Journal of Neuroscience,
Journal Year:
2021,
Volume and Issue:
41(5), P. 845 - 854
Published: Jan. 20, 2021
Spinal
interneurons
are
important
facilitators
and
modulators
of
motor,
sensory,
autonomic
functions
in
the
intact
CNS.
This
heterogeneous
population
neurons
is
now
widely
appreciated
to
be
a
key
component
plasticity
recovery.
review
highlights
our
current
understanding
spinal
interneuron
heterogeneity,
their
contribution
control
modulation
motor
sensory
functions,
how
this
role
might
change
after
traumatic
cord
injury.
We
also
offer
perspective
for
treatments
can
optimize
functional
improvement.
Cell,
Journal Year:
2024,
Volume and Issue:
187(10), P. 2343 - 2358
Published: May 1, 2024
As
the
number
of
single-cell
datasets
continues
to
grow
rapidly,
workflows
that
map
new
data
well-curated
reference
atlases
offer
enormous
promise
for
biological
community.
In
this
perspective,
we
discuss
key
computational
challenges
and
opportunities
reference-mapping
algorithms.
We
how
mapping
algorithms
will
enable
integration
diverse
across
disease
states,
molecular
modalities,
genetic
perturbations,
species
eventually
replace
manual
laborious
unsupervised
clustering
pipelines.
Neuron,
Journal Year:
2024,
Volume and Issue:
112(15), P. 2524 - 2539.e5
Published: June 5, 2024
Altered
transcriptional
and
epigenetic
regulation
of
brain
cell
types
may
contribute
to
cognitive
changes
with
advanced
age.
Using
single-nucleus
multi-omic
DNA
methylation
transcriptome
sequencing
(snmCT-seq)
in
frontal
cortex
from
young
adult
aged
donors,
we
found
widespread
age-
sex-related
variation
specific
neuron
types.
The
proportion
inhibitory
SST-
VIP-expressing
neurons
was
reduced
donors.
Excitatory
had
more
profound
age-related
their
gene
expression
than
cells.
Hundreds
genes
involved
synaptic
activity,
including
EGR1,
were
less
expressed
adults.
Genes
located
subtelomeric
regions
increased
age
correlated
telomere
length.
We
further
mapped
cell-type-specific
sex
differences
X-inactivation
escape
genes.
Multi-omic
epigenomes
transcriptomes
provide
new
insight
into
the
effects
on
human
neurons.
Nature,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Biological
ageing
can
be
defined
as
a
gradual
loss
of
homeostasis
across
various
aspects
molecular
and
cellular
function1,2.
Mammalian
brains
consist
thousands
cell
types3,
which
may
differentially
susceptible
or
resilient
to
ageing.
Here
we
present
comprehensive
single-cell
RNA
sequencing
dataset
containing
roughly
1.2
million
high-quality
transcriptomes
brain
cells
from
young
adult
aged
mice
both
sexes,
regions
spanning
the
forebrain,
midbrain
hindbrain.
High-resolution
clustering
all
results
in
847
clusters
reveals
at
least
14
age-biased
that
are
mostly
glial
types.
At
broader
subclass
supertype
levels,
find
age-associated
gene
expression
signatures
provide
list
2,449
unique
expressed
genes
(age-DE
genes)
for
many
neuronal
non-neuronal
Whereas
most
age-DE
specific
types,
observe
common
with
including
decrease
related
structure
function
neuron
major
astrocyte
types
mature
oligodendrocytes,
an
increase
immune
function,
antigen
presentation,
inflammation,
motility
some
vascular
Finally,
demonstrate
greatest
sensitivity
concentrated
around
third
ventricle
hypothalamus,
tanycytes,
ependymal
cells,
certain
arcuate
nucleus,
dorsomedial
nucleus
paraventricular
express
canonically
energy
homeostasis.
Many
these
response.
These
findings
suggest
hypothalamus
hub
mouse
brain.
Overall,
this
study
systematically
delineates
dynamic
landscape
cell-type-specific
transcriptomic
changes
associated
normal
will
serve
foundation
investigation
functional
interaction
disease.
A
inform
into
Nature Genetics,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 3, 2025
Abstract
Identifying
gene
expression
differences
in
heterogeneous
tissues
across
conditions
is
a
fundamental
biological
task,
enabled
by
multi-condition
single-cell
RNA
sequencing
(RNA-seq).
Current
data
analysis
approaches
divide
the
constituent
cells
into
clusters
meant
to
represent
cell
types,
but
such
discrete
categorization
tends
be
an
unsatisfactory
model
of
underlying
biology.
Here,
we
introduce
latent
embedding
multivariate
regression
(LEMUR),
that
operates
without,
or
before,
commitment
categorization.
LEMUR
(1)
integrates
from
different
conditions,
(2)
predicts
each
cell’s
changes
as
function
and
its
position
space
(3)
for
gene,
identifies
compact
neighborhood
with
consistent
differential
expression.
We
apply
cancer,
zebrafish
development
spatial
gradients
Alzheimer’s
disease,
demonstrating
broad
applicability.
Cell Reports Medicine,
Journal Year:
2025,
Volume and Issue:
unknown, P. 101920 - 101920
Published: Jan. 1, 2025
Metabolic
reprogramming
of
tumor
cells
is
an
emerging
hallmark
cancer.
Among
all
the
changes
in
cancer
metabolism,
increased
glucose
uptake
and
accumulation
lactate
under
normoxic
conditions
(the
"Warburg
effect")
a
common
feature
cells.
In
this
study,
we
develop
lactate-responsive
drug
delivery
platform
by
targeting
Warburg
effect.
We
design
test
gold/mesoporous
silica
Janus
nanoparticle
system
as
gated
carrier,
which
gold
particles
are
functionalized
with
oxidase
capped
α-cyclodextrin
through
surface
arylboronate
modification.
presence
lactate,
generates
hydrogen
peroxide,
induces
self-immolation
reaction
arylboronate,
leading
to
uncapping
release.
Our
results
demonstrate
greatly
improved
specificity
therapeutic
efficacy
for
treatment
different
cancers.
findings
present
effective
approach
metabolic
tumors.
Cell Genomics,
Journal Year:
2025,
Volume and Issue:
unknown, P. 100768 - 100768
Published: Feb. 1, 2025
Emerging
research
underscores
the
gut
microbiome's
impact
on
nervous
system
via
microbiota-gut-brain
axis,
yet
comprehensive
insights
remain
limited.
Using
a
CHD8-haploinsufficient
model
for
autism
spectrum
disorder
(ASD),
we
explored
host-gut
microbiota
interactions
by
constructing
single-cell
transcriptome
atlas
of
brain
and
intestinal
tissues
in
wild-type
mutant
mice
across
three
developmental
stages.
CHD8
haploinsufficiency
caused
delayed
development
radial
glial
precursors
excitatory
neural
progenitors
E14.5
brain,
inflammation
adult
immunodeficiency,
abnormal
development.
Selective
knockdown
epithelial
cells
generated
Chd8ΔIEC
mice,
which
exhibited
normal
sociability
but
impaired
social
novelty
recognition.
Probiotic
intervention
with
Lactobacillus
murinus
selectively
rescued
deficits
analysis
revealing
underlying
mechanisms.
This
study
provides
detailed
transcriptomic
dataset
ASD-related
changes,
advancing
our
understanding
gut-brain
axis
offering
potential
therapeutic
strategies
ASD.
