Advances in Super-resolution Stimulated Raman Scattering Microscopy DOI Creative Commons
William J. Tipping, Karen Faulds, Duncan Graham

et al.

Chemical & Biomedical Imaging, Journal Year: 2024, Volume and Issue: 2(11), P. 733 - 743

Published: Oct. 4, 2024

Super-resolution optical imaging overcomes the diffraction limit in light microscopy to enable visualization of previously invisible molecular details within a sample. The realization super-resolution based on stimulated Raman scattering (SRS) represents recent area fruitful development that has been used visualize cellular structures three dimensions, with multiple spectroscopic colors at nanometer scale. Several fundamental approaches achieving SRS have reported, including engineering strategies, expansion microscopy, deconvolution image analysis, and photoswitchable reporters as methods break limit. These enabled biological structures, interactions, dynamics unprecedented detail. In this Perspective, an overview current strategies capabilities for will be highlighted together outlook potential directions rapidly evolving field.

Language: Английский

Virtual formalin-fixed and paraffin-embedded staining of fresh brain tissue via stimulated Raman CycleGAN model DOI Creative Commons
Zhijie Liu, Lingchao Chen, Haixia Cheng

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(13)

Published: March 27, 2024

Intraoperative histology is essential for surgical guidance and decision-making. However, frozen-sectioned hematoxylin eosin (H&E) staining suffers from degraded accuracy, whereas the gold-standard formalin-fixed paraffin-embedded (FFPE) H&E too lengthy intraoperative use. Stimulated Raman scattering (SRS) microscopy has shown rapid of brain tissue with lipid/protein contrast but challenging to yield images identical nucleic acid-/protein-based FFPE stains interpretable pathologists. Here, we report development a semi-supervised stimulated CycleGAN model convert fresh-tissue SRS using unpaired training data. Within 3 minutes, virtual (SRVH) results that matched perfectly true could be generated. A blind validation indicated board-certified neuropathologists are able differentiate histologic subtypes human glioma on SRVH hardly conventional images. may provide diagnosis superior frozen in both speed extendable other types solid tumors.

Language: Английский

Citations

30
Label-free nanoscopy of cell metabolism by ultrasensitive reweighted visible stimulated Raman scattering DOI
Haonan Lin,

Scott Seitz,

Yuying Tan

et al.

Nature Methods, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

Language: Английский

Citations

2
Viewing life without labels under optical microscopes DOI Creative Commons
Biswajoy Ghosh, Krishna Agarwal

Communications Biology, Journal Year: 2023, Volume and Issue: 6(1)

Published: May 25, 2023

Abstract Optical microscopes today have pushed the limits of speed, quality, and observable space in biological specimens revolutionizing how we view life today. Further, specific labeling samples for imaging has provided insight into functions. This enabled label-based microscopy to percolate integrate mainstream science research. However, use labelfree been mostly limited, resulting testing bio-application but not bio-integration. To enable bio-integration, such need be evaluated their timeliness answer questions uniquely establish a long-term growth prospect. The article presents key label-free optical discusses integrative potential research unperturbed analysis samples.

Language: Английский

Citations

30
Rational Design and Engineering of Conducting Polymers for Chemical Enhancement in Raman Scattering DOI Open Access
Huanhuan Zhang, J. Dong,

Xuke Tang

et al.

Advanced Optical Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 30, 2025

Abstract Raman scattering is characterized by the inherently weak inelastic of photons, influenced molecular vibrations or rotations. Recent advances have shifted from traditional electromagnetic enhancement methods to chemically enhanced scattering, offering significant advantages. However, these advancements typically depended on indirect and empirical models. This article introduces a systematic method for rational design engineering chemical scattering. involves identifying promising enhancers optimizing their morphology composition elucidating photochemical properties mapping charge‐transfer pathways with target molecules using transient absorption spectroscopy (TAS), cyclic voltammetry (CV), density functional theory (DFT) calculations. Employing this method, work has developed series rationally designed made conducting polymers (CPs), such as poly(3,4‐ethylenedioxythiophene) (PEDOT), optimized morphological traits compositions. These significantly improve surface‐enhanced (SERS), achieving reproducible factor up 10 6 , boost lasing, remarkable 40‐fold increase in energy conversion efficiency.

Language: Английский

Citations

1
Far-field super-resolution chemical microscopy DOI Creative Commons
Mingwei Tang, Yubing Han, Danchen Jia

et al.

Light Science & Applications, Journal Year: 2023, Volume and Issue: 12(1)

Published: June 5, 2023

Far-field chemical microscopy providing molecular electronic or vibrational fingerprint information opens a new window for the study of three-dimensional biological, material, and systems. Chemical provides nondestructive way identification without exterior labels. However, diffraction limit optics hindered it from discovering more details under resolution limit. Recent development super-resolution techniques gives enlightenment to open this door behind far-field microscopy. Here, we review recent advances that have pushed boundary in terms spatial resolution. We further highlight applications biomedical research, material characterization, environmental study, cultural heritage conservation, integrated chip inspection.

Language: Английский

Citations

15
Bioorthogonal Stimulated Raman Scattering Imaging Uncovers Lipid Metabolic Dynamics in Drosophila Brain During Aging DOI
Yajuan Li,

Phyllis Chang,

Shriya Sankaran

et al.

