Microglia activation visualization via fluorescence lifetime imaging microscopy of intrinsically fluorescent metabolic cofactors DOI Creative Commons
Md Abdul Kader Sagar,

Jonathan N. Ouellette,

Kevin Cheng

et al.

Neurophotonics, Journal Year: 2020, Volume and Issue: 7(03), P. 1 - 1

Published: Aug. 8, 2020

Significance: A major obstacle to studying resident microglia has been their similarity infiltrating immune cell types and the lack of unique protein markers for identifying functional state. Given role in all neural diseases insults, accurate tools detecting function beyond morphologic alterations are necessary. Aims: We hypothesized that would have metabolic fluxes reduced nicotinamide adenine dinucleotide (NADH) be detectable by relative changes fluorescence lifetime imaging microscopy (FLIM) parameters, allowing identification activation status. Fluorescence NADH previously demonstrated show differences fluxes. Approach: Here, we investigate use label-free method FLIM-based detection endogenous cofactor identify characterize To test whether microglial also confer a signature, murine primary cultures adult mice were treated with lipopolysaccharide (LPS). Results: found LPS-induced correlates detected its free-bound ratio. This indicates can used monitor fashion. Moreover, there is an LPS dose-dependent change associated reactive fluxes, which replicated over time after treatment. Conclusion: way monitoring via quantifying coenzyme NADH. Upon activation, significant following activation. Together, these results indicate FLIM approaches as state, both vitro ex vivo.

Language: Английский

Understanding Real-Time Fluorescence Signals from Bacteria and Wound Tissues Observed with the MolecuLight i:XTM DOI Creative Commons
Monique Y. Rennie,

Danielle Dunham,

Liis Lindvere‐Teene

et al.

Diagnostics, Journal Year: 2019, Volume and Issue: 9(1), P. 22 - 22

Published: Feb. 26, 2019

The persistent presence of pathogenic bacteria is one the main obstacles to wound healing. Detection relies on sampling methods, which delay confirmation by several days. However, a novel handheld fluorescence imaging device has recently enabled real-time detection in wounds based their intrinsic characteristics, differ from those background tissues. This illuminates with violet (405 nm) light, causing tissues and produce endogenous, characteristic signals that are filtered displayed screen real-time. resulting images allow for rapid assessment documentation presence, location, extent fluorescent at moderate-to-heavy loads. information been shown assist guide patient-specific treatment plans. proper image interpretation essential assessing this information. To properly identify regions bacterial fluorescence, users must understand: (1) Fluorescence (e.g., tissues, tendon, bone) fluids blood, pus); (2) (red or cyan); (3) rationale varying hues both tissue fluorescence; (4) artifacts can occur; (5) some potentially confounding non-biological materials cleansing solutions). Therefore, tutorial provides clinicians identifying common characteristics. Clinical examples intended help interpretation-with focus potential confounders interpretation-and suggestions how overcome such challenges when clinical practice.

Language: Английский

Citations

91
Metabolic heterogeneity in clonal microbial populations DOI Creative Commons
Vakil Takhaveev, Matthias Heinemann

Current Opinion in Microbiology, Journal Year: 2018, Volume and Issue: 45, P. 30 - 38

Published: Feb. 22, 2018

In the past decades, numerous instances of phenotypic diversity were observed in clonal microbial populations, particularly, on gene expression level. Much less is, however, known about differences that occur level metabolism. This is likely explained by fact experimental tools probing metabolism single cells are still at an early stage development. Here, we review recent exciting discoveries point out different causes for metabolic heterogeneity within populations. These range from ecological factors and cell-inherent dynamics constant environments to molecular noise propagates into Furthermore, provide overview current methods quantify levels metabolites biomass components cells.

Language: Английский

Citations

90
Roadmap on emerging concepts in the physical biology of bacterial biofilms: from surface sensing to community formation DOI Creative Commons
Gerard C. L. Wong, Jyot D. Antani, Pushkar P. Lele

et al.

Physical Biology, Journal Year: 2021, Volume and Issue: 18(5), P. 051501 - 051501

Published: Jan. 19, 2021

Bacterial biofilms are communities of bacteria that exist as aggregates can adhere to surfaces or be free-standing. This complex, social mode cellular organization is fundamental the physiology microbes and often exhibits surprising behavior. more than sum their parts: single-cell behavior has a complex relation collective community behavior, in manner perhaps cognate between atomic physics condensed matter physics. Biofilm microbiology relatively young field by biology standards, but it already attracted intense attention from physicists. Sometimes, this takes form seeing inspiration for new In roadmap, we highlight work those who have taken opposite strategy: physicists physical scientists use engage concepts bacterial biofilm microbiology, including adhesion, sensing, motility, signaling, memory, energy flow, formation cooperativity. These contributions juxtaposed with microbiologists made recent important discoveries on using state-of-the-art methods. The roadmap exemplify how well combined achieve synthesis, rather just division labor.

Language: Английский

Citations

72
Chemical composition, antibacterial activity and study of the interaction mechanisms of the main compounds present in the Alpinia galanga rhizomes essential oil DOI

Changqian Zhou,

Changzhu Li, Subramanian Siva

et al.

Industrial Crops and Products, Journal Year: 2021, Volume and Issue: 165, P. 113441 - 113441

Published: March 21, 2021

Language: Английский

Citations

68
Rapid species identification of pathogenic bacteria from a minute quantity exploiting three-dimensional quantitative phase imaging and artificial neural network DOI Creative Commons
Geon Kim,

Daewoong Ahn,

Minhee Kang

et al.

