Mapping the developmental profile of ventricular zone–derived neurons in the human cerebellum DOI Creative Commons
Anders W. Erickson,

Haodan Tan,

Liam D. Hendrikse

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(17)

Published: April 18, 2025

The cerebellar ventricular zone (VZ) is the primary source of progenitors that generate GABAergic neurons, including Purkinje cells (PCs) and interneurons (INs). This study provides detailed characterization human neurogenesis using transcriptomic histopathological analyses reveals conserved unique features compared to rodents. We show sequential progression occurs before 8 postconception weeks. Notably, PC differentiation in outer subventricular (SVZ), a region absent mouse cerebellum. Human PCs are generated during compact two-week period onset cerebral cortex histogenesis. A subset retain proliferative marker expression weeks after leaving VZ, another feature not observed maturation protracted with an extensive migration reorganization throughout development dendritic arborization developing late gestation. define continuous transcriptional cascade from neuroepithelial mature PCs. In contrast, while interneuronal born beginning early fetal development, they exhibit even more across gestation into postnatal ages. These findings dynamic developmental process for neurons underscore importance embryonic environment, disruptions having potentially significant impacts.

Language: Английский

Sex-biased gene expression across mammalian organ development and evolution DOI

Leticia Rodríguez-Montes,

Svetlana Ovchinnikova, Xuefei Yuan

et al.

Science, Journal Year: 2023, Volume and Issue: 382(6670)

Published: Nov. 2, 2023

Sexually dimorphic traits are common among mammals and specified during development through the deployment of sex-specific genetic programs. Because little is known about these programs, we investigated them using a resource gene expression profiles in males females throughout five organs (human, mouse, rat, rabbit, opossum) bird (chicken). We found that sex-biased varied considerably across species was often cell-type specific. Sex differences increased abruptly around sexual maturity instead increasing gradually organ development. Finally, evolved rapidly at level, with between evolutionary mechanisms used, but more slowly cellular same cell types being sexually species.

Language: Английский

Citations

45
Human cerebellar organoids with functional Purkinje cells DOI Creative Commons
Alexander Atamian, Marcella Birtele, Negar Hosseini

et al.

Cell stem cell, Journal Year: 2024, Volume and Issue: 31(1), P. 39 - 51.e6

Published: Jan. 1, 2024

Language: Английский

Citations

39
Developmental origins and evolution of pallial cell types and structures in birds DOI
Bastienne Zaremba, Amir Fallahshahroudi, Céline Schneider

et al.

Science, Journal Year: 2025, Volume and Issue: 387(6735)

Published: Jan. 2, 2025

Innovations in the pallium likely facilitated evolution of advanced cognitive abilities birds. We therefore scrutinized its cellular composition and using cell type atlases from chicken, mouse, nonavian reptiles. found that avian shares most inhibitory neuron types with other amniotes. Whereas excitatory amniote hippocampal regions show evolutionary conservation, those pallial have diverged. Neurons mesopallium display gene expression profiles akin to mammalian claustrum deep cortical layers, while certain nidopallial resemble neurons piriform cortex. Lastly, we observed substantial convergence between dorsally located hyperpallium ventrally nidopallium during late development, suggesting topological location does not always dictate programs determining functional properties adult pallium.

Language: Английский

Citations

3
Benchmark of cellular deconvolution methods using a multi-assay dataset from postmortem human prefrontal cortex DOI Creative Commons
Louise A. Huuki-Myers, Kelsey D. Montgomery, Sang Ho Kwon

et al.

Genome biology, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 7, 2025

Language: Английский

Citations

3
Lifelong restructuring of 3D genome architecture in cerebellar granule cells DOI
Longzhi Tan, Jenny Shi, Siavash Moghadami

et al.

Science, Journal Year: 2023, Volume and Issue: 381(6662), P. 1112 - 1119

Published: Sept. 7, 2023

The cerebellum contains most of the neurons in human brain and exhibits distinctive modes development aging. In this work, by developing our single-cell three-dimensional (3D) genome assay—diploid chromosome conformation capture, or Dip-C—into population-scale (Pop-C) virus-enriched (vDip-C) modes, we resolved first 3D structures single cerebellar cells, created life-spanning atlases for both humans mice, jointly measured transcriptome chromatin accessibility during development. We found that although granule mature early postnatal life, architecture gradually remodels throughout establishing ultra–long-range intrachromosomal contacts specific interchromosomal are rarely seen neurons. These results reveal unexpected evolutionarily conserved molecular processes underlie features neural aging across mammalian life span.