GEN Biotechnology, Journal Year: 2023, Volume and Issue: 2(3), P. 247 - 261

Published: June 1, 2023

Studies have shown that brain lipid metabolism is associated with biological aging and influenced by dietary genetic manipulations; however, the underlying mechanisms are elusive. High-resolution imaging techniques propose a novel potent approach to understanding metabolic dynamics in situ. Applying deuterium water (D2O) probing stimulated Raman scattering (DO-SRS) microscopy, we revealed activity Drosophila decreased sex-dependent manner. Female flies showed an earlier occurrence of turnover decrease than males. Dietary restriction (DR) downregulation insulin/IGF-1 signaling (IIS) pathway, two scenarios for lifespan extension, led significant enhancements old flies. Combining SRS deuterated bioorthogonal probes (deuterated glucose acetate), discovered that, under DR treatment IIS shifted use acetate as major carbon source synthesis. For first time, our study directly visualizes quantifies spatiotemporal alterations at single organelle (lipid droplet) level. Our not only demonstrates new studying situ but also illuminates interconnection aging, dietary, manipulations on regulation.

Language: Английский

Citations

14
Integrative spatial protein profiling with multi-omics DOI
Rong Fan

Nature Methods, Journal Year: 2024, Volume and Issue: 21(12), P. 2223 - 2225

Published: Dec. 1, 2024

Language: Английский

Citations

6
Stain-Free Histopathology with Stimulated Raman Scattering Microscopy DOI
Liyang Ma, Kuan Luo, Zhijie Liu

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(20), P. 7907 - 7925

Published: May 7, 2024

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTStain-Free Histopathology with Stimulated Raman Scattering MicroscopyLiyang MaLiyang MaState Key Laboratory of Surface Physics and Department Physics, Academy for Engineering Technology, Micro Nano Photonic Structures (Ministry Education), Shanghai Metasurfaces Light Manipulation, Fudan University, 200433, ChinaMore by Liyang MaView Biographyhttps://orcid.org/0009-0007-8820-1403, Kuan LuoKuan LuoState LuoView Biographyhttps://orcid.org/0009-0001-2661-1577, Zhijie LiuZhijie LiuState LiuView Biography, Minbiao Ji*Minbiao JiState China*Email: [email protected]More JiView Biographyhttps://orcid.org/0000-0002-9066-4008Cite this: Anal. Chem. 2024, 96, 20, 7907–7925Publication Date (Web):May 7, 2024Publication History Received20 April 2024Accepted24 2024Revised23 2024Published online7 May inissue 21 2024https://pubs.acs.org/doi/10.1021/acs.analchem.4c02061https://doi.org/10.1021/acs.analchem.4c02061review-articleACS PublicationsCopyright © 2024 American Chemical SocietyRequest reuse permissionsArticle Views425Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated to reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com additional details score social media presence given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Biological imaging,Cancer,Central nervous system,Microscopy,Raman scattering Get e-Alerts

Language: Английский

Citations

4
Multimodal imaging of metabolic activities for distinguishing subtypes of breast cancer DOI Creative Commons
Zhi Li,

Chloe Nguyen,

Hongje Jang

et al.

Biomedical Optics Express, Journal Year: 2023, Volume and Issue: 14(11), P. 5764 - 5764

Published: Sept. 28, 2023

Triple negative breast cancer (TNBC) is a highly aggressive form of cancer. Detecting TNBC early crucial for improving disease prognosis and optimizing treatment. Unfortunately, conventional imaging techniques fall short in providing comprehensive differentiation subtypes due to their limited sensitivity inability capture subcellular details. In this study, we present multimodal platform that integrates heavy water (D 2 O)-probed stimulated Raman scattering (DO-SRS), two-photon fluorescence (TPF), second harmonic generation (SHG) imaging. This allows us directly visualize quantify the metabolic activities at level. By utilizing DO-SRS imaging, were able identify distinct levels de novo lipogenesis, protein synthesis, cytochrome c heterogeneity, lipid unsaturation rates various subtype tissues. Simultaneously, TPF provided spatial distribution mapping NAD[P]H flavin signals tissues, revealing high redox ratio significant turnover rate BL2 (HCC1806) samples. Furthermore, SHG enabled observe diverse orientations collagen fibers with higher anisotropy tissue boundary compared center. Our offers sensitive approach characterizing not only TNBC, but also other cancers.

Language: Английский

Citations

10
Rapid Wide-Field Correlative Mapping of Electronic and Vibrational Ultrafast Dynamics in Solids DOI Creative Commons
Rihan Wu, Yaqing Zhang, Md. Shahjahan

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Coupling between electronic and vibrational degrees of freedom in solids is responsible for many fundamental material properties, including superconductivity, ferroelectricity, high thermal conductivity, indirect bandgap emission among others. In heterogeneous materials electronic-vibrational coupling gives rise to spatial correlations the relaxation properties dynamics. Visualizing mapping these may lead important physical insights applications that include electronics, optoelectronics, energy technologies. However, due vastly different scales involved, measuring correlating challenging. While principle, ultrafast pulses with sufficient bandwidth generate excited-state population coherence signatures, need measure signal point-by-point across sample results relatively slow acquisition, leading an increased risk photodamage rendering measurements highly susceptible noise. Here, we introduce Parallel Rapid Imaging Spectroscopic Mapping (PRISM), ultrafast, wide-field, coherent imaging technique, allowed simultaneous acquisition state decay 0–10 ps range spectra structurally sensitive low-frequency 5–600 cm–1 range. The exceptionally speed PRISM, exceeding 1.6 million time-resolved traces per second, enabled 80,000 pixels simultaneously few-layer tungsten diselenide perovskite materials. Correlations maps reveal heterogeneity not observed by either measurement alone. ability map makes PRISM particularly well-suited studies complex a wide applications.

Language: Английский

Citations

0