Topological segregation of stress sensors along the gut crypt–villus axis DOI
Kouki K Touhara, Nathan D. Rossen, Fei Deng

et al.

Nature, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Language: Английский

Pushing the frontiers: tools for monitoring neurotransmitters and neuromodulators DOI
Zhaofa Wu, Dayu Lin, Yulong Li

et al.

Nature reviews. Neuroscience, Journal Year: 2022, Volume and Issue: 23(5), P. 257 - 274

Published: March 31, 2022

Language: Английский

Citations

159
Directed Evolution of a Selective and Sensitive Serotonin Sensor via Machine Learning DOI Creative Commons
Elizabeth K. Unger, Jacob P. Keller, Michael Altermatt

et al.

Cell, Journal Year: 2020, Volume and Issue: 183(7), P. 1986 - 2002.e26

Published: Dec. 1, 2020

Language: Английский

Citations

150
Psychedelic-inspired drug discovery using an engineered biosensor DOI Creative Commons
Chunyang Dong,

Calvin Ly,

Lee E. Dunlap

et al.

Cell, Journal Year: 2021, Volume and Issue: 184(10), P. 2779 - 2792.e18

Published: April 28, 2021

Language: Английский

Citations

145
Noradrenergic circuit control of non-REM sleep substates DOI Creative Commons
Alejandro Osorio-Forero, Romain Cardis, Gil Vantomme

et al.

Current Biology, Journal Year: 2021, Volume and Issue: 31(22), P. 5009 - 5023.e7

Published: Oct. 14, 2021

To understand what makes sleep vulnerable in disease, it is useful to look at how wake-promoting mechanisms affect healthy sleep. Wake-promoting neuronal activity inhibited during non-rapid-eye-movement (NREMS). However, sensory vigilance persists NREMS animals and humans, suggesting that wake promotion could remain functional. Here, we demonstrate consolidated mouse a brain state with recurrent fluctuations of the neurotransmitter noradrenaline on ∼50-s timescale thalamus. These occurred around mean levels greater than ones quiet wakefulness, while (NA) declined steeply REMS. They coincided clustering spindle rhythms forebrain heart-rate variations, both which are correlates arousability. We addressed origins these by using closed-loop optogenetic locus coeruleus (LC) activation or inhibition timed moments low high NREMS. suppress, lock, entrain sleep-spindle fore- hindbrain-projecting LC neurons show coordinated infraslow variations natural Noradrenergic modulation thalamic, but not cortical, circuits was required for involved NA release into primary reticular thalamic nuclei activated α1- β-adrenergic receptors cause slowly decaying membrane depolarizations. signaling constitutes vigilance-promoting mechanism renders mammalian disruption close-to-minute through sustaining thalamocortical autonomic arousability.Video abstracthttps://www.cell.com/cms/asset/ab9ec3b8-a3f1-470e-8a9c-a2d6ebebc742/mmc3.mp4Loading ...(mp4, 35.34 MB) Download video

Language: Английский

Citations

138
A sensitive GRAB sensor for detecting extracellular ATP in vitro and in vivo DOI Creative Commons
Zhaofa Wu, Kaikai He, Yue Chen

et al.

Neuron, Journal Year: 2021, Volume and Issue: 110(5), P. 770 - 782.e5

Published: Dec. 22, 2021

Language: Английский

Citations

133
A fluorescent sensor for spatiotemporally resolved imaging of endocannabinoid dynamics in vivo DOI
Ao Dong, Kaikai He, Barna Dudok

et al.

Nature Biotechnology, Journal Year: 2021, Volume and Issue: 40(5), P. 787 - 798

Published: Nov. 11, 2021

Language: Английский

Citations

132
Implantable aptamer–field-effect transistor neuroprobes for in vivo neurotransmitter monitoring DOI Creative Commons
Chuanzhen Zhao, Kevin M. Cheung,

Iwen Huang

et al.

Science Advances, Journal Year: 2021, Volume and Issue: 7(48)

Published: Nov. 24, 2021

Implantable aptamer transistor probes for in vivo neurotransmitter monitoring advance brain activity recording.

Language: Английский

Citations

113
A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility DOI Creative Commons
Shu‐Hsien Sheu, Srigokul Upadhyayula, Vincent Dupuy

et al.

Cell, Journal Year: 2022, Volume and Issue: 185(18), P. 3390 - 3407.e18

Published: Sept. 1, 2022

Chemical synapses between axons and dendrites mediate neuronal intercellular communication. Here, we describe a synapse primary cilia: the axo-ciliary synapse. Using enhanced focused ion beam-scanning electron microscopy on samples with optimally preserved ultrastructure, discovered brainstem serotonergic cilia of hippocampal CA1 pyramidal neurons. Functionally, these are enriched in ciliary-restricted serotonin receptor, 5-hydroxytryptamine receptor 6 (5-HTR6). cilia-targeted sensor, show that opto- chemogenetic stimulation releases onto cilia. Ciliary 5-HTR6 activates non-canonical G

Language: Английский

Citations

112
A genetically encoded sensor for in vivo imaging of orexin neuropeptides DOI
Loïc Duffet,

Seher Kosar,

Mariangela Panniello

et al.

Nature Methods, Journal Year: 2022, Volume and Issue: 19(2), P. 231 - 241

Published: Feb. 1, 2022

Language: Английский

Citations

94
A genetically encoded sensor measures temporal oxytocin release from different neuronal compartments DOI
Tongrui Qian, Huan Wang, Peng Wang

et al.

Nature Biotechnology, Journal Year: 2023, Volume and Issue: 41(7), P. 944 - 957

Published: Jan. 2, 2023

Language: Английский

Citations

82