Cell Reports Methods,
Journal Year:
2022,
Volume and Issue:
2(4), P. 100192 - 100192
Published: March 28, 2022
Macrophages
are
dynamic
immune
cells
that
can
adopt
several
activation
states.
Fundamental
to
these
functional
states
is
the
regulation
of
cellular
metabolic
processes.
Especially
in
mice,
alterations
underlying
pro-inflammatory
or
homeostatic
phenotypes
have
been
assessed
using
various
techniques.
However,
researchers
new
field
may
encounter
ambiguity
choosing
which
combination
techniques
best
suited
profile
immunometabolism.
To
address
this
need,
we
developed
a
toolbox
assess
metabolism
semi-high-throughput
96-well-plate-based
format.
Application
activated
mouse
and
human
macrophages
enables
fast
pre-screening
robust
measurement
extracellular
fluxes,
mitochondrial
mass
membrane
potential,
glucose
lipid
uptake.
Moreover,
propose
an
application
SCENITH
technology
for
Signal Transduction and Targeted Therapy,
Journal Year:
2023,
Volume and Issue:
8(1)
Published: Nov. 22, 2023
The
intricacy
of
diseases,
shaped
by
intrinsic
processes
like
immune
system
exhaustion
and
hyperactivation,
highlights
the
potential
renormalization
as
a
promising
strategy
in
disease
treatment.
In
recent
years,
our
primary
focus
has
centered
on
γδ
T
cell-based
immunotherapy,
particularly
pioneering
use
allogeneic
Vδ2
Signal Transduction and Targeted Therapy,
Journal Year:
2023,
Volume and Issue:
8(1)
Published: March 22, 2023
Abstract
Tumour
cells
have
exquisite
flexibility
in
reprogramming
their
metabolism
order
to
support
tumour
initiation,
progression,
metastasis
and
resistance
therapies.
These
reprogrammed
activities
include
a
complete
rewiring
of
the
bioenergetic,
biosynthetic
redox
status
sustain
increased
energetic
demand
cells.
Over
last
decades,
cancer
field
has
seen
an
explosion
new
biochemical
technologies
giving
more
tools
than
ever
before
navigate
this
complexity.
Within
cell
or
tissue,
metabolites
constitute
direct
signature
molecular
phenotype
thus
profiling
concrete
clinical
applications
oncology.
Metabolomics
fluxomics,
are
key
technological
approaches
that
mainly
revolutionized
enabling
researchers
both
qualitative
mechanistic
model
cancer.
Furthermore,
upgrade
from
bulk
single-cell
analysis
provided
unprecedented
opportunity
investigate
biology
at
cellular
resolution
allowing
depth
quantitative
complex
heterogenous
diseases.
More
recently,
advent
functional
genomic
screening
allowed
identification
pathways,
processes,
biomarkers
novel
therapeutic
targets
concert
with
other
allow
patient
stratification
treatment
regimens.
This
review
is
intended
be
guide
for
metabolism,
highlighting
current
emerging
technologies,
emphasizing
advantages,
disadvantages
potential
leading
development
innovative
anti-cancer
Science,
Journal Year:
2022,
Volume and Issue:
377(6603), P. 276 - 284
Published: July 14, 2022
γδ
T
cells
represent
a
substantial
fraction
of
intestinal
lymphocytes
at
homeostasis,
but
they
also
constitute
major
lymphocyte
population
infiltrating
colorectal
cancers
(CRCs);
however,
their
temporal
contribution
to
CRC
development
or
progression
remains
unclear.
Using
human
samples
and
murine
models,
we
found
that
most
in
premalignant
nontumor
colons
exhibit
cytotoxic
markers,
whereas
tumor-infiltrating
express
protumorigenic
profile.
These
contrasting
cell
profiles
were
associated
with
distinct
receptor
(TCR)-Vγδ
gene
usage
both
humans
mice.
Longitudinal
intersectional
genetics
antibody-dependent
strategies
targeting
enriched
the
epithelium
steady
state
led
heightened
tumor
development,
subsets
accumulate
during
resulted
reduced
growth.
Our
results
uncover
pro-
antitumor
roles
for
subsets.
Cellular and Molecular Immunology,
Journal Year:
2022,
Volume and Issue:
19(3), P. 432 - 444
Published: Jan. 5, 2022
Tumour
growth
and
dissemination
is
largely
dependent
on
nutrient
availability.
