Deep learning pipeline reveals key moments in human embryonic development predictive of live birth in IVF DOI Creative Commons
Camilla Mapstone, Helen Hunter, Daniel R. Brison

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 28, 2023

Abstract Demand for IVF treatment is growing, however success rates remain low partly due to difficulty in selecting the best embryo be transferred. Current manual assessments are subjective and may not take advantage of most informative moments development. Here, we apply convolutional neural networks (CNNs) identify key windows preimplantation human development that can linked viability therefore suitable early grading embryos. We show how machine learning models trained at these developmental time-points used refine overall assessment. Exploiting well-known capabilities transfer learning, illustrate performance CNN very limited data sets, paving way use on a clinic-by-clinic basis, catering local heterogeneity.

Language: Английский

The Burden of Proof studies: assessing the evidence of risk DOI Creative Commons

Peng Zheng,

Ashkan Afshin,

Stan Biryukov

et al.

Nature Medicine, Journal Year: 2022, Volume and Issue: 28(10), P. 2038 - 2044

Published: Oct. 1, 2022

Abstract Exposure to risks throughout life results in a wide variety of outcomes. Objectively judging the relative impact these on personal and population health is fundamental individual survival societal prosperity. Existing mechanisms quantify rank magnitude myriad effects uncertainty their estimation are largely subjective, leaving room for interpretation that can fuel academic controversy add confusion when communicating risk. We present new suite meta-analyses—termed Burden Proof studies—designed specifically help evaluate methodological issues objectively quantitatively. Through this data-driven approach complements existing systems, including GRADE Cochrane Reviews, we aim aggregate evidence across multiple studies enable quantitative comparison risk–outcome pairs. introduce burden proof risk function (BPRF), which estimates level closest null hypothesis consistent with available data. Here illustrate BPRF methodology evaluation four exemplar pairs: smoking lung cancer, systolic blood pressure ischemic heart disease, vegetable consumption unprocessed red meat disease. The strength each relationship assessed by computing summarizing BPRF, then translating summary simple star rating. provides way understand, summarize different pairs, informs analysis conducted as part Global Diseases, Injuries, Risk Factors Study.

Language: Английский

Citations

72
The mammalian preimplantation embryo: Its role in the environmental programming of postnatal health and performance DOI
Miguel A. Velazquez,

Abdullah Idriss,

Pascale Chavatte‐Palmer

et al.

Animal Reproduction Science, Journal Year: 2023, Volume and Issue: 256, P. 107321 - 107321

Published: Aug. 19, 2023

Language: Английский

Citations

16
Cohort Profile: The Jiangsu Birth Cohort DOI
Jiangbo Du, Yuan Lin, Yankai Xia

et al.

International Journal of Epidemiology, Journal Year: 2023, Volume and Issue: 52(6), P. e354 - e363

Published: Nov. 4, 2023

Journal Article Cohort Profile: The Jiangsu Birth Get access Jiangbo Du, Du State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing, ChinaDepartment Epidemiology Biostatistics, School Public China Search for other works by this author on: Oxford Academic PubMed Google Scholar Yuan Lin, Lin Maternal, Child Adolescent Center Global Yankai Xia, Xia ChinaKey Modern Toxicology Ministry Education, https://orcid.org/0000-0003-0484-4035 Hongxia Ma, Ma https://orcid.org/0000-0002-2462-9693 Yangqian Jiang, Jiang Department Chuncheng Lu, Lu https://orcid.org/0000-0003-3689-9188 Wei Wu, Wu https://orcid.org/0000-0002-0267-1284 Minjian Chen, Chen Yang Zhao, Zhao Juncheng Dai, Dai ... Show more Guangfu Jin, Jin Jiayin Liu, Liu Jiahao Sha, Sha Hongbing Shen, Shen https://orcid.org/0000-0002-2581-5906 Zhibin Hu, Hu Corresponding author. 101 Longmian Avenue, 21116, China. E-mail: [email protected] https://orcid.org/0000-0002-8277-5234 National (CNBC) Study Group International Epidemiology, Volume 52, Issue 6, December 2023, Pages e354–e363, https://doi.org/10.1093/ije/dyad139 Published: 04 November 2023 history Received: 29 2022 Editorial decision: 14 September Accepted: 28

Language: Английский

Citations

11
Telomere length in offspring is determined by mitochondrial-nuclear communication at fertilization DOI Creative Commons
Yasmyn E. Winstanley, Ryan Rose, Alexander P. Sobinoff

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 14, 2025

Abstract The initial setting of telomere length during early life in each individual has a major influence on lifetime risk aging-associated diseases; however there is limited knowledge biological signals that regulate inheritance length, and whether it modifiable not known. We now show when mitochondrial activity disrupted mouse zygotes, via exposure to 20% O 2 or rotenone, elongation between the 8-cell blastocyst stage impaired, with shorter telomeres apparent pluripotent Inner Cell Mass (ICM) persisting after organogenesis. Identical defects elevated mtROS zygotes followed by impaired elongation, occurred maternal obesity advanced age. further demonstrate ICM formation controlled mitochondrial-nuclear communication at fertilization. Using mitochondrially-targeted therapeutics (BGP-15, MitoQ, SS-31, metformin) we possible modulate preimplantation resetting process restore deficiencies neonatal length.

Language: Английский

Citations

0
Microbiome–metabolome dynamics associated with impaired glucose control and responses to lifestyle changes DOI Creative Commons
Hao Wu,

Bo‐Min Lv,

Luqian Zhi

et al.

