Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: July 26, 2024
Fast
electrical
signaling
in
dendrites
is
central
to
neural
computations
that
support
adaptive
behaviors.
Conventional
techniques
lack
temporal
and
spatial
resolution
the
ability
track
underlying
membrane
potential
dynamics
present
across
complex
three-dimensional
dendritic
arbor
vivo.
Here,
we
perform
fast
two-photon
imaging
of
somatic
single
pyramidal
cells
CA1
region
mouse
hippocampus
during
awake
behavior.
We
study
subthreshold
suprathreshold
events
throughout
vivo
by
combining
voltage
with
simultaneous
local
field
recording,
post
hoc
morphological
reconstruction,
a
navigation
task.
systematically
quantify
modulation
event
rates
locomotion
distinct
regions,
report
an
advancing
gradient
theta
phase
along
basal-tuft
axis,
describe
predominant
hyperpolarization
sharp-wave
ripples.
Finally,
find
tuning
representations
dynamically
reorganizes
following
place
formation.
Our
data
reveal
how
organization
maps
onto
anatomy
tree
behavior,
oscillatory
network,
functional
cell
states.
Neurons
receive
their
input
three
dimensions
via
dendrites,
but
activity
organized
unknown.
authors
work
out
rules
govern
this
3D
structure
different
brain
Nature Methods,
Journal Year:
2023,
Volume and Issue:
20(10), P. 1581 - 1592
Published: Sept. 18, 2023
Abstract
Here
we
report
SUPPORT
(statistically
unbiased
prediction
utilizing
spatiotemporal
information
in
imaging
data),
a
self-supervised
learning
method
for
removing
Poisson–Gaussian
noise
voltage
data.
is
based
on
the
insight
that
pixel
value
data
highly
dependent
its
neighboring
pixels,
even
when
temporally
adjacent
frames
alone
do
not
provide
useful
statistical
prediction.
Such
dependency
captured
and
used
by
convolutional
neural
network
with
blind
spot
to
accurately
denoise
which
existence
of
action
potential
time
frame
cannot
be
inferred
other
frames.
Through
simulations
experiments,
show
enables
precise
denoising
types
microscopy
image
while
preserving
underlying
dynamics
within
scene.
Nature Computational Science,
Journal Year:
2023,
Volume and Issue:
3(12), P. 1067 - 1080
Published: Dec. 11, 2023
Abstract
Fluorescence
imaging
with
high
signal-to-noise
ratios
has
become
the
foundation
of
accurate
visualization
and
analysis
biological
phenomena.
However,
inevitable
noise
poses
a
formidable
challenge
to
sensitivity.
Here
we
provide
spatial
redundancy
denoising
transformer
(SRDTrans)
remove
from
fluorescence
images
in
self-supervised
manner.
First,
sampling
strategy
based
on
is
proposed
extract
adjacent
orthogonal
training
pairs,
which
eliminates
dependence
speed.
Second,
designed
lightweight
spatiotemporal
architecture
capture
long-range
dependencies
high-resolution
features
at
low
computational
cost.
SRDTrans
can
restore
high-frequency
information
without
producing
oversmoothed
structures
distorted
traces.
Finally,
demonstrate
state-of-the-art
performance
single-molecule
localization
microscopy
two-photon
volumetric
calcium
imaging.
does
not
contain
any
assumptions
about
process
sample,
thus
be
easily
extended
various
modalities
applications.
Abstract
Ultrafast
3D
imaging
is
indispensable
for
visualizing
complex
and
dynamic
biological
processes.
Conventional
scanning-based
techniques
necessitate
an
inherent
trade-off
between
acquisition
speed
space-bandwidth
product
(SBP).
Emerging
single-shot
wide-field
offer
a
promising
alternative
but
are
bottlenecked
by
the
synchronous
readout
constraints
of
conventional
CMOS
systems,
thus
restricting
data
throughput
to
maintain
high
SBP
at
limited
frame
rates.
