Automating licking bias correction in a two-choice delayed match-to-sample task to accelerate learning DOI Creative Commons

Jongrok Do,

Min Whan Jung, Doyun Lee

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Dec. 20, 2023

Animals often display choice bias, or a preference for one option over the others, which can significantly impede learning new tasks. Delayed match-to-sample (DMS) tasks with two-alternative choices of lickports on left and right have been widely used to study sensory processing, working memory, associative memory in head-fixed animals. However, extensive training time, primarily due animals' biased licking responses, limits their practical utility. Here, we present implementation an automated side bias correction system olfactory DMS task, where lickport positions ratio left- right-rewarded trials are dynamically adjusted counterbalance mouse's responses during training. The algorithm moves preferred farther away from mouth non-preferred closer, while also increasing proportion when occurs. We found that adjusting distances proportions versus effectively reduces bias. Further analyses reveal these adjustments correlate subsequent improvements behavioral performance. Our findings suggest is valuable tool enhancing applicability involving choices.

Language: Английский

Activity map of a cortico-cerebellar loop underlying motor planning DOI Creative Commons
Jia Zhu,

Hana Hasanbegović,

Liu D. Liu

et al.

Nature Neuroscience, Journal Year: 2023, Volume and Issue: 26(11), P. 1916 - 1928

Published: Oct. 9, 2023

The neocortex and cerebellum interact to mediate cognitive functions. It remains unknown how the two structures organize into functional networks specific behaviors. Here we delineate activity supporting motor planning in relation mesoscale cortico-cerebellar connectome. In mice directional licking based on short-term memory, preparatory instructing future movement depends anterior lateral cortex (ALM) cerebellum. Transneuronal tracing revealed divergent largely open-loop connectivity between ALM distributed regions of A cerebellum-wide survey neuronal enriched hotspot with conjunctive input-output ALM. Perturbation experiments show that conjunction were required for maintaining correct subsequent movement. Other cerebellar contributed little despite input or output These results identify a loop suggest selectively establishes reciprocal communications orchestrate planning.

Language: Английский

Citations

29
Computational role of structure in neural activity and connectivity DOI
Srdjan Ostojic, Stefano Fusi

Trends in Cognitive Sciences, Journal Year: 2024, Volume and Issue: 28(7), P. 677 - 690

Published: March 28, 2024

Language: Английский

Citations

10
Separating cognitive and motor processes in the behaving mouse DOI
Munib A. Hasnain, Jaclyn E Birnbaum,

Juan Luis Ugarte Nunez

et al.

Nature Neuroscience, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

Language: Английский

Citations

1
Brain-wide presynaptic networks of functionally distinct cortical neurons DOI Creative Commons
Ana R. Inácio, Ka Chun Lam, Yuan Zhao

et al.

Nature, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 26, 2025

Abstract Revealing the connectivity of functionally identified individual neurons is necessary to understand how activity patterns emerge and support behaviour. Yet brain-wide presynaptic wiring rules that lay foundation for functional selectivity remain largely unexplored. Cortical neurons, even in primary sensory cortex, are heterogeneous their selectivity, not only stimuli but also multiple aspects Here, investigate underlying pyramidal behavioural state 1–10 somatosensory cortex (S1), we used two-photon calcium imaging, neuropharmacology, single-cell-based monosynaptic input tracing optogenetics. We show state-dependent stable over time. These minimally affected by direct neuromodulatory inputs driven primarily glutamatergic inputs. Analysis networks with distinct profiles revealed although state-related state-unrelated shared a similar pattern local within S1, long-range differed. Individual cortical irrespective properties, received converging from main S1-projecting areas. tracked smaller proportion motor larger thalamic Optogenetic suppression reduced this was externally driven. Our results reveal as substrate preconfigured network dynamics associated state.

Language: Английский

Citations

1
Progressive Thalamo‐Cortical Disconnection in Amyotrophic Lateral Sclerosis Genotypes: Structural Degeneration and Network Dysfunction of Thalamus‐Relayed Circuits DOI Creative Commons
Marlene Tahedl, Jana Kleinerová, Mark A. Doherty

et al.

European Journal of Neurology, Journal Year: 2025, Volume and Issue: 32(5)

