Nano-optogenetics for Disease Therapies DOI
Qi Liang Lu,

Yaru Sun,

Zhengbing Liang

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(22), P. 14123 - 14144

Published: May 20, 2024

Optogenetic, known as the method of 21 centuries, combines optic and genetic engineering to precisely control photosensitive proteins for manipulation a broad range cellular functions, such flux ions, protein oligomerization dissociation, intercommunication, so on. In this technique, light is conventionally delivered targeted cells through optical fibers or micro light-emitting diodes, always suffering from high invasiveness, wide-field illumination facula, strong absorption, scattering by nontargeted endogenous substance. Light-transducing nanomaterials with advantages spatiotemporal resolution, abundant wireless-excitation manners, easy functionalization recognition specific cells, recently have been widely explored in field optogenetics; however, there remain few challenges restrain its clinical applications. This review summarized recent progress on light-responsive genetically encoded myriad activation strategies use light-transducing their disease-treatment applications, which expected sparking helpful thought push forward preclinical translational uses.

Language: Английский

Nociceptor neurons affect cancer immunosurveillance DOI Creative Commons
Mohammad Balood, Maryam Ahmadi, Tuany Eichwald

et al.

Nature, Journal Year: 2022, Volume and Issue: 611(7935), P. 405 - 412

Published: Nov. 2, 2022

Abstract Solid tumours are innervated by nerve fibres that arise from the autonomic and sensory peripheral nervous systems 1–5 . Whether neo-innervation of pain-initiating neurons affects cancer immunosurveillance remains unclear. Here we show melanoma cells interact with nociceptor neurons, leading to increases in their neurite outgrowth, responsiveness noxious ligands neuropeptide release. Calcitonin gene-related peptide (CGRP)—one such nociceptor-produced neuropeptide—directly exhaustion cytotoxic CD8 + T cells, which limits capacity eliminate melanoma. Genetic ablation TRPV1 lineage, local pharmacological silencing nociceptors antagonism CGRP receptor RAMP1 all reduced tumour-infiltrating leukocytes decreased growth tumours, nearly tripling survival rate mice were inoculated B16F10 cells. Conversely, cell was rescued sensory-neuron-depleted treated recombinant CGRP. As compared wild-type Ramp1 −/ − protected against when co-transplanted into tumour-bearing Rag1 -deficient mice. Single-cell RNA sequencing biopsies patients revealed intratumoral -expressing more exhausted than -negative counterparts, whereas overexpression correlated a poorer clinical prognosis. Overall, our results suggest reducing release tumour-innervating could be strategy improve anti-tumour immunity eliminating immunomodulatory effects on

Language: Английский

Citations

168
Wireless closed-loop optogenetics across the entire dorsoventral spinal cord in mice DOI
Claudia Kathe, Frédéric Michoud, Philipp Schönle

et al.

Nature Biotechnology, Journal Year: 2021, Volume and Issue: 40(2), P. 198 - 208

Published: Sept. 27, 2021

Language: Английский

Citations

90
FcεR1-expressing nociceptors trigger allergic airway inflammation DOI

Théo Crosson,

Jo-Chiao Wang,

Benjamin Doyle

et al.

Journal of Allergy and Clinical Immunology, Journal Year: 2021, Volume and Issue: 147(6), P. 2330 - 2342

Published: Jan. 13, 2021

Language: Английский

Citations

68
Colocalized, bidirectional optogenetic modulations in freely behaving mice with a wireless dual-color optoelectronic probe DOI Creative Commons
Lizhu Li,

Lihui Lu,

Yuqi Ren

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Feb. 11, 2022

Abstract Optogenetic methods provide efficient cell-specific modulations, and the ability of simultaneous neural activation inhibition in same brain region freely moving animals is highly desirable. Here we report bidirectional neuronal activity manipulation accomplished by a wireless, dual-color optogenetic probe synergy with co-expression two spectrally distinct opsins (ChrimsonR stGtACR2) rodent model. The flexible comprises vertically assembled, thin-film microscale light-emitting diodes lateral dimension 125 × 180 µm 2 , showing colocalized red blue emissions enabling chronic vivo operations desirable biocompatibilities. Red or irradiations deterministically evoke silence neurons co-expressing opsins. interferes dopaminergic ventral tegmental area mice, increasing decreasing dopamine levels. Such regulations further generate rewarding aversive behaviors interrogate social interactions among multiple mice. These technologies create numerous opportunities implications for research.

Language: Английский

Citations

64
Bioelectronic medicine: Preclinical insights and clinical advances DOI Creative Commons
Valentin A. Pavlov, Kevin J. Tracey

Neuron, Journal Year: 2022, Volume and Issue: 110(21), P. 3627 - 3644

Published: Sept. 28, 2022

Language: Английский

Citations

56
The Roles of Optogenetics and Technology in Neurobiology: A Review DOI Creative Commons
Wenqing Chen, Chen Li,

Wanmin Liang

et al.