To
address
this,
we
introduce
EventLFM,
straightforward
cost-effective
system
that
overcomes
these
challenges
integrating
event
camera
with
Fourier
light
field
microscopy
(LFM),
state-of-the-art
technique.
The
operates
on
novel
asynchronous
architecture,
thereby
bypassing
rate
limitations
systems.
We
further
develop
simple
robust
event-driven
LFM
reconstruction
algorithm
can
reliably
reconstruct
dynamics
from
unique
spatiotemporal
measurements
captured
EventLFM.
Experimental
results
demonstrate
EventLFM
robustly
fast-moving
rapidly
blinking
fluorescent
samples
kHz
Furthermore,
highlight
EventLFM’s
capability
neuronal
signals
in
scattering
mouse
brain
tissues
tracking
GFP-labeled
neurons
freely
moving
C.
elegans
.
believe
combined
ultrafast
large
offered
may
open
up
new
possibilities
across
many
biomedical
applications.
Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: June 14, 2024
Abstract
Two-photon
voltage
imaging
has
long
been
heralded
as
a
transformative
approach
capable
of
answering
many
long-standing
questions
in
modern
neuroscience.
However,
exploiting
its
full
potential
requires
the
development
novel
approaches
well
suited
to
photophysical
properties
genetically
encoded
indicators.
We
demonstrate
that
parallel
excitation
developed
for
scanless
two-photon
photostimulation
enable
high-SNR
imaging.
use
whole-cell
patch-clamp
electrophysiology
perform
thorough
characterization
using
three
illumination
and
lasers
with
different
repetition
rates
wavelengths.
recordings
high-frequency
spike
trains
sub-threshold
depolarizations
from
neurons
expressing
soma-targeted
indicator
JEDI-2P-Kv.
Using
low
repetition-rate
laser,
we
multi-cell
up
fifteen
targets
simultaneously.
co-express
JEDI-2P-Kv
channelrhodopsin
ChroME-ST
capitalize
on
their
overlapping
absorption
spectra
simultaneously
evoke
image
action
potentials
single
laser
source.
also
vivo
multiple
cells
250
µm
deep
barrel
cortex
head-fixed,
anaesthetised
mice.
Neuroscience Research,
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 1, 2024
Monitoring
neural
activity
in
individual
neurons
is
crucial
for
understanding
circuits
and
brain
functions.
The
emergence
of
optical
imaging
technologies
has
dramatically
transformed
the
field
neuroscience,
enabling
detailed
observation
large-scale
neuronal
populations
with
both
cellular
subcellular
resolution.
This
transformation
will
be
further
accelerated
by
integration
these
advanced
big
data
analysis.
Genetically
encoded
fluorescent
indicators
to
detect
high
signal-to-noise
ratios
are
pivotal
this
advancement.
In
recent
years,
have
undergone
significant
developments,
greatly
enhancing
dynamics
networks.
review
highlights
progress
genetically
calcium
voltage
discusses
future
direction
techniques
analysis
that
deepens
our
complexities
brain.
Optica,
Journal Year:
2023,
Volume and Issue:
10(6), P. 763 - 763
Published: April 26, 2023
High-speed
laser
scanning
microscopes
are
essential
for
monitoring
fast
biological
phenomena.
However,
existing
strategies
that
achieve
millisecond
time
resolution
with
two-photon
(2PMs)
generally
technically
challenging
and
suffer
from
compromises
among
imaging
field
of
view,
excitation
efficiency,
depth
penetration
in
thick
tissue.
Here,
we
present
a
versatile
solution
enables
conventional
video-rate
2PM
to
perform
2D
at
kilohertz
frame
rates
over
large
fields
view.
Our
system
is
based
on
implementation
scan
multiplier
unit
provides
inertia-free
multiplication
the
speed
while
preserving
all
benefits
standard
2PM.
We
demonstrate
subcellular-resolution
an
order
magnitude
higher
throughput
than
previously
achievable
depths
exceeding
500
µm,
which
apply
study
neurovascular
coupling
dynamics
mouse
brain.