It
has
recently
emerged
that
the
tumour
microenvironment
rich
in
a
diverse
array
of
lipids
increase
abundance
with
progression
play
role
promoting
metastasis.
Here,
we
describe
pro-tumorigenic
roles
lipid
uptake,
metabolism
synthesis
detail
therapeutic
potential
targeting
cancer.
Additionally,
highlight
new
insights
into
distinct
immunosuppressive
effects
microenvironment.
Lipids
threaten
an
anti-tumour
environment
whereby
metabolic
adaptation
to
linked
immune
dysfunction.
Finally,
differential
commondietary
cancer
which
may
uncover
for
specific
dietary
regimens
association
traditional
therapies.
Understanding
relationship
between
lipids,
tumour,
cells
important
context
obesity
reveal
possibility
harness
diet
treatment
cancers.
Molecular Cancer,
Journal Year:
2023,
Volume and Issue:
22(1)
Published: Feb. 15, 2023
Abstract
As
a
nontraditional
T-cell
subgroup,
γδT
cells
have
gained
popularity
in
the
field
of
immunotherapy
recent
years.
They
extraordinary
antitumor
potential
and
prospects
for
clinical
application.
Immune
checkpoint
inhibitors
(ICIs),
which
are
efficacious
tumor
patients,
become
pioneer
drugs
since
they
were
incorporated
into
practice.
In
addition,
that
infiltrated
tissues
found
to
be
state
exhaustion
or
anergy,
there
is
upregulation
many
immune
checkpoints
(ICs)
on
their
surface,
suggesting
similar
ability
respond
ICIs
as
traditional
effector
T
cells.
Studies
shown
targeting
ICs
can
reverse
dysfunctional
microenvironment
(TME)
exert
effects
by
improving
γδT-cell
proliferation
activation
enhancing
cytotoxicity.
Clarification
functional
TME
mechanisms
underlying
interaction
with
will
solidify
combined
good
treatment
option.
Cell Death and Disease,
Journal Year:
2024,
Volume and Issue:
15(2)
Published: Feb. 1, 2024
Abstract
Although
immunotherapy
has
made
breakthrough
progress,
its
efficacy
in
solid
tumours
remains
unsatisfactory.
Exosomes
are
the
main
type
of
extracellular
vesicles
that
can
deliver
various
intracellular
molecules
to
adjacent
or
distant
cells
and
organs,
mediating
biological
functions.
Studies
have
found
exosomes
both
activate
immune
system
inhibit
system.
The
antigen
major
histocompatibility
complex
(MHC)
carried
make
it
possible
develop
them
as
anticancer
vaccines.
derived
from
blood,
urine,
saliva
cerebrospinal
fluid
be
used
ideal
biomarkers
cancer
diagnosis
prognosis.
In
recent
years,
exosome-based
therapy
great
progress
fields
drug
transportation
immunotherapy.
Here,
we
review
composition
sources
microenvironment
further
elaborate
on
potential
mechanisms
pathways
by
which
influence
for
cancers.
Moreover,
summarize
clinical
application
prospects
engineered
exosome
vaccines
Eventually,
these
findings
may
open
up
avenues
determining
diagnosis,
treatment,
prognosis
Journal of Clinical Investigation,
Journal Year:
2024,
Volume and Issue:
134(7)
Published: Jan. 30, 2024
The
measles,
mumps
and
rubella
(MMR)
vaccine
protects
against
all-cause
mortality
in
children,
but
the
immunological
mechanisms
mediating
these
effects
are
poorly
known.
We
systematically
investigated
whether
MMR
can
induce
long-term
functional
changes
innate
immune
cells,
a
process
termed
trained
immunity,
that
could
at
least
partially
mediate
this
heterologous
protection.
In
randomized
placebo-controlled
trial,
39
healthy
adults
received
either
or
placebo.
By
using
single-cell
RNA-sequencing,
we
found
caused
transcriptomic
CD14-positive
monocytes
NK
most
profoundly
γδ
T
cells.
Monocyte
function
was
not
altered
by
vaccination.
contrast,
of
cells
markedly
enhanced
vaccination,
with
higher
production
TNF
IFNγ,
as
well
upregulation
cellular
metabolic
pathways.
conclusion,
describe
immunity
program
characterized
modulation
cell
induced