Nature Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

Abstract Type 2 diabetes (T2D) is a complex disease shaped by genetic and environmental factors, including the gut microbiome. Recent research revealed pathophysiological heterogeneity distinct subgroups in both T2D prediabetes, prompting exploration of personalized risk factors. Using metabolomics two Swedish cohorts ( n = 1,167), we identified over 500 blood metabolites associated with impaired glucose control, approximately one-third linked to an altered Our findings metabolic disruptions microbiome–metabolome dynamics as potential mediators compromised homeostasis, illustrated interactions between Hominifimenecus microfluidus Blautia wexlerae via hippurate. Short-term lifestyle changes, for example, diet exercise, modulated microbiome-associated lifestyle-specific manner. This study suggests that axis modifiable target management, optimal health benefits achievable through combination modifications.

Language: Английский

Citations

0
Role of Stem Cells in Ageing and Age-related Diseases DOI
Jitendra Kumar Chaudhary, Ajay Kumar Danga, A. Leela Kumari

et al.

Mechanisms of Ageing and Development, Journal Year: 2025, Volume and Issue: unknown, P. 112069 - 112069

Published: May 1, 2025

Language: Английский

Citations

0
Rapid and non-invasive diagnostic techniques for embryonic developmental potential: a metabolomic analysis based on Raman spectroscopy to identify the pregnancy outcomes of IVF-ET DOI Creative Commons
Hui Meng, Shan Huang, Feiyang Diao

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: June 23, 2023

The non-invasive and rapid assessment of the developmental potential embryos is great clinical importance in assisted reproductive technology (ART). In this retrospective study, we analyzed metabolomics 107 samples provided by volunteers utilized Raman spectroscopy to detect substance composition discarded culture medium 53 resulting successful pregnancies 54 that did not result pregnancy after implantation. from D3 cleavage-stage was collected transplantation a total 535 (107 × 5) original spectra were obtained. By combining several machine learning methods, predicted embryos, principal component analysis-convolutional neural network (PCA-CNN) model achieved an accuracy rate 71.5%. Furthermore, chemometric algorithm used analyze seven amino acid metabolites medium, data showed significant differences tyrosine, tryptophan, serine between non-pregnancy groups. results suggest spectroscopy, as molecular fingerprint detection technology, shows for application reproduction.

Language: Английский

Citations

9
Potential effects of assisted reproductive technology on telomere length and telomerase activity in human oocytes and early embryos DOI Creative Commons

Betul Tire,

Saffet Öztürk

Journal of Ovarian Research, Journal Year: 2023, Volume and Issue: 16(1)

Published: July 4, 2023

Abstract Telomeres are repetitive DNA sequences at eukaryotic chromosome ends and function in maintaining genome integrity stability. These unique structures undergo shortening due to various factors including biological aging, consecutive replication, oxidative stress, genotoxic agents. Shortened telomeres can be lengthened by the enzyme telomerase alternative lengthening of germ cells, early embryos, stem activated lymphocytes. If reach critical length, it may lead genomic instability, segregation defects, aneuploidy, apoptosis. phenotypes also occur oocytes produced using assisted reproductive technologies (ARTs). Thus, a number studies have examined potential effects ART applications such as ovarian stimulation, culture conditions, cryopreservation procedures on telomeres. Herein, we comprehensively reviewed impacts these telomere length activity ART-derived embryos. Further, discussed use parameters centers biomarker determining oocyte embryo quality.

Language: Английский

Citations

9
Comorbidities confound metabolomics studies of human disease DOI Creative Commons
Madis Jaagura, Jaanika Kronberg, Anu Reigo

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 22, 2024

The co-occurrence of multiple chronic conditions, termed multimorbidity, presents an expanding global health challenge, demanding effective diagnostics and treatment strategies. Chronic ailments such as obesity, diabetes, cardiovascular diseases have been linked to metabolites interacting between the host microbiota. In this study, we investigated impact co-existing conditions on risk estimations for 1375 plasma in 919 individuals from population-based Estonian Biobank cohort using liquid chromatography mass spectrometry (LC-MS) method. We leveraged annually national electronic records (EHRs) data delineate comorbidities incident cases controls 14 common conditions. Among 254 associations observed across 13 primarily identified disease-specific factors (92%, 217/235), with most predictors (93%, 219/235) found be related gut microbiome upon cross-referencing recent literature data. Accounting led a reduction metabolite various conclusion, our study underscores potential utilizing biobank-linked retrospective prospective EHRs profiling diverse multifactorial

Language: Английский

Citations

3
High expression of RTEL1 predicates worse progression in gliomas and promotes tumorigenesis through JNK/ELK1 cascade DOI Creative Commons
Guanjie Wang,

Xiaojuan Ren,

Jianying Li

et al.

BMC Cancer, Journal Year: 2024, Volume and Issue: 24(1)

Published: March 26, 2024

Abstract Gliomas are the most common primary intracranial tumor worldwide. The maintenance of telomeres serves as an important biomarker some subtypes glioma. In order to investigate biological role RTEL1 in Relative telomere length (RTL) and mRNA was explored regression analysis performed further examine relationship RTL expression with clinicopathological characteristics glioma patients. We observed that high is positively correlated tissue, serve a poor prognostic factor TERT wild-type Further vitro studies demonstrate promoted proliferation, formation, migration invasion ability cells. addition, vivo also revealed oncogene study using RNA sequence phospho-specific antibody microarray assays identified JNK/ELK1 signaling up-regulated by cells through ROS. conclusion, our results suggested promotes tumorigenesis cascade indicate may be gliomas.

Language: Английский

Citations

2