Annual Review of Animal Biosciences,
Journal Year:
2020,
Volume and Issue:
9(1), P. 401 - 421
Published: Nov. 25, 2020
Unique,
functional,
homodimeric
heavy
chain-only
antibodies,
devoid
of
light
chains,
are
circulating
in
the
blood
Camelidae.
These
antibodies
recognize
their
cognate
antigen
via
one
single
domain,
known
as
VHH
or
Nanobody.
This
serendipitous
discovery
made
three
decades
ago
has
stimulated
a
growing
number
researchers
to
generate
highly
specific
Nanobodies
against
myriad
targets.
The
small
size,
strict
monomeric
state,
robustness,
and
easy
tailoring
these
have
inspired
many
groups
design
innovative
Nanobody-based
multi-domain
constructs
explore
novel
applications.
As
such,
been
employed
an
exquisite
research
tool
structural,
cell,
developmental
biology.
Furthermore,
demonstrated
benefit
for
more
sensitive
diagnostic
tests.
Finally,
several
designed
develop
new
therapeutic
products.
Nature Methods,
Journal Year:
2021,
Volume and Issue:
18(12), P. 1532 - 1541
Published: Nov. 4, 2021
Abstract
Imaging
intact
human
organs
from
the
organ
to
cellular
scale
in
three
dimensions
is
a
goal
of
biomedical
imaging.
To
meet
this
challenge,
we
developed
hierarchical
phase-contrast
tomography
(HiP-CT),
an
X-ray
phase
propagation
technique
using
European
Synchrotron
Radiation
Facility
(ESRF)’s
Extremely
Brilliant
Source
(EBS).
The
spatial
coherence
ESRF-EBS
combined
with
our
beamline
equipment,
sample
preparation
and
scanning
developments
enabled
us
perform
non-destructive,
three-dimensional
(3D)
scans
hierarchically
increasing
resolution
at
any
location
whole
organs.
We
applied
HiP-CT
image
five
types:
brain,
lung,
heart,
kidney
spleen.
provided
structural
overview
each
followed
by
multiple
higher-resolution
volumes
interest,
capturing
organotypic
functional
units
certain
individual
specialized
cells
within
demonstrate
potential
applications
through
quantification
morphometry
glomeruli
identification
regional
changes
tissue
architecture
lung
deceased
donor
coronavirus
disease
2019
(COVID-19).
Nature,
Journal Year:
2022,
Volume and Issue:
611(7936), P. 585 - 593
Published: Nov. 9, 2022
Macrophages
are
important
players
in
the
maintenance
of
tissue
homeostasis1.
Perivascular
and
leptomeningeal
macrophages
reside
near
central
nervous
system
(CNS)
parenchyma2,
their
role
CNS
physiology
has
not
been
sufficiently
well
studied.
Given
continuous
interaction
with
cerebrospinal
fluid
(CSF)
strategic
positioning,
we
refer
to
these
cells
collectively
as
parenchymal
border
(PBMs).
Here
demonstrate
that
PBMs
regulate
CSF
flow
dynamics.
We
identify
a
subpopulation
express
high
levels
CD163
LYVE1
(scavenger
receptor
proteins),
closely
associated
brain
arterial
tree,
show
LYVE1+
motion
drives
flow.
Pharmacological
or
genetic
depletion
led
accumulation
extracellular
matrix
proteins,
obstructing
access
perivascular
spaces
impairing
perfusion
clearance.
Ageing-associated
alterations
impairment
dynamics
were
restored
after
intracisternal
injection
macrophage
colony-stimulating
factor.
Single-nucleus
RNA
sequencing
data
obtained
from
patients
Alzheimer's
disease
(AD)
non-AD
individuals
point
changes
phagocytosis,
endocytosis
interferon-γ
signalling
on
PBMs,
pathways
corroborated
mouse
model
AD.
Collectively,
our
results
new
cellular
regulators
dynamics,
which
could
be
targeted
pharmacologically
alleviate
clearance
deficits
ageing
macrophages,
termed
here
shown
fluid,
implicating
this
cell
population
therapeutic
targets
neurological
diseases
such
Alzheimer's.
