The Microbe, Journal Year: 2024, Volume and Issue: 6, P. 100226 - 100226
Published: Dec. 11, 2024
Language: Английский
Deleted Journal, Journal Year: 2025, Volume and Issue: 77(3)
Published: April 17, 2025
Language: Английский
Citations
0
Microbiology Research, Journal Year: 2025, Volume and Issue: 16(4), P. 86 - 86
Published: April 19, 2025
The Clonostachys genus is a saprophytic soil microfungus (Ascomycota). It exhibits significant ecological adaptability and plays crucial role in maintaining the balance of microorganisms. Species within this are natural antagonists insects nematodes, they also combat phytopathogenic fungi through mycoparasitism. This process involves producing lytic enzymes competing for space nutrients. species effective biocontrol agents agriculture have been utilized to manage pests affecting many high-value commercial crops, acting as biopesticide. They inhabit plant tissues, boosting defenses activating genes water nutrient uptake, enhancing performance. Additionally, produce bioactive metabolites with antimicrobial, antifungal, nematocidal, anticancer, antioxidant properties. can degrade plastic waste remove hydrocarbons from crude oil-contaminated sites when functioning endophytes, positioning promising candidate reducing environmental pollution. There still challenges limitations, such continuous surveillance safety on plants, establishment applications, formulation viability, variability due field conditions. These issues will be addressed. review provides an overview ecology, morphology, classification, biotechnological emphasizing its significance various fields.
Language: Английский
Citations
0
BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)
Published: May 2, 2025
Abstract Background Beneficial microorganisms can act as biological control agents (BCAs) directly by targeting pathogens or indirectly enhancing the plant’s defense mechanisms against pathogens. However, efficiencies with which plants benefit from BCAs vary, potentially because of genetic variation in for plant-BCA compatibility. The aim this study was to explore winter wheat modulation Clonostachys rosea -mediated biocontrol septoria tritici blotch disease caused fungal pathogen Zymoseptoria . Results In total, 202 genotypes, including landraces and old cultivars grown 1900 onwards Scandinavian countries, were tested under greenhouse-controlled conditions. Foliar spray applications BCA two treatments, i.e., Z. (Zt) alone along C. (ZtCr) used assess progress over time. absence presence Zt ZtCr, respectively, allowed dissection plant resistance efficacy. showed significant ( P < 0.05) phenotypic among genotypes progression both ZtCr treatments. Moreover, application resulted a reduction seven increased eleven indicating genotype-dependent effect on interaction between wheat, For efficacy, genome-wide association using 20K single-nucleotide polymorphism (SNP) marker array also performed. five distinct SNP markers associated four efficacy identified. Conclusions This work serves foundation further characterize basis interactions when inoculated , facilitating opportunities simultaneous breeding
Language: Английский
Citations
0
Frontiers in Microbiology, Journal Year: 2018, Volume and Issue: 9
Published: June 7, 2018
strain ACM941 is a fungal bio-control agent patented against the causative of Fusarium Head Blight,
Language: Английский
Citations
32
Phytopathology, Journal Year: 2019, Volume and Issue: 109(10), P. 1698 - 1709
Published: May 23, 2019
Secondary metabolites produced by biological control agents may influence the outcome of their interactions with plant pathogenic microorganisms and plants. In present study, we investigated role nonribosomal peptide synthetase gene nps1 expressed biocontrol fungus Clonostachys rosea. A expression analysis showed that was induced during confrontations Botrytis cinerea. Gene deletion strains displayed increased growth rates conidiation. However, nematicidal activity culture filtrates from C. rosea Δnps1 significantly weaker than wild-type (P ≤ 0.001); after 24 h incubation strains, only 13 to 33% a mixed community nematodes were dead compared 42% incubated filtrates. The also reduced efficacy pot experiments, thus failing protect wheat seedlings foot rot disease caused Fusarium graminearum. Furthermore, not able reduce populations plant-parasitic in soil or roots as efficiently strain. Both dry shoot weight length 20 13%, respectively. We NPS1, putative encoded nps1, is factor, presumably producing hitherto unknown compound antifungal properties contributes
Language: Английский
Citations
28
Evolutionary Applications, Journal Year: 2020, Volume and Issue: 14(2), P. 476 - 497
Published: Sept. 17, 2020
Various strains of the mycoparasitic fungal species Clonostachys rosea are used commercially as biological control agents for plant diseases in agricultural crop production. Further improvements use and efficacy C. biocontrol require a mechanistic understanding factors that determines outcome interaction between pathogenic fungi. Here, we determined genome sequences 11 strains, representing five subgenus Bionectria, performed comparative genomic analysis with aim to identify gene families evolving under selection gains or losses. Several predicted encode proteins involved biosynthesis secondary metabolites, including polyketide synthases, nonribosomal peptide syntethases cytochrome P450s, evolved (p ≤ .05) Bionectria lineage. This was accompanied copy number increases ATP-binding cassette (ABC) transporters major facilitator superfamily (MFS) contribute drug efflux. Most were also characterized by high numbers auxiliary activity (AA) family 9 lytic polysaccharide monooxygenases, AA3 glucose-methanol-choline oxidoreductases additional carbohydrate-active enzyme putative (or binding) towards xylan rhamnose/pectin substrates. Particular features included expansions ABC-B4 multidrug resistance transporters, ABC-C5 resistance-related 2.A.1.3 drug:H + antiporter-2 MFS transporters. The ABC-G1 pleiotropic transporter abcG6 induced .009) exposure antifungal Fusarium mycotoxin zearalenone (1121-fold) various fungicides. Deletion resulted mutants reduced < .001) growth rates on media containing fungicides boscalid, fenhexamid iprodione. Our results emphasize role of, protection against, metabolites Bionectria.