Light Science & Applications, Journal Year: 2022, Volume and Issue: 11(1)

Published: June 23, 2022

The healthcare industry is in dire need of rapid microbial identification techniques for treating infections. Microbial infections are a major issue worldwide, as these widespread diseases often develop into deadly symptoms. While studies have shown that an early appropriate antibiotic treatment significantly reduces the mortality infection, this effective difficult to practice. main obstacle treatments long turnaround time routine identification, which includes time-consuming sample growth. Here, we propose microscopy-based framework identifies pathogen from single few cells. Our obtains and exploits morphology limited by incorporating three-dimensional quantitative phase imaging artificial neural network. We demonstrate 19 bacterial species cause bloodstream infections, achieving accuracy 82.5% individual cell or cluster. This performance, comparable gold standard mass spectroscopy under sufficient amount sample, underpins effectiveness our clinical applications. Furthermore, increases with multiple measurements, reaching 99.9% seven different measurements cells clusters. believe can serve beneficial advisory tool clinicians during initial

Language: Английский

Citations

43
Environmental drivers of metabolic heterogeneity in clonal microbial populations DOI
Frank Schreiber, Martin Ackermann

Current Opinion in Biotechnology, Journal Year: 2019, Volume and Issue: 62, P. 202 - 211

Published: Dec. 23, 2019

Language: Английский

Citations

55
Probing organoid metabolism using fluorescence lifetime imaging microscopy (FLIM): The next frontier of drug discovery and disease understanding DOI Creative Commons
Margarida Barroso, Michael G. Monaghan, Raluca Niesner

et al.

Advanced Drug Delivery Reviews, Journal Year: 2023, Volume and Issue: 201, P. 115081 - 115081

Published: Aug. 28, 2023

Language: Английский

Citations

18
A Shift in Central Metabolism Accompanies Virulence Activation in Pseudomonas aeruginosa DOI Creative Commons
Kumar Perinbam, Jenu V. Chacko, Anerudh Kannan

et al.

mBio, Journal Year: 2020, Volume and Issue: 11(2)

Published: March 9, 2020

The rise of antibiotic resistance requires the development new strategies to combat bacterial infection and pathogenesis. A major direction has been drugs that broadly target virulence. However, few targets have identified due species-specific nature many virulence regulators. lack a regulator is conserved across species presented further challenge therapeutics. Here, we identify NADH activity an important role in induction pathogen P. aeruginosa . This finding, coupled with ubiquity pathogens, opens up possibility targeting enzymes process as potential broad antivirulence approach.

Language: Английский

Citations

43
Optical methods for bacterial detection and characterization DOI Creative Commons
Cushla McGoverin, Craig Steed, Ayomikun Esan

et al.

APL Photonics, Journal Year: 2021, Volume and Issue: 6(8)

Published: Aug. 1, 2021

Rapid identification and enumeration of bacteria are critical, given the surge antibiotic-resistance, global exchange food products, use for bioremediation, pharmaceutical, production. In response, a wide range methods being developed that can be broadly classified as nucleic acid-based, structure-based, mass spectrometry-based, optically based. Optical have generated interest potential rapid, non-destructive, high-throughput, amplification-free measurements require minimal sample preparation. This Perspective reviews optical methods, which applied to identification, enumeration, greater understanding routinely more importantly at cutting edge research, with aim identifying gaps opportunities development. We focused primarily on directly measure not their effect matrix or sensing, requires biorecognition element (i.e., label specific some component bacterium). identify in existing techniques avenues innovation. Finally, we suggest parameters should considered recorded when reporting development new bacterial characterization. is intended physicists interested developing study microbiologists need an technique applications.

Language: Английский

Citations

35
Restructured Mitochondrial-Nuclear Interaction in Plasmodium falciparum Dormancy and Persister Survival after Artemisinin Exposure DOI Creative Commons
Sean V. Connelly,

Javier Manzella‐Lapeira,

Zoë C. Levine

et al.

mBio, Journal Year: 2021, Volume and Issue: 12(3)

Published: May 24, 2021

Artemisinin and its semisynthetic derivatives (ART) are fast acting, potent antimalarials; however, their use in malaria treatment is frequently confounded by recrudescences from bloodstream Plasmodium parasites that enter into later reactivate a dormant persister state. Here, we provide evidence the mitochondria of dihydroartemisinin (DHA)-exposed persisters dramatically altered enlarged relative to young, actively replicating ring forms. Restructured mitochondrial-nuclear associations an metabolic state consistent with stress reactive oxygen species. New contacts between nuclei may support communication pathways mitochondrial retrograde signaling, resulting transcriptional changes nucleus as survival response. Further characterization organelle dependencies suggest strategies combat after treatment. IMPORTANCE The major first-line for malaria, especially deadliest form caused falciparum, combination therapy artemisinin-based drug plus partner assure complete cure. Without effective drug, ART administration alone can fail because ability small populations blood-stage survive repeated treatments week or more. Understanding nature dormancy (persisters) wake reestablish propagating parasitemias (recrudesce) exposure improve outcomes counter threats posed develop resistance drugs. show have interactions associated features quiescence. signaling enable responses dormancy.

Language: Английский

Citations

34