Pan-cortical 2-photon mesoscopic imaging and neurobehavioral alignment in awake, behaving mice DOI Creative Commons
Evan D. Vickers, David A. McCormick

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 23, 2023

The flow of neural activity across the neocortex during active sensory discrimination is constrained by task-specific cognitive demands, movements, and internal states. During behavior, brain appears to sample from a broad repertoire activation motifs. Understanding how these patterns local global are selected in relation both spontaneous task-dependent behavior requires in-depth study densely sampled at single neuron resolution large regions cortex. In significant advance toward this goal, we developed procedures record mesoscale 2-photon Ca

Language: Английский

Facemap: a framework for modeling neural activity based on orofacial tracking DOI Creative Commons
Atika Syeda, Lin Zhong, Renee Tung

et al.

Nature Neuroscience, Journal Year: 2023, Volume and Issue: 27(1), P. 187 - 195

Published: Nov. 20, 2023

Recent studies in mice have shown that orofacial behaviors drive a large fraction of neural activity across the brain. To understand nature and function these signals, we need better computational models to characterize relate them activity. Here developed Facemap, framework consisting keypoint tracker deep network encoder for predicting Our algorithm tracking mouse was more accurate than existing pose estimation tools, while processing speed several times faster, making it powerful tool real-time experimental interventions. The Facemap easy adapt data from new labs, requiring as few 10 annotated frames near-optimal performance. We used keypoints inputs which predicts ~50,000 simultaneously-recorded neurons and, visual cortex, doubled amount explained variance compared previous methods. Using this model, found neuronal clusters were well predicted behavior spatially spread out cortex. also behavioral features model had stereotypical, sequential dynamics not reversible time. In summary, provides stepping stone toward understanding brain-wide signals their relation behavior.

Language: Английский

Citations

56

Hippocampal neuronal activity is aligned with action plans DOI Creative Commons
Ipshita Zutshi, Athina Apostolelli, Wannan Yang

et al.

Nature, Journal Year: 2025, Volume and Issue: 639(8053), P. 153 - 161

Published: Jan. 8, 2025

Language: Английский

Citations

2

Toward a neuroscience of natural behavior DOI Creative Commons
Paul Cisek, Andrea M. Green

Current Opinion in Neurobiology, Journal Year: 2024, Volume and Issue: 86, P. 102859 - 102859

Published: April 6, 2024

One of the most exciting new developments in systems neuroscience is progress being made toward neurophysiological experiments that move beyond simplified laboratory settings and address richness natural behavior. This enabled by technological advances such as wireless recording freely moving animals, automated quantification behavior, methods for analyzing large data sets. Beyond empirical data, however, there also a need theories concepts to interpret data. Such particular challenges which often differ significantly from scenarios studied traditional settings. Here, we discuss some strategies developing novel example hypotheses proposed.

Language: Английский

Citations

15

Convergent vocal representations in parrot and human forebrain motor networks DOI
Zetian Yang, Michael A. Long

Nature, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

Language: Английский

Citations

1

Analysis methods for large-scale neuronal recordings DOI
Carsen Stringer, Marius Pachitariu

Science, Journal Year: 2024, Volume and Issue: 386(6722)

Published: Nov. 7, 2024

Simultaneous recordings from hundreds or thousands of neurons are becoming routine because innovations in instrumentation, molecular tools, and data processing software. Such can be analyzed with science methods, but it is not immediately clear what methods to use how adapt them for neuroscience applications. We review, categorize, illustrate diverse analysis neural population describe these have been used make progress on longstanding questions neuroscience. review a variety approaches, ranging the mathematically simple complex, exploratory hypothesis-driven, recently developed more established methods. also some common statistical pitfalls analyzing large-scale data.

Language: Английский

Citations

4

Experience reorganizes content-specific memory traces in macaques DOI Creative Commons
Saman Abbaspoor, Ayman Aljishi, Kari L. Hoffman

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

Abstract Memory formation requires neural activity reorganization during experience that persists in sleep. How these processes promote learning while preserving established memories remains unclear. We recorded ensemble from hippocampal and associated regions freely moving macaques as they recalled item sequences presented day (“new”), one prior (“recent”), or over two weeks (“old”). Cell assemblies biased for old showed less drift, greater network connectivity, stronger sleep reactivation than new-biased assemblies. Pairs of recent formed persistent task-to-sleep coupling (“metassemblies”), unlike new assembly pairs. In the hippocampus, propensity superficial deep CA1 pyramidal cells to form integrated increased with memory age. These findings reveal rapid organization stabilization primate brain, suggesting potential mechanisms balancing linking durability.

Language: Английский

Citations

0

Pan-cortical 2-photon mesoscopic imaging and neurobehavioral alignment in awake, behaving mice DOI Creative Commons
Evan D. Vickers, David A. McCormick

eLife, Journal Year: 2024, Volume and Issue: 13

Published: Jan. 23, 2024

The flow of neural activity across the neocortex during active sensory discrimination is constrained by task-specific cognitive demands, movements, and internal states. During behavior, brain appears to sample from a broad repertoire activation motifs. Understanding how these patterns local global are selected in relation both spontaneous task-dependent behavior requires in-depth study densely sampled at single neuron resolution large regions cortex. In significant advance toward this goal, we developed procedures record mesoscale 2-photon Ca2+ imaging data two novel vivo preparations that, between them, allow for simultaneous access nearly all 0f mouse dorsal lateral neocortex. As proof principle, aligned with behavioral primitives high-level motifs reveal existence populations neurons that coordinated their cortical areas changes movement and/or arousal. methods detail here facilitate identification exploration widespread, spatially heterogeneous ensembles whose related diverse aspects behavior.

Language: Английский

Citations

3

Reward Expectation Reduces Representational Drift in the Hippocampus DOI Creative Commons
Seetha Krishnan, Mark Sheffield

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 22, 2023

Spatial memory in the hippocampus involves dynamic neural patterns that change over days, termed representational drift. While drift may aid updating, excessive could impede retrieval. Memory retrieval is influenced by reward expectation during encoding, so we hypothesized diminished would exacerbate We found high limited drift, with CA1 representations on one day gradually re-emerging successive trials following day. Conversely, absence of resulted increased as gradual re-emergence previous day's representation did not occur. At single cell level, lowering caused an immediate increase proportion place-fields low trial-to-trial reliability. These place fields were less likely to be reinstated day, underlying this condition. In conclusion, heightened improves encoding and maintaining reliable are across thereby minimizing

Language: Английский

Citations

8

Pan-cortical 2-photon mesoscopic imaging and neurobehavioral alignment in awake, behaving mice DOI Creative Commons
Evan D. Vickers, David A. McCormick

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 23, 2023

The flow of neural activity across the neocortex during active sensory discrimination is constrained by task-specific cognitive demands, movements, and internal states. During behavior, brain appears to sample from a broad repertoire activation motifs. Understanding how these patterns local global are selected in relation both spontaneous task-dependent behavior requires in-depth study densely sampled at single neuron resolution large regions cortex. In significant advance toward this goal, we developed procedures record mesoscale 2-photon Ca

Language: Английский

Citations

1