Language: Английский
Citations
27
Pathogens, Journal Year: 2021, Volume and Issue: 10(6), P. 701 - 701
Published: June 4, 2021
Aspergillus fumigatus is a ubiquitous fungus and the main agent of aspergillosis, common fungal infection in immunocompromised population. Triazoles such as itraconazole voriconazole are first-line drugs for treating aspergillosis. However, triazole resistance A. has been reported an increasing number countries. While most studies have focused on mutations target gene cyp51A, >70% triazole-resistant strains certain populations showed no cyp51A. To identify potential non-cyp51A associated with fumigatus, we analyzed whole genome sequences susceptibilities 195 from 12 These belonged to three distinct clades. Our genome-wide association study (GWAS) identified total six missense significantly 18 resistance. In addition, investigate pan-azole resistance, Fisher's exact tests revealed 26 additional variants tightly linked top 20 SNPs obtained by GWAS, which two were consistently The large novel related should help further investigations into their molecular mechanisms, clinical importance, development comprehensive diagnosis toolbox fumigatus.
Language: Английский
Citations
21
PLoS ONE, Journal Year: 2022, Volume and Issue: 17(9), P. e0273985 - e0273985
Published: Sept. 6, 2022
The present study aimed to examine the capability of Clonostachys rosea isolates as a biological control agent against grapevine trunk diseases pathogens. Five C . and 174 pathogenic fungal strains were isolated from grafted grapevines subjected in vitro confrontation tests. Efficient antagonism was observed Eutypa lata Phaeomoniella chlamydospora while mycoparasitism pathogens Botryosphaeria dothidea Diaporthe spp. dual culture assays. conidia production also measured on PDA plates. One isolate (19B/1) with high antagonistic capabilities efficient selected for planta tests by mixing its soil Cabernet sauvignon cuttings artificially infected B , E P length and/or incidence necrotic lesions caused at inoculation point significantly decreased after three months incubation greenhouse planted soils inoculated strain 19B/1, symptom severity unaffected case pathogen Based above results, we consider promising some diseases.
Language: Английский
Citations
16
European Journal of Plant Pathology, Journal Year: 2023, Volume and Issue: 167(4), P. 453 - 476
Published: Sept. 8, 2023
Abstract Many Fusarium species cause serious diseases for cereal cultivation. These include head blight and crown rot on wheat bakanae disease rice. represent a major concern both in terms of food security safety. The latter is connected with the risk mycotoxin contamination grains. Biological control has proven its potential controlling cereals caused by number studies, indeed several commercial products are under development. We review current knowledge mechanisms underlying biological focus fungal biocontrol agents, also challenges related to co-occurrence species. Several established (antibiosis, competition, hyperparasitism induced resistance) can act simultaneously, thus resulting and, consequently, reduction contamination. roles some many mycotoxins produced species, which they detoxified enzymes or other fungi how agents (BCAs) stimulate their degradation. Finally, effect resident microbiota, as well microbiota performances BCAs, discussed. New perspectives use management cereals.
Language: Английский
Citations
9
Phytopathology, Journal Year: 2021, Volume and Issue: 111(10), P. 1697 - 1710
Published: April 28, 2021
Heavy reliance on synthetic pesticides for crop protection has become increasingly unsustainable, calling robust alternative strategies that do not degrade the environment and vital ecosystem services. There are numerous reports of successful disease control by various microbes used in small-scale trials. However, inconsistent efficacy hampered their large-scale application. A better understanding how beneficial interact with plants, other microbes, which factors affect is crucial to deploy microbial agents as effective reliable pesticide alternatives. Diverse metabolites produced plants participate pathogenesis defense, regulate growth development themselves neighboring organisms, help maintain cellular homeostasis under environmental conditions, assembly activity plant soil microbiomes. research associated health-related processes, except antibiotics, received adequate attention. This review highlights several classes known or suspected health, focusing those biocontrol belowground plant–microbe microbe–microbe interactions. The also describes new insights from systematic explorations diversity mechanism action bioactive can be harnessed develop novel strategies.
Language: Английский
Citations
18