Microfluidic Technology for the Isolation and Analysis of Exosomes DOI Creative Commons
Yusong Wu, Yuqing Wang, Yanjun Lu

et al.

Micromachines, Journal Year: 2022, Volume and Issue: 13(10), P. 1571 - 1571

Published: Sept. 22, 2022

Exosomes are lipid-bilayer enclosed vesicles with diameters of 30–150 nm, which play a pivotal role in cell communication by transporting their cargoes such as proteins, lipids, and genetic materials. In recent years, exosomes have been under intense investigation, they show great promise numerous areas, especially bio-markers liquid biopsies. However, due to the high heterogeneity nano size exosomes, separation is not easy. This review will deliver an outline conventional methods microfluidic-based technologies for exosome separation. Particular attention devoted microfluidic devices, highlighting efficiency isolation these methods. Additionally, this introduce advances made integrated microfluidics that enable analysis exosomes.

Language: Английский

Exosomes as a new frontier of cancer liquid biopsy DOI Creative Commons
Dan Yu,

Yixin Li,

Maoye Wang

et al.

Molecular Cancer, Journal Year: 2022, Volume and Issue: 21(1)

Published: Feb. 18, 2022

Abstract Liquid biopsy, characterized by minimally invasive detection through biofluids such as blood, saliva, and urine, has emerged a revolutionary strategy for cancer diagnosis prognosis prediction. Exosomes are subset of extracellular vesicles (EVs) that shuttle molecular cargoes from donor cells to recipient play crucial role in mediating intercellular communication. Increasing studies suggest exosomes have great promise serve novel biomarkers liquid since large quantities enriched body fluids involved numerous physiological pathological processes. However, the further clinical application been greatly restrained lack high-quality separation component analysis methods. This review aims provide comprehensive overview on conventional technologies exosome isolation, characterization content detection. Additionally, roles serving potential biopsy diagnosis, treatment monitoring, prediction summarized. Finally, prospects challenges applying exosome-based precision medicine evaluated.

Language: Английский

Citations

557
Small extracellular vesicles isolation and separation: Current techniques, pending questions and clinical applications DOI Creative Commons

Yuanwang Jia,

Yu Li, Tieliang Ma

et al.

Theranostics, Journal Year: 2022, Volume and Issue: 12(15), P. 6548 - 6575

Published: Jan. 1, 2022

Extracellular vesicles, especially small extracellular vesicles (sEVs) are now accepted as important messengers in cell-to-cell communication and a promising drug delivery platform.They involved nearly all physiological pathological processes disease diagnosis therapy.However, their heterogeneity of physicochemical properties functions is not fully understood, which hinders further clinical applications.To obtain highly bioactive sEVs with both high yield purity, will certainly facilitate future study application.This review informs up-to-date research on frequently-used cutting-edge technologies isolation makes deep comparison analysis different methods, including advantages, limitations applications.Pending questions about the inherent property these well strategies discussed.Additionally, an overview applications treatment, some on-going trials, also reviewed.

Language: Английский

Citations

175
High-quality milk exosomes as oral drug delivery system DOI
Jie Zhong, Bozhang Xia,

Shaobo Shan

et al.

Biomaterials, Journal Year: 2021, Volume and Issue: 277, P. 121126 - 121126

Published: Sept. 9, 2021

Language: Английский

Citations

135
Exosomes in the hypoxic TME: from release, uptake and biofunctions to clinical applications DOI Creative Commons
Guangpeng He, Xueqiang Peng, Shibo Wei

et al.

Molecular Cancer, Journal Year: 2022, Volume and Issue: 21(1)

Published: Jan. 17, 2022

Abstract Hypoxia is a remarkable trait of the tumor microenvironment (TME). When facing selective pressure, cells show various adaptive characteristics, such as changes in expression cancer hallmarks (increased proliferation, suppressed apoptosis, immune evasion, and so on) more frequent cell communication. Because adaptation to hypoxia, exploring association between communication mediators hypoxia has become increasingly important. Exosomes are important information carriers cell-to-cell Abundant evidence proven that effects TME mediated by exosomes, with occasional formation feedback loops. In this review, we equally focus on biogenesis heterogeneity cancer-derived exosomes their functions under describe known potential mechanism ascribed hypoxia. Notably, call attention size change hypoxic cell-derived characteristic long neglected, propose some possible change. Finally, jointly considering recent developments understanding tumors, noteworthy problems field urgently need be solved for better research clinical application.

