Advanced Science,
Journal Year:
2021,
Volume and Issue:
8(20)
Published: Aug. 19, 2021
The
efficacy
of
cardiac
regenerative
strategies
for
myocardial
infarction
(MI)
treatment
is
greatly
limited
by
the
microenvironment.
combination
reactive
oxygen
species
(ROS)
scavenging
to
suppress
oxidative
stress
damage
and
macrophage
polarization
M2
phenotype
in
MI
microenvironment
can
be
desirable
treatment.
Herein,
melanin
nanoparticles
(MNPs)/alginate
(Alg)
hydrogels
composed
two
marine-derived
natural
biomaterials,
MNPs
obtained
from
cuttlefish
ink
alginate
extracted
ocean
algae,
are
proposed.
Taking
advantage
antioxidant
property
mechanical
support
injectable
hydrogels,
MNPs/Alg
hydrogel
explored
repair
regulating
found
eliminate
ROS
against
injury
cardiomyocytes.
More
interestingly,
macrophages
promoted
presence
hydrogel.
An
rat
model
utilized
evaluate
feasibility
as-prepared
vivo.
antioxidant,
anti-inflammatory,
proangiogenesis
effects
investigated
detail.
present
study
opens
up
a
new
way
utilize
biomaterials
allows
rerecognize
great
value
repair.
Nature,
Journal Year:
2020,
Volume and Issue:
588(7838), P. 466 - 472
Published: Sept. 24, 2020
Abstract
Cardiovascular
disease
is
the
leading
cause
of
death
worldwide.
Advanced
insights
into
mechanisms
and
therapeutic
strategies
require
a
deeper
understanding
molecular
processes
involved
in
healthy
heart.
Knowledge
full
repertoire
cardiac
cells
their
gene
expression
profiles
fundamental
first
step
this
endeavour.
Here,
using
state-of-the-art
analyses
large-scale
single-cell
single-nucleus
transcriptomes,
we
characterize
six
anatomical
adult
heart
regions.
Our
results
highlight
cellular
heterogeneity
cardiomyocytes,
pericytes
fibroblasts,
reveal
distinct
atrial
ventricular
subsets
with
diverse
developmental
origins
specialized
properties.
We
define
complexity
vasculature
its
changes
along
arterio-venous
axis.
In
immune
compartment,
identify
cardiac-resident
macrophages
inflammatory
protective
transcriptional
signatures.
Furthermore,
cell-to-cell
interactions
different
networks
macrophages,
fibroblasts
cardiomyocytes
between
atria
ventricles
that
are
from
those
skeletal
muscle.
human
cell
atlas
improves
our
provides
valuable
reference
for
future
studies.
Science,
Journal Year:
2019,
Volume and Issue:
363(6432)
Published: March 14, 2019
Tissue
macrophages
have
a
split
personality
Resident
tissue
(RTMs)
reside
in
various
tissue-specific
niches
during
development.
They
evince
microenvironment-directed
phenotypes
that
support
host
defense
and
homeostasis.
Chakarov
et
al.
used
single-cell
RNA
sequencing
fate-mapping
of
murine
lung
RTMs
to
interrogate
RTM-subset
heterogeneity,
interrelationships,
ontogeny
(see
the
Perspective
by
Mildner
Yona).
In
addition
alveolar
macrophages,
they
identified
two
different
interstitial
macrophage
populations.
One
population
mostly
abutted
nerve
fibers;
other
preferentially
localized
near
blood
vessels
appeared
vessel
integrity
inhibit
inflammatory
cell
infiltration
into
tissues.
Science
,
this
issue
p.
eaau0964
;
see
also
1154
Journal of Clinical Investigation,
Journal Year:
2019,
Volume and Issue:
129(7), P. 2619 - 2628
Published: May 19, 2019
Macrophages
are
tissue-resident
or
infiltrated
immune
cells
critical
for
innate
immunity,
normal
tissue
development,
homeostasis,
and
repair
of
damaged
tissue.
Macrophage
function
is
a
sum
their
ontogeny,
the
local
environment
in
which
they
reside,
type
injuries
pathogen
to
exposed.
In
this
Review,
we
discuss
role
macrophages
restoration
after
injury,
highlighting
important
questions
about
how
respond
modify
microenvironment
restore
homeostasis.
Besides
cardiomyocytes
(CM),
the
heart
contains
numerous
interstitial
cell
types
which
play
key
roles
in
repair,
regeneration
and
disease,
including
fibroblast,
vascular
immune
cells.
However,
a
comprehensive
understanding
of
this
interactive
community
is
lacking.
We
performed
single-cell
RNA-sequencing
total
non-CM
fraction
enriched
(Pdgfra-GFP+)
fibroblast
lineage
cells
from
murine
hearts
at
days
3
7
post-sham
or
myocardial
infarction
(MI)
surgery.
Clustering
>30,000
single
identified
>30
populations
representing
nine
lineages,
previously
undescribed
trajectory
present
both
sham
MI
leading
to
uniquely
activated
state
defined
part
by
strong
anti-WNT
transcriptome
signature.
also
uncovered
novel
myofibroblast
subtypes
expressing
either
pro-fibrotic
anti-fibrotic
signatures.
Our
data
highlight
non-linear
dynamics
myeloid
lineages
after
cardiac
injury,
provide
an
entry
point
for
deeper
analysis
homeostasis,
inflammation,
fibrosis,
repair
regeneration.
Circulation Research,
Journal Year:
2020,
Volume and Issue:
126(6), P. 789 - 806
Published: March 12, 2020
Obesity
and
hypertension,
which
often
coexist,
are
major
risk
factors
for
heart
failure
characterized
by
chronic,
low-grade
inflammation,
promotes
adverse
cardiac
remodeling.
While
macrophages
play
a
key
role
in
remodeling,
dysregulation
of
macrophage
polarization
between
the
proinflammatory
M1
anti-inflammatory
M2
phenotypes
excessive
inflammation
injury.
Metabolic
shifting
glycolysis
mitochondrial
oxidative
phosphorylation
has
been
implicated
polarization.
primarily
rely
on
glycolysis,
whereas
tricarboxylic
acid
cycle
phosphorylation;
thus,
that
affect
metabolism
may
disrupt
M1/M2
homeostasis
exacerbate
inflammation.
The
mechanisms
obesity
hypertension
synergistically
induce
metabolic
dysfunction,
particularly
during
not
fully
understood.
We
propose
via
directly
target
metabolism,
including
changes
circulating
glucose
fatty
substrates,
lipotoxicity,
tissue
hypoxia.
discuss
canonical
novel
roles
obesity-hypertension-induced
injury,
diastolic
dysfunction
impaired
calcium
handling.
Finally,
we
current
status
potential
therapies
to
failure,
antidiabetic
therapies,
immunometabolic
agents.
