Biomolecules,
Journal Year:
2023,
Volume and Issue:
13(3), P. 450 - 450
Published: Feb. 28, 2023
Cells
use
the
actin
cytoskeleton
for
many
of
their
functions,
including
division,
adhesion,
mechanosensing,
endo-
and
phagocytosis,
migration,
invasion.
Actin
bundles
are
main
constituent
actin-rich
structures
involved
in
these
processes.
An
ever-increasing
number
proteins
that
crosslink
into
or
regulate
morphology
is
being
identified
cells.
With
recent
advances
high-resolution
microscopy
imaging
techniques,
complex
process
formation
multiple
forms
physiological
beginning
to
be
better
understood.
Here,
we
review
physiochemical
biological
properties
four
families
highly
conserved
abundant
actin-bundling
proteins,
namely,
α-actinin,
fimbrin/plastin,
fascin,
espin.
We
describe
similarities
differences
between
role
bundles,
properties—both
related
unrelated
bundling
abilities.
also
some
aspects
general
mechanism
formation,
which
known
from
available
information
on
activity
key
partners
this
process.
Nature Reviews Drug Discovery,
Journal Year:
2022,
Volume and Issue:
21(11), P. 841 - 862
Published: Aug. 16, 2022
In
the
past
decade,
membraneless
assemblies
known
as
biomolecular
condensates
have
been
reported
to
play
key
roles
in
many
cellular
functions
by
compartmentalizing
specific
proteins
and
nucleic
acids
subcellular
environments
with
distinct
properties.
Furthermore,
growing
evidence
supports
view
that
often
form
phase
separation,
which
a
single-phase
system
demixes
into
two-phase
consisting
of
condensed
dilute
particular
biomolecules.
Emerging
understanding
condensate
function
normal
aberrant
states,
mechanisms
formation,
is
providing
new
insights
human
disease
revealing
novel
therapeutic
opportunities.
this
Perspective,
we
propose
such
could
enable
previously
unexplored
drug
discovery
approach
based
on
identifying
condensate-modifying
therapeutics
(c-mods),
discuss
strategies,
techniques
challenges
involved.
Chemical Reviews,
Journal Year:
2021,
Volume and Issue:
122(2), P. 1752 - 1829
Published: Sept. 21, 2021
Chemically
modified
biomacromolecules─i.e.,
proteins,
nucleic
acids,
glycans,
and
lipids─have
become
crucial
tools
in
chemical
biology.
They
are
extensively
used
not
only
to
elucidate
cellular
processes
but
also
industrial
applications,
particularly
the
context
of
biopharmaceuticals.
In
order
enable
maximum
scope
for
optimization,
it
is
pivotal
have
a
diverse
array
biomacromolecule
modification
methods
at
one's
disposal.
Chemistry
has
driven
many
significant
advances
this
area,
especially
recently,
numerous
novel
visible-light-induced
photochemical
approaches
emerged.
these
reactions,
light
serves
as
an
external
source
energy,
enabling
access
highly
reactive
intermediates
under
exceedingly
mild
conditions
with
exquisite
spatiotemporal
control.
While
UV-induced
transformations
on
biomacromolecules
date
back
decades,
visible
unmistakable
advantage
being
considerably
more
biocompatible,
spectrum
visible-light-driven
now
available,
chiefly
proteins
acids.
This
review
will
discuss
modifications
native
functional
groups
(FGs),
including
functionalization,
labeling,
cross-linking
techniques
well
utility
oxidative
degradation
mediated
by
photochemically
generated
oxygen
species.
Furthermore,
non-native,
bioorthogonal
FGs
be
addressed,
photoclick
chemistry
DNA-encoded
library
synthesis
that
allow
manipulation
activity
biomacromolecule.
Journal of Proteome Research,
Journal Year:
2023,
Volume and Issue:
22(7), P. 2151 - 2171
Published: June 1, 2023
Mass
spectrometry
is
unmatched
in
its
versatility
for
studying
practically
any
aspect
of
the
proteome.
Because
foundations
mass
spectrometry-based
proteomics
are
complex
and
span
multiple
scientific
fields,
can
be
perceived
as
having
a
high
barrier
to
entry.
This
tutorial
intended
an
accessible
illustrated
guide
technical
details
relatively
simple
quantitative
proteomic
experiment.
An
attempt
made
explain
relevant
concepts
those
with
limited
knowledge
basic
understanding
proteins.
experimental
overview
provided,
from
beginning
sample
preparation
analysis
protein
group
quantities,
explanations
how
data
acquired,
processed,
analyzed.
A
selection
advanced
topics
briefly
surveyed
works
further
reading
cited.
To
conclude,
brief
discussion
future
given,
considering
next-generation
sequencing
technologies
that
may
complement
create
fruitful
proteomics.
Chemical Reviews,
Journal Year:
2022,
Volume and Issue:
122(10), P. 9497 - 9570
Published: March 31, 2022
In-cell
structural
biology
aims
at
extracting
information
about
proteins
or
nucleic
acids
in
their
native,
cellular
environment.
This
emerging
field
holds
great
promise
and
is
already
providing
new
facts
outlooks
of
interest
both
fundamental
applied
levels.
NMR
spectroscopy
has
important
contributions
on
this
stage:
It
brings
a
broad
variety
nuclei
the
atomic
scale,
which
ensures
its
versatility
uniqueness.
Here,
we
detail
methods,
knowledge,
applications
biomedical
engineering
related
to
in-cell
by
NMR.
We
finally
propose
brief
overview
main
other
techniques
(EPR,
smFRET,
cryo-ET,
etc.)
draw
some
advisable
developments
for
In
era
large-scale
screenings
deep
learning,
accurate
qualitative
experimental
evidence
are
as
essential
ever
understand
interior
life
cells.
can
generate
such
it
does
so
scale.
review
meant
deliver
comprehensive
but
accessible
information,
with
advanced
technical
details
reflections
nature
results,
future
field.
Journal of the American Chemical Society,
Journal Year:
2022,
Volume and Issue:
144(14), P. 6154 - 6162
Published: April 1, 2022
Modern
proximity
labeling
techniques
have
enabled
significant
advances
in
understanding
biomolecular
interactions.
However,
current
tools
primarily
utilize
activation
modes
that
are
incompatible
with
complex
biological
environments,
limiting
our
ability
to
interrogate
cell-
and
tissue-level
microenvironments
animal
models.
Here,
we
report
μMap-Red,
a
platform
uses
red-light-excited
Sn
