FEBS Journal,
Journal Year:
2022,
Volume and Issue:
289(21), P. 6463 - 6483
Published: Jan. 24, 2022
Regeneration
of
the
mammalian
adult
skeletal
muscle
is
a
well‐orchestrated
process
regulated
by
multiple
proteins
and
signalling
pathways.
Cytokines
constitute
major
class
regulators
myogenesis.
It
well
established
that
infiltrating
immune
cells
at
site
injury
secrete
cytokines,
which
play
critical
roles
in
myofibre
repair
regeneration
process.
In
past
10–15
years,
itself
has
emerged
as
prolific
producer
cytokines.
Much
attention
field
been
focused
on
endocrine
effects
muscle‐secreted
cytokines
(myokines)
metabolic
regulation.
However,
ample
evidence
suggests
muscle‐derived
also
regulate
myogenic
differentiation
an
autocrine
manner.
this
review,
we
survey
meet
two
criteria:
(a)
expression
cells;
(b)
demonstrating
function.
Dozens
representing
several
classes
make
up
group,
together
they
all
steps
How
such
large
array
coordinate
their
to
form
regulatory
network
fascinating,
pressing
question.
Functional
studies
can
distinguish
source
vivo
are
much
needed
order
facilitate
exploration
full
therapeutic
potential.
Signal Transduction and Targeted Therapy,
Journal Year:
2023,
Volume and Issue:
8(1)
Published: Jan. 16, 2023
Abstract
Clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)/CRISPR-associated
protein
9
(Cas9)
gene-editing
technology
is
the
ideal
tool
of
future
for
treating
diseases
by
permanently
correcting
deleterious
base
mutations
or
disrupting
disease-causing
genes
with
great
precision
and
efficiency.
A
variety
efficient
Cas9
variants
derivatives
have
been
developed
to
cope
complex
genomic
changes
that
occur
during
diseases.
However,
strategies
effectively
deliver
CRISPR
system
diseased
cells
in
vivo
are
currently
lacking,
nonviral
vectors
target
recognition
functions
may
be
focus
research.
Pathological
physiological
resulting
from
disease
onset
expected
serve
as
identifying
factors
targeted
delivery
targets
gene
editing.
Diseases
both
varied
complex,
choice
appropriate
methods
different
important.
Meanwhile,
there
still
many
potential
challenges
identified
when
targeting
CRISPR/Cas9
treatment.
This
paper
reviews
current
developments
three
aspects,
namely,
type,
vector,
characteristics.
Additionally,
this
summarizes
successful
examples
clinical
trials
finally
describes
possible
problems
associated
applications.
Journal of Molecular Biology,
Journal Year:
2018,
Volume and Issue:
431(1), P. 34 - 47
Published: June 26, 2018
Programmable
control
of
gene
expression
is
essential
to
understanding
function,
engineering
cellular
behaviors,
and
developing
therapeutics.
Beyond
the
editing
applications
enabled
by
nuclease
CRISPR-Cas9
CRISPR-Cas12a,
invention
nuclease-dead
Cas
molecules
(dCas9
dCas12a)
offers
a
platform
for
precise
genome
function
without
editing.
Diverse
dCas
tools
have
been
developed,
which
constitute
comprehensive
toolbox
that
allows
interrogation
modulation
behaviors.
This
review
summarizes
current
transcription
regulation,
epigenetic
engineering,
imaging,
genetic
screens,
chromatin
immunoprecipitation.
We
also
highlight
advantages
existing
challenges
in
synthetic
biology,
provide
perspectives
on
future
directions
applications.
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2020,
Volume and Issue:
8
Published: March 24, 2020
Exploring
innovative
solutions
to
improve
the
healthcare
of
aging
and
diseased
population
continues
being
a
global
challenge.
Among
number
strategies
toward
this
goal,
tissue
engineering
regenerative
medicine
(TERM)
has
gradually
evolved
into
promising
approach
meet
future
needs
patients.
TERM
recently
received
increasing
attention
in
Asia,
as
evidenced
by
markedly
increased
researchers,
publications,
clinical
trials
translational
products.
This
review
aims
give
brief
overview
development
Asia
over
last
decade
highlighting
some
important
advances
field
featuring
major
achievements
representative
research
groups.
The
novel
biomaterials
enabling
technologies,
identification
new
cell
sources,
applications
various
tissues
are
briefly
introduced.
Finally,
achievement
including
discoveries,
examples
commercial
products
will
be
Discussion
on
current
limitations
directions
hot
also
provided.
Nature,
Journal Year:
2024,
Volume and Issue:
628(8006), P. 154 - 161
Published: March 13, 2024
Abstract
Several
genetic
risk
factors
for
Alzheimer’s
disease
implicate
genes
involved
in
lipid
metabolism
and
many
of
these
are
highly
expressed
glial
cells
1
.
However,
the
relationship
between
glia
pathology
remains
poorly
understood.
Through
single-nucleus
RNA
sequencing
brain
tissue
disease,
we
have
identified
a
microglial
state
defined
by
expression
droplet-associated
enzyme
ACSL1
with
ACSL1-positive
microglia
being
most
abundant
patients
having
APOE4/4
genotype.
In
human
induced
pluripotent
stem
cell-derived
microglia,
fibrillar
Aβ
induces
expression,
triglyceride
synthesis
droplet
accumulation
an
APOE-dependent
manner.
Additionally,
conditioned
media
from
droplet-containing
lead
to
Tau
phosphorylation
neurotoxicity
Our
findings
suggest
link
neurotoxic
microglia-derived
factors,
potentially
providing
therapeutic
strategies
disease.
Cell Reports,
Journal Year:
2019,
Volume and Issue:
26(5), P. 1174 - 1188.e5
Published: Jan. 1, 2019
Neuronal
activity-inducible
gene
transcription
correlates
with
rapid
and
transient
increases
in
histone
acetylation
at
promoters
enhancers
of
activity-regulated
genes.
Exactly
how
modulates
these
genes
has
remained
unknown.
We
used
single-cell
situ
transcriptional
analysis
to
show
that
Fos
Npas4
are
transcribed
stochastic
bursts
mouse
neurons
membrane
depolarization
mRNA
expression
by
increasing
burst
frequency.
then
expressed
dCas9-p300
or
dCas9-HDAC8
fusion
proteins
mimic
block
activity-induced
locally
enhancers.
Adding
increased
prolonging
duration
resulted
higher
protein
levels
an
elevation
resting
potential.
Inhibiting
reduced
reducing
frequency
impaired
experience-dependent
induction
the
hippocampus
vivo.
Thus,
tunes
dynamics
experience-regulated
affect
selective
changes
neuronal
cellular
function.
