An applicable and efficient retrograde monosynaptic circuit mapping tool for larval zebrafish

Published: Aug. 20, 2024

The larval zebrafish is a vertebrate model for in vivo monitoring and manipulation of whole-brain neuronal activities. Tracing its neural circuits still remains challenging. Here we report an applicable methodology tailored to achieve efficient retrograde trans-monosynaptic tracing from genetically defined neurons via EnvA-pseudotyped glycoprotein-deleted rabies viruses. By combinatorially optimizing multiple factors involved, identified the CVS strain trans-complemented with advanced expression N2cG at 36°C as optimal combination. It yielded efficiency up 20 inputs per starter cell. Its low cytotoxicity enabled viable labeling calcium imaging infected 10 days post-infection, spanning ages commonly used functional examination. Cre-dependent was further developed enable input cell-type-specific circuit reconstruction. We mapped cerebellar uncovered ipsilateral preference subtype specificity granule cell-to-Purkinje cell connections. Our method offers way zebrafish.

Language: Английский

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Genetically defined nucleus incertus neurons differ in connectivity and function

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Aug. 31, 2023

The nucleus incertus (NI), a conserved hindbrain structure implicated in the stress response, arousal, and memory, is major site for production of neuropeptide relaxin-3. On basis goosecoid homeobox 2 ( gsc2 ) expression, we identified neuronal cluster that lies adjacent to relaxin 3a rln3a neurons zebrafish analogue NI. To delineate characteristics NI neurons, used CRISPR/Cas9 targeted integration drive gene expression specifically each group, found they differ their efferent afferent connectivity, spontaneous activity, functional properties. have widely divergent projection patterns innervate distinct subregions midbrain interpeduncular (IPN). Whereas are activated more robustly by electric shock, exhibit fluctuations calcium signaling regulate locomotor activity. Our findings define heterogeneous provide new tools probe its diverse functions.

Language: Английский

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Neuroscience: Fish and fly headed in the same direction

Current Biology, Journal Year: 2023, Volume and Issue: 33(12), P. R677 - R679

Published: June 1, 2023

Language: Английский

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Organization of vestibular circuits for postural control in zebrafish

Current Opinion in Neurobiology, Journal Year: 2023, Volume and Issue: 82, P. 102776 - 102776

Published: Aug. 25, 2023

Language: Английский

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How AI could lead to a better understanding of the brain

Nature, Journal Year: 2023, Volume and Issue: 623(7986), P. 247 - 250

Published: Nov. 7, 2023

Language: Английский

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