Advances and applications in single-cell and spatial genomics DOI
Jingjing Wang, Fang Ye, Haoxi Chai

et al.

Science China Life Sciences, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 20, 2024

Language: Английский

Interferon-responsive intestinal BEST4/CA7+ cells are targets of bacterial diarrheal toxins DOI Creative Commons

Daisong Wang,

Willem Kasper Spoelstra, Lin Lin

et al.

Cell stem cell, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

BEST4/CA7+ cells of the human intestine were recently identified by single-cell RNA sequencing. While their gene expression profile predicts a role in electrolyte balance, cell function has not been explored experimentally owing to absence mice and paucity vitro models. Here, we establish protocol that allows emergence intestinal organoids. Differentiation requires activation Notch signaling transcription factor SPIB. numbers strongly increase response cytokine interferon-γ, supporting immunity. Indeed, demonstrate generate robust CFTR-mediated fluid efflux when stimulated with bacterial diarrhea-causing toxins find norepinephrine-ADRA2A axis as potential mechanism blocking cell-mediated secretion. Our observations identify central homeostasis infections.

Language: Английский

Citations

1
Human BioMolecular Atlas Program (HuBMAP): 3D Human Reference Atlas construction and usage DOI Creative Commons
Katy Börner, Philip D. Blood, Jonathan C. Silverstein

et al.

Nature Methods, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Abstract The Human BioMolecular Atlas Program (HuBMAP) aims to construct a 3D Reference (HRA) of the healthy adult body. Experts from 20+ consortia collaborate develop Common Coordinate Framework (CCF), knowledge graphs and tools that describe multiscale structure human body (from organs tissues down cells, genes biomarkers) use HRA characterize changes occur with aging, disease other perturbations. v.2.0 covers 4,499 unique anatomical structures, 1,195 cell types 2,089 biomarkers (such as genes, proteins lipids) 33 ASCT+B tables 65 Objects linked ontologies. New experimental data can be mapped into using (1) type annotation (for example, Azimuth), (2) validated antibody panels or (3) by registering tissue spatially. This paper describes user stories, terminology, formats, ontology validation, unified analysis workflows, interfaces, instructional materials, application programming flexible hybrid cloud infrastructure previews atlas usage applications.

Language: Английский

Citations

1
Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation DOI
Pratik Singh, Wei Yong Gu, Shariq Madha

et al.

Cell stem cell, Journal Year: 2024, Volume and Issue: 31(7), P. 1038 - 1057.e11

Published: May 10, 2024

Language: Английский

Citations

7
Intestinal organ chips for disease modelling and personalized medicine DOI
Aliçan Özkan, Nina LoGrande, Jéssica F. Feitor

et al.

Nature Reviews Gastroenterology & Hepatology, Journal Year: 2024, Volume and Issue: 21(11), P. 751 - 773

Published: Aug. 27, 2024

Language: Английский

Citations

7
Advances and applications in single-cell and spatial genomics DOI
Jingjing Wang, Fang Ye, Haoxi Chai

et al.

Science China Life Sciences, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 20, 2024

Language: Английский

Citations

7