Biofoundries: Bridging Automation and Biomanufacturing in Synthetic Biology

Biotechnology and Bioprocess Engineering, Journal Year: 2023, Volume and Issue: 28(6), P. 892 - 904

Published: Dec. 1, 2023

Language: Английский

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Extracellular vesicles as a potential delivery platform for CRISPR-Cas based therapy in epithelial ovarian cancer

Seminars in Cancer Biology, Journal Year: 2023, Volume and Issue: 96, P. 64 - 81

Published: Oct. 10, 2023

Language: Английский

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Combinatorial metabolic engineering of Bacillus subtilis enables the efficient biosynthesis of isoquercitrin from quercetin

Microbial Cell Factories, Journal Year: 2024, Volume and Issue: 23(1)

Published: April 20, 2024

Isoquercitrin (quercetin-3-O-β-D-glucopyranoside) has exhibited promising therapeutic potentials as cardioprotective, anti-diabetic, anti-cancer, and anti-viral agents. However, its structural complexity limited natural abundance make both bulk chemical synthesis extraction from medical plants difficult. Microbial biotransformation through heterologous expression of glycosyltransferases offers a safe sustainable route for production. Despite several attempts reported in microbial hosts, the current production levels isoquercitrin still lag behind industrial standards.

Language: Английский

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Genome‐Wide CRISPRi Screening of Key Genes for Recombinant Protein Expression in Bacillus Subtilis

Advanced Science, Journal Year: 2024, Volume and Issue: 11(33)

Published: July 1, 2024

Bacillus subtilis is an industrially important microorganism that often used as a microbial cell factory for the production of recombinant proteins due to its food safety, rapid growth, and powerful secretory capacity. However, lack data on functional genes related protein has hindered further development B. factories. Here, strategy combining genome-wide CRISPRi screening targeted CRISPRa activation enhance expression proposed. First, library covering total 4225 coding (99.7%) in genome built corresponding high-throughput methods constructed. Twelve key are identified, including targets without relevant annotations. Meanwhile, transcription by up-regulated. These screened or selected can be easily applied metabolic engineering constructing sgRNA arrays. The relationship between differential pathways engineered strains transcriptome analysis also revealed. High-density fermentation generalisability validation results prove reliability strategy. This method extended other industrial hosts support gene annotation design novel

Language: Английский

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Engineered autonomous dynamic regulation of metabolic flux

Nature Reviews Bioengineering, Journal Year: 2023, Volume and Issue: 2(3), P. 233 - 243

Published: Dec. 21, 2023

Language: Английский

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