Fluorescence Microscopy in Adeno-Associated Virus Research DOI Creative Commons

Susanne K. Golm,

Wolfgang Hübner, Kristian M. Müller

et al.

Viruses, Journal Year: 2023, Volume and Issue: 15(5), P. 1174 - 1174

Published: May 16, 2023

Research on adeno-associated virus (AAV) and its recombinant vectors as well fluorescence microscopy imaging is rapidly progressing driven by clinical applications new technologies, respectively. The topics converge, since high super-resolution microscopes facilitate the study of spatial temporal aspects cellular biology. Labeling methods also evolve diversify. We review these interdisciplinary developments provide information technologies used biological knowledge gained. emphasis lies visualization AAV proteins chemical fluorophores, protein fusions antibodies for detection viral DNA. add a short overview fluorescent microscope techniques their advantages challenges in detecting AAV.

Language: Английский

Spatially exploring RNA biology in archival formalin-fixed paraffin-embedded tissues DOI Creative Commons
Zhiliang Bai, Dingyao Zhang, Yan Gao

et al.

Cell, Journal Year: 2024, Volume and Issue: 187(23), P. 6760 - 6779.e24

Published: Sept. 30, 2024

The capability to spatially explore RNA biology in formalin-fixed paraffin-embedded (FFPE) tissues holds transformative potential for histopathology research. Here, we present pathology-compatible deterministic barcoding tissue (Patho-DBiT) by combining situ polyadenylation and computational innovation spatial whole transcriptome sequencing, tailored probe the diverse species clinically archived FFPE samples. It permits co-profiling of gene expression processing, unveiling region-specific splicing isoforms, high-sensitivity transcriptomic mapping clinical tumor stored 5 years. Furthermore, genome-wide single-nucleotide variants can be captured distinguish malignant subclones from non-malignant cells human lymphomas. Patho-DBiT also maps microRNA regulatory networks dynamics, decoding their roles tumorigenesis. Single-cell level dissects spatiotemporal cellular dynamics driving clonal architecture progression. stands poised as a valuable platform unravel rich aid pathology evaluation.

Language: Английский

Citations

25
Spatial Transcriptomics: Technical Aspects of Recent Developments and Their Applications in Neuroscience and Cancer Research DOI Creative Commons
Han‐Eol Park,

Song Hyun Jo,

Rosalind H. Lee

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(16)

Published: April 7, 2023

Spatial transcriptomics is a newly emerging field that enables high-throughput investigation of the spatial localization transcripts and related analyses in various applications for biological systems. By transitioning from conventional studies to "in situ" biology, can provide transcriptome-scale information. Currently, ability simultaneously characterize gene expression profiles cells relevant cellular environment paradigm shift studies. In this review, recent progress its neuroscience cancer are highlighted. Technical aspects existing technologies future directions new developments (as March 2023), computational analysis transcriptome data, application notes studies, discussions regarding multi-omics their expanding roles biomedical emphasized.

Language: Английский

Citations

40
Advances in AAV technology for delivering genetically encoded cargo to the nonhuman primate nervous system DOI Creative Commons
Lillian Campos, Cynthia M. Arokiaraj, Miguel R. Chuapoco

et al.

Current Research in Neurobiology, Journal Year: 2023, Volume and Issue: 4, P. 100086 - 100086

Published: Jan. 1, 2023

Modern neuroscience approaches including optogenetics, calcium imaging, and other genetic manipulations have facilitated our ability to dissect specific circuits in rodent models study their role neurological disease. These regularly use viral vectors deliver cargo (e.g., opsins) tissues genetically-engineered rodents achieve cell-type specificity. However, the translatability of these models, cross-species validation identified targets, translational efficacy potential therapeutics larger animal like nonhuman primates remains difficult due lack efficient primate vectors. A refined understanding nervous system promises insights that can guide development treatments for neurodegenerative diseases. Here, we outline recent advances adeno-associated optimized primates. tools promise help open new avenues further brain.

Language: Английский

Citations

25
Nucleic acid-based drugs for patients with solid tumours DOI
Sebastian G. Huayamares, David Loughrey, Hyejin Kim

et al.

