Faraday Discussions, Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 23, 2024
Ion transport through biological channels is influenced not only by the structural properties of themselves but also composition phospholipid membrane, which acts as a scaffold for these nanochannels. Drawing inspiration from how lipid membrane modulates ion currents, seen in activation K
Language: Английский
JACS Au, Journal Year: 2025, Volume and Issue: 5(2), P. 486 - 520
Published: Feb. 10, 2025
Tetrahedral framework nucleic acids (tFNAs) represent a promising advancement in acid nanotechnology due to their unique structural properties, high biocompatibility, and multifaceted biomedical applications. Constructed through one-pot annealing method, four single-stranded DNAs self-assemble into stable, three-dimensional tetrahedral nanostructures with enhanced mechanical robustness physiological stability, resisting enzymatic degradation. Their ability permeate mammalian cells without transfection agents, coupled modifiable surfaces, positions tFNAs as versatile carriers for drug gene delivery systems. The tFNA-based platforms exhibit superior therapeutic efficacy, including antioxidative anti-inflammatory effects, alongside efficient cellular uptake tissue penetration. These features underpin role precision medicine, enabling targeted of diverse agents such synthetic compounds, peptides, acids. Additionally, demonstrate significant potential regenerative immune modulation, antibacterial strategies, oncology. By addressing challenges translational integration, stand poised accelerate the development research clinical applications, fostering novel therapies enhancing outcomes across wide spectrum diseases. This Perspective thoroughly details attributes applications critically evaluates tFNAs' potential, outlining inherent implementation exploring solutions these obstacles.
Language: Английский
Citations
2
Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 106614 - 106614
Published: Jan. 1, 2025
Language: Английский
Citations
1
Chem & Bio Engineering, Journal Year: 2025, Volume and Issue: 2(3), P. 171 - 181
Published: Jan. 31, 2025
Advancements in cell separation are essential for understanding cellular phenotypes and functions, with implications both research therapeutic applications. This review examines the evolution of techniques, categorizing them into physical affinity-based methods, a primary focus on latter due to its high specificity. Among affinity DNA nanomaterials have emerged as powerful tools biomolecular recognition owing their unique properties diverse range nanostructures. We discuss various nanomaterials, including linear aptamers, multivalent constructs, origami, hydrogels roles separation. Each section highlights distinctive characteristics these nanostructures, providing examples from recent studies that demonstrate applications isolation release. also compare four outlining individual contributions identifying remaining challenges opportunities further development. conclude nanotechnology holds great promise transformative solution separation, particularly context personalized therapeutics.
Language: Английский
Citations
0
Advanced Science, Journal Year: 2025, Volume and Issue: unknown
Published: April 7, 2025
Abstract Osteoarthritis (OA) is a chronic joint disease that causes gradual deterioration of articular cartilage. A major challenge in OA treatment the limited penetration and delivery efficiency drugs to cartilage chondrocytes due rapid clearance through synovial fluid joints osmotic barrier extracellular matrix (ECM). To address this issue, novel tetrahedral framework nucleic acid (tFNA)‐based nanomedicine system (tFNA‐2WL) first synthesized with excellent permeability perfect chondrocyte endocytosis properties. After being loaded ginsenoside Rb1 (Gin), tFNA‐2WL&Gin complex not only penetrates but also accumulates menisci, ligaments, capsules, thus prolonging residence time Gin rat knees. In vitro, effectively promotes chondrogenesis, inhibits degradation by reducing apoptosis, scavenges reactive oxygen species (ROS), outperforming free Gin. rats, restores gait, reduces osteophyte formation, inflammation hypertrophy, protects from further damage more than other nanomedicines. These results demonstrate feasibility tFNA‐2WL improving pharmacokinetics efficacy highlight favorable curative effects for OA, offering promising paradigm translational medicine.