Language: Английский

Citations

42
Custom microfluidic chip design enables cost-effective three-dimensional spatiotemporal transcriptomics with a wide field of view DOI Creative Commons
Junjie Zhu, Kun Pang, Beiyu Hu

et al.

Nature Genetics, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Language: Английский

Citations

12
New clues for the role of cerebellum in schizophrenia and the associated cognitive impairment DOI Creative Commons
Pawan Faris, Doris Pischedda, Fulvia Palesi

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: May 10, 2024

Schizophrenia (SZ) is a complex neuropsychiatric disorder associated with severe cognitive dysfunction. Although research has mainly focused on forebrain abnormalities, emerging results support the involvement of cerebellum in SZ physiopathology, particularly Cognitive Impairment Associated (CIAS). Besides its role motor learning and control, implicated cognition emotion. Recent suggests that structural functional changes are linked to deficits various domains including attention, working memory, decision-making. Moreover, cerebellar dysfunction related altered circuit activities connectivity brain regions processing. This review delves into CIAS. We initially consider major alterations CIAS, addressing impairments neurotransmitter systems, synaptic plasticity, connectivity. then focus recent findings showing several mechanisms also communication impaired. evidence implicates as key component circuits underpinning CIAS physiopathology. Further studies warranted might open new perspectives toward understanding physiopathology effective treatment these disorders.

Language: Английский

Citations

11
Compartments in medulloblastoma with extensive nodularity are connected through differentiation along the granular precursor lineage DOI Creative Commons
David R. Ghasemi, Konstantin Okonechnikov, Anne Rademacher

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 8, 2024

Medulloblastomas with extensive nodularity are cerebellar tumors characterized by two distinct compartments and variable disease progression. The mechanisms governing the balance between proliferation differentiation in MBEN remain poorly understood. Here, we employ a multi-modal single cell transcriptome analysis to dissect this process. In internodular compartment, identify proliferating granular neuronal precursor-like malignant cells, along stromal, vascular, immune cells. contrast, nodular compartment comprises postmitotic, neuronally differentiated Both connected through an intermediate stage resembling actively migrating CGNPs. Notably, also discover astrocytic-like found proximity cells at transition zone compartments. Our study sheds light on spatial tissue organization its link developmental trajectory, resulting more benign tumor phenotype. This integrative approach holds promise explore intercompartmental interactions other cancers varying histology.

Language: Английский

Citations

10
MAP4 kinase-regulated reduced CLSTN1 expression in medulloblastoma is associated with increased invasiveness DOI Creative Commons

Ece Sönmez,

Yan Shen, Meng-Syuan Lin

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 6, 2025

Abstract De-regulated protein expression contributes to tumor growth and progression in medulloblastoma (MB), the most common malignant brain children. MB is associated with impaired differentiation of specific neural progenitors, suggesting that deregulation proteins involved physiology could contribute transformed phenotype MB. Calsynthenin 1 (CLSTN1) a neuronal cell-cell interaction, vesicle trafficking, synaptic signaling. We previously identified CLSTN1 as putative target pro-invasive kinase MAP4K4, which we found reduce surface expression. Herein, explored functional significance decreased primary tumors compared tumor-free cerebellum or tissues. expressed laboratory-established cell lines, where it localized plasma membrane, intracellular vesicular structures, regions contact. The reduction significantly increased factor-driven invasiveness. Pharmacological inhibition pro-migratory MAP4 kinases caused accumulation contacts. Co-culture cells astrocytes localization contacts, was further enhanced by MAP4K inhibition. Our study revealed repressive function growth-factor-driven invasiveness MB, repressors recruitment points towards implication kinase-controlled regulation tumor-microenvironment interaction.

Language: Английский

Citations

1
A single-nucleus RNA sequencing atlas of the postnatal retina of the shark Scyliorhinus canicula DOI Creative Commons
Nicolás Vidal-Vázquez, Ismael Hernández‐Núñez, Pablo Carballo-Pacoret

et al.

Scientific Data, Journal Year: 2025, Volume and Issue: 12(1)

Published: Feb. 7, 2025

Language: Английский

Citations

1