Proteomic Profiling of Fallopian Tube-Derived Extracellular Vesicles Using a Microfluidic Tissue-on-Chip System DOI Creative Commons
Didi Zha, Sagar Rayamajhi, Jared Sipes

et al.

Bioengineering, Journal Year: 2023, Volume and Issue: 10(4), P. 423 - 423

Published: March 27, 2023

The human fallopian tube epithelium (hFTE) is the site of fertilization, early embryo development, and origin most high-grade serous ovarian cancers (HGSOCs). Little known about content functions hFTE-derived small extracellular vesicles (sEVs) due to limitations biomaterials proper culture methods. We have established a microfluidic platform hFTE for EV collection with adequate yield mass spectrometry-based proteomic profiling, reported 295 common sEV proteins first time. These are associated exocytosis, neutrophil degranulation, wound healing, some crucial fertilization processes. In addition, by correlating protein profiles tissue transcripts characterized using GeoMx® Cancer Transcriptome Atlas, spatial transcriptomics analysis revealed cell-type-specific that encode sEVs proteins, among which, FLNA, TUBB, JUP, FLNC were differentially expressed in secretory cells, precursor cells HGSOC. Our study provides insights into establishment baseline profile derived from tissue, its correlation lineage-specific transcripts, which can be used evaluate whether shifts cargo during cancer carcinogenesis role reproductive functions.

Language: Английский

Extracellular Vesicles as Therapeutic Resources in the Clinical Environment DOI Open Access
Jorge Sanz‐Ros, Cristina Mas‐Bargues, Nekane Romero-García

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 2344 - 2344

Published: Jan. 25, 2023

The native role of extracellular vesicles (EVs) in mediating the transfer biomolecules between cells has raised possibility to use them as therapeutic vehicles. development therapies based on EVs is now expanding rapidly; here we will describe current knowledge different key points regarding a clinical setting. These are related cell sources EVs, isolation, storage, and delivery methods, well modifications releasing for improved production EVs. Finally, depict application trials, considering impact COVID-19 pandemic these therapies, pointing out that although it promising therapy human diseases, still initial phase its patients.

Language: Английский

Citations

53
Migrasomes, a new mode of intercellular communication DOI Creative Commons

Yuyun Jiang,

Xi Liu,

Jixian Ye

et al.

Cell Communication and Signaling, Journal Year: 2023, Volume and Issue: 21(1)

Published: May 8, 2023

Abstract Migrasomes are newly discovered extracellular vesicles (EVs) that formed in migrating cells and mediate intercellular communication. However, their size, biological generation, cargo packaging, transport, effects on recipient by migrasomes different from those of other EVs. In addition to mediating organ morphogenesis during zebrafish gastrulation, discarding damaged mitochondria, lateral transport mRNA proteins, growing evidence has demonstrated a variety pathological processes. this review, we summarize the discovery, mechanisms formation, isolation, identification, mediation cellular communication migrasomes. We discuss migrasome-mediated disease processes, such as osteoclast differentiation, proliferative vitreoretinopathy, tumor cell metastasis PD-L1 immune chemotaxis site infection chemokines, angiogenesis promotion via angiogenic factors cells, leukemic mesenchymal stromal cells. Moreover, new EVs, propose potential for diagnosis treatment.

Language: Английский

Citations

48
Metabolic Messengers: small extracellular vesicles DOI
Theresa V. Rohm,

Karina Cunha e Rocha,

Jerrold M. Olefsky

et al.

Nature Metabolism, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

Language: Английский

Citations

4
Emerging Strategies for Revascularization: Use of Cell-Derived Extracellular Vesicles and Artificial Nanovesicles in Critical Limb Ischemia DOI Creative Commons

Vijay Murali Ravi Mythili,

Ramya Lakshmi Rajendran, Arun Renganathan

et al.

Bioengineering, Journal Year: 2025, Volume and Issue: 12(1), P. 92 - 92

Published: Jan. 20, 2025

Critical limb ischemia (CLI) poses a substantial and intricate challenge in vascular medicine, necessitating the development of innovative therapeutic strategies to address its multifaceted pathophysiology. Conventional revascularization approaches often fail adequately complexity CLI, identification alternative methodologies. This review explores uncharted territory beyond traditional therapies, focusing on potential two distinct yet interrelated entities: cell-derived extracellular vesicles (EVs) artificial nanovesicles. Cell-derived EVs are small membranous structures naturally released by cells, nanovesicles artificially engineered nanosized vesicles. Both these represent promising avenues for intervention. They act as carriers bioactive cargo, including proteins, nucleic acids, lipids, that can modulate cellular responses associated with ischemic tissue repair angiogenesis. also assesses evolving landscape CLI through unique perspective The spans spectrum from early preclinical investigations latest translational advancements, providing comprehensive overview current state research this emerging field. These groundbreaking vesicle therapies hold immense revolutionizing treatment paradigms.

Language: Английский

Citations

2
Dapagliflozin‐Loaded Exosome Mimetics Facilitate Diabetic Wound Healing by HIF‐1α‐Mediated Enhancement of Angiogenesis DOI Creative Commons
Weiyue Zhang, Lutong Wang, Haoyu Guo

et al.