Language: Английский
Citations
0
Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 3, 2025
Proteolysis-targeting chimeras (PROTACs) are dual-functional molecules composed of a protein interest (POI) ligand and an E3 ligase connected by linker, which can recruit POI ligases simultaneously, thereby inducing the degradation showing great potential in disease treatment. A challenge developing PROTACs is design linkers modification ligands to establish multifunctional platform that enhances efficiency antitumor activity. As programmable modifiable nanomaterial, DNA tetrahedron precisely assemble selectively recognize flexibly adjust distance between molecules, making them ideal linkers. Herein, we developed multivalent PROTAC based on tetrahedron, named AS-TD2-PRO. Using as combined modules targeting tumor cells, recognizing ligases, multiple together. We took undruggable target signal transducer activator transcription 3 (STAT3), associated with etiology progression variety malignant tumors, example this study. AS-TD2-PRO two STAT3 recognition demonstrated good enhancing tumor-specific compared traditional bivalent PROTACs. Furthermore, mouse model, superior therapeutic activity was observed. Overall, tetrahedron-driven both serve proof principle for introduce promising avenue cancer treatment strategies.
Language: Английский
Citations
0
Macromolecular Bioscience, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 21, 2025
Osteoporotic bone regeneration is challenging due to impaired formation. Tetrahedral DNA nanostructures (TDN), promising nucleic acid nanomaterials, have garnered attention for their potential in osteoporotic mandibular owing ability enhance cellular activity and promote osteogenic differentiation. Osteoblasts play a critical role regeneration; however, intracellular delivery of TDN into osteoblasts remains difficult. In this study, novel osteoblast-targeted CH6 aptamer-functionalized (TDN-CH6) aimed develop regeneration. This results demonstrated that TDN-CH6 exhibits superior osteoblast specificity efficient recruitment fracture sites. Furthermore, significantly enhances differentiation compared alone. Notably, Gelatin Methacryloyl (GelMA) hydrogels incorporating shows improved biological performance are favorable regeneration, suggesting platform represents strategy addressing complex defects.
Language: Английский
Citations
0
Small, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 9, 2025
Late-stage diagnosis is a major contributor to cancer mortality and thus leads increased fatality, making early detection crucial for improving survival rates. Circulating tumor cells (CTC), detectable before primary tumors become clinically apparent, have emerged as vital biomarkers the identification of aggressive cancers. Here, develop single-atom nanozyme integrated nanoarray 3D nano-biointerface ultrasensitive electrochemical screening CTCs from hepatocellular carcinoma. This cytosensor capable identifying CTC at single-cell level, achieving an impressive area under curve 0.96 in receiver operating characteristics, comparable simulated multi-indicator diagnostic strategies. strategy shows great potential non-invasive carcinoma promising be applied universally diagnosis.
Language: Английский
Citations
0
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: April 4, 2025
Circulating tumor cells (CTCs) have emerged as critical biomarkers in liquid biopsy for noninvasive diagnosis and real-time monitoring of cancer progression. However, the isolation CTCs is often required before detection due to their ultralow abundance peripheral blood. These processes are typically time-consuming prone cell loss, which limits utility CTC-based biopsy. Herein, we present a DNA network-based diagnostic system that enables specific recognition, selective enrichment, accurate directly from blood samples. The network comprises ultralong chains embedded with polyvalent aptamers fluorescence modules. selectively bind epithelial adhesion molecule (EpCAM) on CTC membrane, facilitating enrichment through base pairing-driven formation. This semiquantitatively detects expression level cancer-associated microRNA within using ratiometric imaging based chemical assembly two In clinical samples, this achieves 100% precision 96% accuracy distinguishing breast patients healthy donors, highlighting its promising potential diagnosis.
Language: Английский
Citations
0
Acta Pharmaceutica Sinica B, Journal Year: 2025, Volume and Issue: unknown
Published: April 1, 2025
Language: Английский
Citations
0
Science Advances, Journal Year: 2024, Volume and Issue: 10(35)
Published: Aug. 30, 2024
The transport of ions through biological ion channels is regulated not only by their structural characteristics but also the composition phospholipid membrane, which serves as a carrier for nanochannels. Inspired modulation currents lipid membrane composition, exemplified activation K
Language: Английский
Citations
2