Frontiers in Aging Neuroscience, Journal Year: 2022, Volume and Issue: 14

Published: April 19, 2022

Optogenetic is a technique that combines optics and genetics to control specific neurons. This usually uses adenoviruses encode photosensitive protein. The adenovirus may concentrate in neural region. By shining light on the target nerve region, protein encoded by controlled. Photosensitive proteins controlled can selectively allow ions inside outside cell membrane pass through, resulting inhibition or activation effects. Due high precision minimally invasive, optogenetics has achieved good results many fields, especially field of neuron functions circuits. Significant advances have also been made study clinical diseases. review focuses research neurobiology. These include how use cells, circuits, treat diseases changing state We hoped this will give comprehensive understanding progress

Language: Английский

Citations

54
Fatigue-resistant hydrogel optical fibers enable peripheral nerve optogenetics during locomotion DOI
Xinyue Liu, Siyuan Rao, Weixuan Chen

et al.

Nature Methods, Journal Year: 2023, Volume and Issue: 20(11), P. 1802 - 1809

Published: Oct. 19, 2023

Language: Английский

Citations

37
Body-conformable light-emitting materials and devices DOI
Zhitao Zhang, Yue Wang, Sisi Jia

et al.

Nature Photonics, Journal Year: 2023, Volume and Issue: 18(2), P. 114 - 126

Published: Dec. 27, 2023

Language: Английский

Citations

31
Compliant peripheral nerve interfaces DOI Creative Commons
Valentina Paggi, Outman Akouissi, Silvestro Micera

et al.

Journal of Neural Engineering, Journal Year: 2021, Volume and Issue: 18(3), P. 031001 - 031001

Published: March 8, 2021

Peripheral nerve interfaces (PNIs) record and/or modulate neural activity of nerves, which are responsible for conducting sensory-motor information to and from the central nervous system, regulating inner organs. PNIs used both in neuroscience research therapeutical applications such as precise closed-loop control neuroprosthetic limbs, treatment neuropathic pain restoration vital functions (e.g. breathing bladder management). Implantable represent an attractive solution directly access peripheral nerves provide enhanced selectivity recording stimulation, compared their non-invasive counterparts. Nevertheless, long-term functionality implantable is limited by tissue damage, occurs at implant-tissue interface, thus highly dependent on material properties, biocompatibility implant design. Current focuses development mechanically compliant PNIs, adapt anatomy dynamic movements body thereby limiting foreign response. In this paper, we review recent progress flexible highlighting promising solutions related materials selection associated fabrication methods, integrated functions. We report variety available interface designs (intraneural, extraneural regenerative) different modulation techniques (electrical, optical, chemical) emphasizing main challenges with integrating systems substrates.

Language: Английский

Citations

48
GPR120 modulates epileptic seizure and neuroinflammation mediated by NLRP3 inflammasome DOI Creative Commons

Zhangjin Qin,

Jiaqi Song, Aolei Lin

et al.

Journal of Neuroinflammation, Journal Year: 2022, Volume and Issue: 19(1)

Published: May 27, 2022

The complex pathophysiology of epilepsy hampers the development effective treatments. Although more than ten kinds anti-seizures drugs (ASDs) have good effects on seizure control worldwide, about 30% patients still display pharmacoresistance against ASDs. Neuroinflammation seems to play a crucial role in disease progression. G protein-coupled receptor 120 (GPR120) has been shown negatively regulate inflammation and apoptosis. However, GPR120 remains unclear. In this study, we aimed explore mechanism epilepsy.Male adult C57BL/6 mice were intracranially injected with kainic acid (KA) establish model, adeno associated virus (AAV) was administered at 3 weeks before KA injection. VX765 by intragastric administration 30 min induced an equal dose administrated twice day (10 a.m. 4 p.m.) lasting 7 days until killed. Western blot analysis, immunofluorescence staining, video monitoring seizure, LFP recording, Nissl staining performed.GPR120 increased both hippocampus cortex KA-induced model temporal lobe (TLE), most highly expressed after Overexpression significantly alleviated epileptic activity, reduced neuronal death status epilepticus (SE), downregulated expression IL-1β, IL-6, IL-18, pyrin domain-containing protein (NLRP3) inflammasome, whereas knockdown showed opposite effect. reversed inhibition cysteinyl aspartate specific proteinase-1 (Caspase-1).GPR120 modulates activity affects survival mouse epilepsy. Furthermore, regulated neuroinflammation animals through NLRP3/Caspase-1/IL-1β signaling pathway.

Language: Английский

Citations

38