Nature Reviews Clinical Oncology, Journal Year: 2024, Volume and Issue: 21(6), P. 407 - 427

Published: April 8, 2024

Language: Английский

Citations

16
Natural Adeno-Associated Virus Serotypes and Engineered Adeno-Associated Virus Capsid Variants: Tropism Differences and Mechanistic Insights DOI Creative Commons
Estrella López-Gordo, Kyle Chamberlain, Jalish M. Riyad

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(3), P. 442 - 442

Published: March 12, 2024

Today, adeno-associated virus (AAV)-based vectors are arguably the most promising in vivo gene delivery vehicles for durable therapeutic expression. Advances molecular engineering, high-throughput screening platforms, and computational techniques have resulted a toolbox of capsid variants with enhanced performance over parental serotypes. Despite their considerable promise emerging clinical success, there still obstacles hindering broader use, including limited transduction capabilities, tissue/cell type-specific tropism penetration into tissues through anatomical barriers, off-target tissue biodistribution, intracellular degradation, immune recognition, lack translatability from preclinical models to settings. Here, we first describe mechanisms natural AAV serotypes explore current understanding systemic cellular hurdles efficient transduction. We then outline progress developing designer variants, highlighting seminal discoveries which can transduce central nervous system upon administration, and, lesser extent, discuss targeting peripheral system, eye, ear, lung, liver, heart, skeletal muscle, emphasizing cell specificity translational promise. In particular, dive deeper behind properties, focus on engagement host receptors previously inaccessible Finally, summarize main findings our review future directions.

Language: Английский

Citations

15
An enhancer-AAV toolbox to target and manipulate distinct interneuron subtypes DOI Creative Commons
Elisabetta Furlanis, Min Dai,

Brenda Leyva Garcia

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 22, 2024

SUMMARY In recent years, we and others have identified a number of enhancers that, when incorporated into rAAV vectors, can restrict the transgene expression to particular neuronal populations. Yet, viral tools access manipulate fine subtypes are still limited. Here, performed systematic analysis single cell genomic data identify enhancer candidates for each cortical interneuron subtypes. We established set enhancer-AAV that highly specific distinct populations striatal cholinergic neurons. These enhancers, used in context different effectors, target (fluorescent proteins), observe activity (GCaMP) (opto- or chemo-genetics) also validated our across species. Thus, provide field with powerful study neural circuits functions develop precise targeted therapy.

Language: Английский

Citations

15
Spatially Exploring RNA Biology in Archival Formalin-Fixed Paraffin-Embedded Tissues DOI Creative Commons
Zhiliang Bai, Dingyao Zhang, Yan Gao

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 8, 2024

Spatial transcriptomics has emerged as a powerful tool for dissecting spatial cellular heterogeneity but of today is largely limited to gene expression analysis. Yet, the life RNA molecules multifaceted and dynamic, requiring profiling different species throughout cycle delve into intricate biology in complex tissues. Human disease-relevant tissues are commonly preserved formalin-fixed paraffin-embedded (FFPE) blocks, representing an important resource human tissue specimens. The capability spatially explore FFPE holds transformative potential research clinical histopathology. Here, we present Patho-DBiT combining situ polyadenylation deterministic barcoding full coverage transcriptome sequencing, tailored probing diverse landscape even clinically archived samples. It permits co-profiling processing, unveiling region-specific splicing isoforms, high-sensitivity transcriptomic mapping tumor stored five years. Furthermore, genome-wide single nucleotide variants can be captured distinguish malignant clones from non-malignant cells lymphomas. also maps microRNA-mRNA regulatory networks dynamics, decoding their roles tumorigenesis trajectory. High resolution at level reveals neighborhood traces spatiotemporal kinetics driving progression. stands poised valuable platform unravel rich study aid pathology evaluation.

Language: Английский

Citations

9
Exploring the potential of cell-derived vesicles for transient delivery of gene editing payloads DOI
Kevin Leandro, David Rufino-Ramos, Koen Breyne

et al.

Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 211, P. 115346 - 115346

Published: June 6, 2024

Language: Английский

Citations

8
Spatial omics advances for in situ RNA biology DOI
Jingyi Ren, Shuchen Luo, Hailing Shi

et al.

Molecular Cell, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

Citations

6
Bibliometric and visual analysis of single-cell sequencing from 2010 to 2022 DOI Creative Commons
Ling Chen, Yantong Wan, Tingting Yang

et al.

Frontiers in Genetics, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 11, 2024

Single-cell sequencing (SCS) is a technique used to analyze the genome, transcriptome, epigenome, and other genetic data at level of single cell. The procedure commonly utilized in multiple fields, including neurobiology, immunology, microbiology, has emerged as key focus life science research. However, thorough impartial analysis existing state trends SCS-related research lacking. current study aimed map development studies on SCS during years 2010-2022 through bibliometric software.

Language: Английский

Citations

5