Organization of corticocortical and thalamocortical top-down inputs in the primary visual cortex

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 27, 2024

Abstract Unified visual perception requires integration of bottom-up and top-down inputs in the primary cortex (V1), yet organization V1 remains unclear. Here, we used optogenetics-assisted circuit mapping to identify how multiple from higher-order cortical thalamic areas engage excitatory inhibitory neurons. Top-down overlap superficial layers segregate deep layers. Inputs medial secondary (V2M) anterior cingulate (ACA) converge on L6 Pyrs, whereas ventrolateral orbitofrontal (ORBvl) lateral posterior nucleus (LP) are processed parallel Pyr-type-specific subnetworks (Pyr ←ORBvl Pyr ←LP ) drive mutual inhibition between them via local interneurons. Our study deepens understanding modulation mechanisms processing establishes that V2M ACA employ integrated distinct LP ORBvl L5.

Language: Английский

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A transthalamic pathway crucial for perception

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 26, 2024

Perception is largely supported by cortical processing that involves communication among multiple areas, typically starting with primary sensory cortex and then involving higher order cortices. This served in part transthalamic (cortico-thalamo-cortical) pathways, which ubiquitously parallel direct corticocortical but their role has remained unexplored. Here, we suggest propagates task-relevant information required for correct decisions. Using optogenetics, specifically inhibited the pathway at its synapse somatosensory thalamus of mice performing a texture-based discrimination task. We concurrently monitored cellular effects inhibition or secondary using two-photon calcium imaging. Inhibition severely impaired performance despite intact projections, thus challenging purely corticocentric map perception. Interestingly, did not reduce overall cell responsiveness to texture stimulation cortex, rather disrupted selectivity cells, discriminability develops over task learning. was more than emphasizing feedforward influence route. Transthalamic pathways may therefore act deliver performance-relevant are underappreciated hierarchical perceptual decision-making.

Language: Английский

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Spatiotemporal dynamics in large-scale cortical networks

Current Opinion in Neurobiology, Journal Year: 2022, Volume and Issue: 77, P. 102627 - 102627

Published: Sept. 15, 2022

Language: Английский

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Dissecting cell‐type‐specific pathways in medial entorhinal cortical‐hippocampal network for episodic memory

Journal of Neurochemistry, Journal Year: 2023, Volume and Issue: 166(2), P. 172 - 188

Published: May 30, 2023

Abstract Episodic memory, which refers to our ability encode and recall past events, is essential daily lives. Previous research has established that both the entorhinal cortex (EC) hippocampus (HPC) play a crucial role in formation retrieval of episodic memories. However, understand neural circuit mechanisms behind these processes, it become necessary monitor manipulate activity cell‐type‐specific manner with high temporal precision during memory formation, consolidation, EC‐HPC networks. Recent studies using labeling, monitoring, manipulation have demonstrated medial EC (MEC) contains multiple excitatory neurons differential molecular markers, physiological properties, anatomical features. In this review, we will comprehensively examine complementary roles superficial layers (II III) deeper (V VI) based on recent findings. image

Language: Английский

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Wide-field imaging in behaving mice as a tool to study cognitive function

Neurophotonics, Journal Year: 2024, Volume and Issue: 11(03)

Published: Feb. 19, 2024

Cognitive functions are mediated through coordinated and dynamic neuronal responses that involve many different areas across the brain. Therefore, it is of high interest to simultaneously record activity from as brain possible while subject performs a cognitive behavioral task. One emerging tools achieve mesoscopic field view wide-field imaging cortex-wide dynamics in mice. Wide-field cost-effective, user-friendly, enables obtaining signals mice performing complex demanding tasks. Importantly, offers an unbiased observation sheds light on overlooked cortical regions highlights parallel processing circuits. Recent studies have shown multi-area patterns, rather than just single area, involved encoding functions. The optical properties enable signals, such layer-specific, inhibitory subtypes, or neuromodulation signals. Here, I review main advantages mice, recent literature, discuss future directions field. It expected behaving will continue gain popularity aid understanding mesoscale underlying function.