Published: May 1, 2025

ABSTRACT Background The thalamus is a key subcortical hub of numerous corticobasal and corticocortical circuits mediating wealth cognitive, behavioural, sensory motor processes. While thalamic pathology increasingly recognised in amyotrophic lateral sclerosis, its degeneration often assessed isolation instead adopting network‐wise perspective assessing the integrity rich cortical projections. Methods A prospective imaging study was conducted cohort genetically stratified patients to assess structural functional thalamo‐cortical volumetric alterations longitudinally. Results white matter projections anterior cingulate cortex, cerebellum, dorsolateral prefrontal cortex (DLPFC), Heschl's gyrus, medial frontal gyrus (MFG), orbitofrontal parietal postcentral precentral (PreCG) affected at baseline ALS, which more marked C9orf72 hexanucleotide repeat carriers. Precentral cerebellar grey volumes are also reduced, particularly C9orf72. Longitudinal analyses capture progressive disconnection between regions (DLPFC MFG) both positive sporadic thalamo‐PreCG negative cohort. Functional connectivity revealed increasing thalamo‐cerebellar ALS thalamo‐DLPFC intermediate‐length CAG expansion carriers ATXN2 over time. Discussion Our data provide evidence extensive ALS. Corticobasal extrapyramidal, somatosensory, cognitive behavioural functions as disease progresses. support conceptualisation ‘network disease’ notion ‘what wires together degenerates together’.

Language: Английский

Citations

1
Superior colliculus bidirectionally modulates choice activity in frontal cortex DOI Creative Commons
Alyse M. Thomas, Weiguo Yang, Catherine Wang

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Nov. 14, 2023

Abstract Action selection occurs through competition between potential choice options. Neural correlates of are observed across frontal cortex and downstream superior colliculus (SC) during decision-making, yet how these regions interact to mediate remains unresolved. Here we report that SC can bidirectionally modulate drive activity in cortex. In the mouse, topographically matched formed a descending motor pathway for directional licking re-entrant loop via thalamus. During distinct neuronal populations both encoded opposing lick directions exhibited competitive interactions. GABAergic neurons ipsilateral locally inhibited glutamatergic contralateral choice. Activating or suppressing cell types could These results thus identify as major locus within broader action network.

Language: Английский

Citations

13
Top-down control of exogenous attentional selection is mediated by beta coherence in prefrontal cortex DOI Creative Commons
Agrita Dubey,

David A. Markowitz,

Bijan Pesaran

et al.

Neuron, Journal Year: 2023, Volume and Issue: 111(20), P. 3321 - 3334.e5

Published: July 26, 2023

Language: Английский

Citations

12
A Functional and Non-Homuncular Representation of the Larynx in the Primary Motor Cortex of Mice, a Vocal Non-Learner DOI Creative Commons
César D. M. Vargas,

Rajvi K. Agravat,

Elena N. Waidmann

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 6, 2024

Summary Vocalization is a complex behavior ranging from fully innate to advanced vocal learning. Vocal learning species possess primary motor cortex (M1) region that makes direct projections brainstem neurons, which are thought facilitate and fine modulation of vocalizations. Surprisingly, similar, albeit sparse, projection M1 was found in mice. Mice produce ultrasonic vocalizations (USV) appear be mostly innate. Modulation these USVs impacted by lesions this region, but genetic ablation the leads few, if any, changes USVs. It remained unclear whether has any control over organ non-learning species. In current study, we stimulation different parts mice can generate contractions laryngeal jaw muscles, with latencies suggestive both indirect neurons. Viral tracing reveals single- double-labeled populations cortical neurons simultaneously innervate laryngeal, jaw, forelimb circuits. Chemical reveal an anterolateral orofacial regulates number syllables sequences. Our results provide evidence non-learner some influence on musculature, consistent continuum hypothesis They also representations muscles for behaviors across mouse more intermixed than previously considered. We discuss how impact hypotheses evolution organization.

Language: Английский

Citations

4
Analysis methods for large-scale neuronal recordings DOI
Carsen Stringer, Marius Pachitariu

Science, Journal Year: 2024, Volume and Issue: 386(6722)

Published: Nov. 7, 2024

Simultaneous recordings from hundreds or thousands of neurons are becoming routine because innovations in instrumentation, molecular tools, and data processing software. Such can be analyzed with science methods, but it is not immediately clear what methods to use how adapt them for neuroscience applications. We review, categorize, illustrate diverse analysis neural population describe these have been used make progress on longstanding questions neuroscience. review a variety approaches, ranging the mathematically simple complex, exploratory hypothesis-driven, recently developed more established methods. also some common statistical pitfalls analyzing large-scale data.

Language: Английский

Citations

4
The Geometry and Dimensionality of Brain-wide Activity DOI Open Access
Zezhen Wang, Weihao Mai, Yuming Chai

et al.

Published: Jan. 8, 2025

Understanding neural activity organization is vital for deciphering brain function. By recording whole-brain calcium in larval zebrafish during hunting and spontaneous behaviors, we find that the shape of space, described by covariance spectrum, scale-invariant: a smaller, randomly sampled cell assembly resembles entire brain. This phenomenon can be explained Euclidean Random Matrix theory, where neurons are reorganized from anatomical to functional positions based on their correlations. Three factors contribute observed scale invariance: slow correlation decay, higher space dimension, heterogeneity. In addition matching data mice, our theory analysis demonstrate how geometry evolves with population sizes sampling methods, thus revealing an organizing principle brain-wide activity.

Language: Английский

Citations

0