Advanced Healthcare Materials, Journal Year: 2022, Volume and Issue: 12(7)

Published: Nov. 29, 2022

Angiogenesis plays a critical role in diabetic wound healing. However, no effective strategies have been developed to target endothelial cells (ECs) facilitate Dapagliflozin (DA) as sodium-glucose linked transporter 2 (SGLT2) inhibitor, may promote neovascularization mice via HIF-1α-mediated enhancement of angiogenesis. Here, the bioinspired nanovesicles (NVs) prepared from induced pluripotent stem cells-derived ECs through an extrusion approach are reported, which can function exosome mimetics achieve targeted deliver DA. Abundant membrane C-X-C motif chemokine receptor 4 conferred EC-targeting ability these NVs and homology facilitated accumulation ECs. Furthermore, DA-loaded (iPSC)-EC angiogenesis healing by HIF-1α/VEGFA pathway. Taken together, this study indicated that targeting regulating be promising strategy for treatment

Language: Английский

Citations

41
Chiral Graphene Quantum Dots Enhanced Drug Loading into Small Extracellular Vesicles DOI
Youwen Zhang, Gaeun Kim, Yini Zhu

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(11), P. 10191 - 10205

Published: May 1, 2023

As nanoscale extracellular vesicles secreted by cells, small (sEVs) have enormous potential as safe and effective vehicles to deliver drugs into lesion locations. Despite promising advances with sEV-based drug delivery systems, there are still challenges loading sEVs, which hinder the clinical applications of sEVs. Herein, we report an exogenous drug-agnostic chiral graphene quantum dots (GQDs) sEV-loading platform, based on chirality matching sEV lipid bilayer. Both hydrophobic hydrophilic chemical biological can be functionalized or adsorbed onto GQDs π–π stacking van der Waals interactions. By tuning ligands GQD size optimize its chirality, demonstrate efficiency 66.3% 64.1% for doxorubicin siRNA, is significantly higher than other reported techniques.

Language: Английский

Citations

37
Glycosylation in extracellular vesicles: Isolation, characterization, composition, analysis and clinical applications DOI Creative Commons

Veronika Vrablova,

Natália Košútová, Anna Blšáková

et al.

Biotechnology Advances, Journal Year: 2023, Volume and Issue: 67, P. 108196 - 108196

Published: June 10, 2023

This review provides a comprehensive overview of our understanding the role that glycans play in formation, loading and release extracellular vesicles (EVs). The capture EVs (typically with size 100-200 nm) is described, including approaches based on glycan recognition glycan-based analysis offering highly sensitive detection EVs. Furthermore, detailed information provided about use EV processing enzymes as potential biomarkers, therapeutic targets or tools applied for regenerative medicine. also short introduction into advanced methods characterization EVs, new insights biomolecular corona covering bioanalytical available analysis.

Language: Английский

Citations

27
CRISPR-Cas9 delivery strategies with engineered extracellular vesicles DOI Creative Commons
Yaoyao Lu, Kelly Godbout, Gabriel Lamothe

et al.

Molecular Therapy — Nucleic Acids, Journal Year: 2023, Volume and Issue: 34, P. 102040 - 102040

Published: Sept. 26, 2023

Therapeutic genome editing has the potential to cure diseases by directly correcting genetic mutations in tissues and cells. Recent progress CRISPR-Cas9 systems led breakthroughs gene tools because of its high orthogonality, versatility, efficiency. However, safe effective administration target organs patients is a major hurdle. Extracellular vesicles (EVs) are endogenous membranous particles secreted spontaneously all They key actors cell-to-cell communication, allowing exchange select molecules such as proteins, lipids, RNAs induce functional changes recipient Recently, EVs have displayed their for trafficking system during or after formation. In this review, we highlight recent developments EV loading, surface functionalization, strategies increasing efficiency delivering tissues, organs, cells eventual use therapies.

Language: Английский

Citations

27
MMP-9 as Prognostic Marker for Brain Tumours: A Comparative Study on Serum-Derived Small Extracellular Vesicles DOI Open Access
Gabriella Dobra, Edina Gyukity‐Sebestyen, Mátyás Bukva

et al.

Cancers, Journal Year: 2023, Volume and Issue: 15(3), P. 712 - 712

Published: Jan. 24, 2023

Matrix metalloproteinase-9 (MMP-9) degrades the extracellular matrix, contributes to tumour cell invasion and metastasis, its elevated level in brain tissues indicates poor prognosis. High-risk tissue biopsy can be replaced by liquid biopsy; however, blood-brain barrier (BBB) prevents tumour-associated components from entering peripheral blood, making development of blood-based biomarkers challenging. Therefore, we examined MMP-9 content small vesicles (sEVs)-which cross BBB are stable body fluids-to characterise tumours with different capacity. From four patient groups (glioblastoma multiforme, metastases lung cancer, meningioma, lumbar disc herniation as controls), 222 serum-derived sEV samples were evaluated. After isolating characterising sEVs, their was measured ELISA assessed statistically (correlation, paired t-test, Welch's test, ANOVA, ROC). We found that sEVs is independent gender age, but affected surgical intervention, treatment, recurrence. a relation between low (<28 ppm) improved survival (8-month advantage) glioblastoma patients, levels showed positive correlation aggressiveness. These findings suggest vesicular might useful prognostic marker for tumours.

Language: Английский

Citations

26
DNA walker induced “signal on” fluorescence aptasensor strategy for rapid and sensitive detection of extracellular vesicles in gastric cancer DOI

Gaojian Yang,

Zhiyang Li, Rabia Usman

et al.

Chinese Chemical Letters, Journal Year: 2024, Volume and Issue: 36(2), P. 109930 - 109930

Published: April 29, 2024

Language: Английский

Citations

10