Language: Английский

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A transthalamic pathway crucial for perception

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: March 31, 2023

Perception arises from activity between cortical areas, first primary cortex and then higher order cortices. This communication is served in part by transthalamic (cortico-thalamo-cortical) pathways, which ubiquitously parallel direct corticocortical but their role sensory processing has largely remained unexplored. Here, we show that the pathway linking somatosensory cortices propagates task-relevant information required for correct decisions. Using optogenetics, specifically inhibited at its synapse thalamus of mice performing a texture-based discrimination task. We concurrently monitored cellular effects inhibition or secondary using two-photon calcium imaging. Inhibition severely impaired performance despite intact projections, thus challenging purely corticocentric map perception. Interestingly, did not reduce overall cell responsiveness to texture stimulation cortex, rather disrupted selectivity cells, discriminability develops over task learning. was more than emphasizing feedforward influence route. Transthalamic pathways appear critical delivering performance-relevant are hierarchical perceptual decision-making.

Language: Английский

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Linking brain activity across scales with simultaneous opto- and electrophysiology

Neurophotonics, Journal Year: 2023, Volume and Issue: 11(03)

Published: Sept. 1, 2023

The brain enables adaptive behavior via the dynamic coordination of diverse neuronal signals across spatial and temporal scales: from fast action potential patterns in microcircuits to slower distributed activity brain-wide networks. Understanding principles multiscale dynamics requires simultaneous monitoring multiple, network nodes. Combining optical electrical recordings is promising for collecting data multiple scales can reveal aspects coordinated invisible standard, single-modality approaches. We review recent progress combining opto- electrophysiology, focusing on mouse studies that shed new light function single neurons by embedding their context patterns. Optical readouts be tailored desired tackle specific questions. For example, cells or local populations recorded with multi-electrode arrays related simultaneously acquired report specified subpopulations neurons, non-neuronal cells, neuromodulatory pathways. Conversely, two-photon imaging used densely monitor circuits while sampling distant areas at same time. refinement combined approaches will continue previously inaccessible under-appreciated activity.

Language: Английский

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Cadherins orchestrate specific patterns of perisomatic inhibition onto distinct pyramidal cell populations

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 28, 2023

Abstract In the mammalian neocortex, excitatory pyramidal cells are assembled into distinct subnetworks, which project to different brain areas. GABAergic interneurons were long thought connect promiscuously and extensively neurons, but recent evidence supports existence of a cell type-specific inhibitory connectome. How when establish such precise connectivity pattern among intermingled populations neurons remains enigmatic. Here, we investigated molecular rules shaping type- input-specific in Layer 5 (L5) populations. We found that neighboring L5 intra- (L5 IT) extra-telencephalic ET) receive combinations perisomatic inputs from Parvalbumin- (PV+) Cholecystokynine-positive (CCK+) basket cells. also identified Cdh12 Cdh13 , two cadherin superfamily members, as critical mediators connectivity. Our data revealed minimal overlap between IT ET presynaptic networks, suggests PV+ innervate types. Altogether, our work unravels contribution cadherins cortical interneuron wiring provides new insights development microcircuits.

Language: Английский

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History information emerges in the cortex during learning

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Nov. 3, 2023

We learn from our experience but the underlying neuronal mechanisms incorporating past information to facilitate learning is relatively unknown. Specifically, which cortical areas encode history-related and how this modulated across learning? To study relationship between history learning, we continuously imaged cortex-wide calcium dynamics as mice use their whiskers discriminate two different textures. mainly focused on comparing same trial type with history, that is, a preceding trial. found in barrel cortex (BC) during stimulus presentation. Importantly, BC emerged only mouse learned task. Next, also learning-dependent rostrolateral (RL) association emerges before presentation, activity BC. Trial was encoded other not related differences body movements. Interestingly, binary classifier could at single level just well current both RL. These findings suggest around time of starting higher-order area RL propagating down (i.e., top-down projection) lower-order where it can be integrated incoming sensory information. This integration present may learning.

Language: Английский

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Wide-field calcium imaging of cortical activation and functional connectivity in externally- and internally-driven locomotion

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 6, 2024

Language: Английский

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