Language: Английский

Citations

129
The Yin and Yang of exosome isolation methods: conventional practice, microfluidics, and commercial kits DOI
Saeedreza Zeibi Shirejini, Fatih İnci

Biotechnology Advances, Journal Year: 2021, Volume and Issue: 54, P. 107814 - 107814

Published: Aug. 10, 2021

Language: Английский

Citations

124
Recent developments in isolating methods for exosomes DOI Creative Commons

Jiahui Gao,

Ang Li, Jie Hu

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 10

Published: Jan. 13, 2023

Exosomes are the smallest extracellular vesicles that can be released by practically all cell types, and range in size from 30 nm to 150 nm. As major marker of liquid biopsies, exosomes have great potential for disease diagnosis, therapy, prognosis. However, their inherent heterogeneity, complexity biological fluids, presence nanoscale contaminants make isolation a challenge. Traditional methods cumbersome challenging with complex time-consuming operations. In recent years, emergence microfluidic chips, nanolithography, electro-deposition, other technologies has promoted combination innovation methods. The application these brought very considerable benefits such as ultra-fast, portable integration, low loss. There significant functional improvements yield, purity, clinical applications. this review, series summarized, emphasis on emerging methods, in-depth comparison analysis each method provided, including principles, merits, demerits.

Language: Английский

Citations

108
Extracellular vesicles and particles impact the systemic landscape of cancer DOI
Serena Lucotti, Candia M. Kenific, Haiying Zhang

et al.

The EMBO Journal, Journal Year: 2022, Volume and Issue: 41(18)

Published: Sept. 2, 2022

Language: Английский

Citations

78
MicroRNAs in extracellular vesicles: Sorting mechanisms, diagnostic value, isolation, and detection technology DOI Creative Commons

Dongjie Xu,

Kaili Di,

Boyue Fan

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10

Published: Oct. 17, 2022

MicroRNAs (miRNAs) are a class of short, single-stranded, noncoding RNAs, with length about 18–22 nucleotides. Extracellular vesicles (EVs) derived from cells and play vital role in the development diseases can be used as biomarkers for liquid biopsy, they carriers miRNA. Existing studies have found that most functions miRNA mainly realized through intercellular transmission EVs, which protect sort miRNAs. Meanwhile, detection sensitivity specificity EV-derived higher than those conventional serum biomarkers. In recent years, EVs been expected to become new marker biopsy. This review summarizes progress several aspects including sorting mechanisms, diagnostic value, technology isolation addition, study reviews challenges future research avenues field providing basis application miRNAs disease clinical diagnosis even point-of-care testing (POCT) platforms.

Language: Английский

Citations

71
Exosomes and Their Bioengineering Strategies in the Cutaneous Wound Healing and Related Complications: Current Knowledge and Future Perspectives DOI Creative Commons
Guang Yang,

Saquib Waheed,

Cong Wang

et al.

International Journal of Biological Sciences, Journal Year: 2023, Volume and Issue: 19(5), P. 1430 - 1454

Published: Jan. 1, 2023

Exosomes, as therapeutically relevant cell-secreted extracellular vesicles, have attracted enormous interest because they participate in intercellular communication and facilitate wound healing.Stem cell-derived exosomes exhibit similar biological effects to source cells with the exception of low immunogenicity no tumorigenicity, well superior efficacy promoting healing.Exosomes accelerate healing by angiogenesis cell proliferation, balancing inflammatory responses.Particularly, when are genetically modified or used combination materials, can better comprehensive therapeutic properties, such enriching active ingredients, targeted delivery, physiological barrier penetration, which not available traditional single products.Besides, also been considered for diagnostic uses related wounds, repairing complex enhancing graft success, treating complications, serving biomarkers.However, their clinical applications still face challenges, reliable commercial products yet available.This review will focus on recent research advances that describe characteristics isolation exosomes, introduce sources suitable repair illustrate value engineered development directions future, provide evidence potential application healing, discuss risks, solutions future applications.

Language: Английский

Citations

52
Extracellular vesicles in nanomedicine and regenerative medicine: A review over the last decade DOI Creative Commons
Saeid Moghassemi, Arezoo Dadashzadeh, Maria João Sousa

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 36, P. 126 - 156

Published: March 2, 2024

Small extracellular vesicles (sEVs) are known to be secreted by a vast majority of cells. These sEVs, specifically exosomes, induce specific cell-to-cell interactions and can activate signaling pathways in recipient cells through fusion or interaction. nanovesicles possess several desirable properties, making them ideal for regenerative medicine nanomedicine applications. properties include exceptional stability, biocompatibility, wide biodistribution, minimal immunogenicity. However, the practical utilization particularly clinical settings at large scale, is hindered expensive procedures required their isolation, limited circulation lifetime, suboptimal targeting capacity. Despite these challenges, sEVs have demonstrated remarkable ability accommodate various cargoes found extensive applications biomedical sciences. To overcome limitations broaden potential applications, researchers should strive deepen understanding current loading, characterization techniques. Additionally, acquiring fundamental knowledge about origins employing state-of-the-art methodologies expand research scope. This review provides comprehensive overview exosome-based strategies diverse domains, encompassing cancer therapy, immunotherapy, biomarker Furthermore, we emphasize immense exosomes medicine.

Language: Английский

Citations

32