying native-state proteomic characteristics, unveiled hidden (PTPN1, SLC35A4 uORF, TRABD). Furthermore, allows quantification perturbations on dysfunctional tissues, exampled diabetic kidneys, revealing alterations lipid metabolism may drive disease progression. Given advantages non-genetic operation, generality, spatiotemporal resolution, open exciting avenues investigations are otherwise inaccessible.

Language: Английский

Citations

21
Discovery and significance of protein-protein interactions in health and disease DOI Creative Commons
Jack Greenblatt, Bruce Alberts, Nevan J. Krogan

et al.

Cell, Journal Year: 2024, Volume and Issue: 187(23), P. 6501 - 6517

Published: Nov. 1, 2024

The identification of individual protein-protein interactions (PPIs) began more than 40 years ago, using protein affinity chromatography and antibody co-immunoprecipitation. As new technologies emerged, analysis PPIs increased to a genome-wide scale with the introduction intracellular tagging methods, purification (AP) followed by mass spectrometry (MS), co-fractionation MS (CF-MS). Now, combining resulting catalogs complementary including crosslinking (XL-MS) cryogenic electron microscopy (cryo-EM), helps distinguish direct from indirect ones within same or between different complexes. These powerful approaches promise artificial intelligence applications like AlphaFold herald future where complexes, energy-driven machines, will be understood in exquisite detail, unlocking insights contexts both basic biology disease.

Language: Английский

Citations

19
A genetically encoded photoproximity labeling approach for mapping protein territories DOI Creative Commons
Nir Hananya, Xuanjia Ye, Shany Koren

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(16)

Published: April 10, 2023

Studying dynamic biological processes requires approaches compatible with the lifetimes of biochemical transactions under investigation, which can be very short. We describe a genetically encoded system that allows protein neighborhoods to mapped using visible light. Our approach involves fusing an engineered flavoprotein interest. Brief excitation fusion leads labeling nearby proteins cell-permeable probes. Mechanistic studies reveal different pathways are operational depending on nature exogenous probe is employed. When combined quantitative proteomics, this photoproximity generates “snapshots” territories high temporal and spatial resolution. The intrinsic fluorescence domain permits correlated imaging proteomics analyses, capability exploited in several contexts, including defining clients major vault protein. technology should broadly useful biomedical area.

Language: Английский

Citations

34
Small-molecule probes from bench to bedside: advancing molecular analysis of drug–target interactions toward precision medicine DOI
Sijun Pan, Aixiang Ding, Yisi Li

et al.

Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 52(16), P. 5706 - 5743

Published: Jan. 1, 2023

Integrated development of designer chemical tools and complementary analytical technologies empowers new scientific clinical opportunities.

Language: Английский

Citations

33
Lutetium texaphyrin: A photocatalyst that triggers pyroptosis via biomolecular photoredox catalysis DOI Creative Commons
Yunjie Xu, Calvin V. Chau, Jieun Lee

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(9)

Published: Feb. 21, 2024

Photon-controlled pyroptosis activation (PhotoPyro) is a promising technique for cancer immunotherapy due to its noninvasive nature, precise control, and ease of operation. Here, we report that biomolecular photoredox catalysis in cells might be an important mechanism underlying PhotoPyro. Our findings reveal the photocatalyst lutetium texaphyrin (

Language: Английский

Citations

18
Current advances in photocatalytic proximity labeling DOI
Steve D. Knutson,

Benito F. Buksh,

Sean W. Huth

et al.

Cell chemical biology, Journal Year: 2024, Volume and Issue: 31(6), P. 1145 - 1161

Published: April 24, 2024

Language: Английский

Citations

17
µMap proximity labeling in living cells reveals stress granule disassembly mechanisms DOI
Chenmengxiao Pan, Steve D. Knutson, Sean W. Huth

et al.

Nature Chemical Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

Language: Английский

Citations

13