Advances and Perspectives in the Use of Biocontrol Agents against Fungal Plant Diseases

Horticulturae, Journal Year: 2022, Volume and Issue: 8(7), P. 577 - 577

Published: June 25, 2022

The use of synthetic fungicides to control fungal diseases has growing limitations due eco-toxicological risks. Therefore, it is necessary replace or integrate high risk chemicals with safer tools for human health and environment. Consequently, research on the selection, evaluation, characterization, biocontrol agents (BCAs) consistently increased in last decades. BCA formulates, particularly some countries, are still scarce coping demand their sustainable agricultural management. To foster development utilization new effective bioformulates, there a need optimize activity, share knowledge formulation processes simplify registration procedures. Studies based molecular can significantly contribute achieve such objectives. present review provides state art plant special emphasis (i) features most studied BCAs; (ii) key strategies selection BCAs (iii); mechanisms action main (iv) metagenomic studies (v) issues constraints commercialization BCAs, (vi) perspectives diseases.

Language: Английский

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Biology and applications of Clonostachys rosea

Journal of Applied Microbiology, Journal Year: 2020, Volume and Issue: 129(3), P. 486 - 495

Published: March 2, 2020

Clonostachys rosea is a promising saprophytic filamentous fungus that belongs to phylum Ascomycota. widespread around the world and exists in many kinds of habitats, with highest frequency soil. As an excellent mycoparasite, C. exhibits strong biological control ability against numerous fungal plant pathogens, nematodes insects. These behaviours are based on activation multiple mechanisms such as secreted cell-wall-degrading enzymes, production antifungal secondary metabolites induction defence systems. Besides having significant biocontrol activity, also functions biodegradation plastic waste, biotransformation bioactive compounds, bioenergy sources fermentation. This mini review summarizes information about biology various applications expands its possible uses.

Language: Английский

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Biological control of strawberry crown rot, root rot and grey mould by the beneficial fungus Aureobasidium pullulans

BioControl, Journal Year: 2021, Volume and Issue: 66(4), P. 535 - 545

Published: March 18, 2021

Abstract Utilization of biocontrol agents is a sustainable approach to reduce plant diseases caused by fungal pathogens. In the present study, we tested effect candidate fungus Aureobasidium pullulans (De Bary) G. Armaud on strawberry under in vitro and vivo conditions control crown rot, root rot grey mould Phytophthora cactorum (Lebert Cohn) Botrytis cinerea Pers, respectively. A dual plate confrontation assay showed that mycelial growth P. B. was reduced 33–48% when challenged A. as compared with treatments. Likewise, detached leaf fruit assays significantly necrotic lesion size leaves disease severity fruits . addition, greenhouse experiments whole plants revealed enhanced efficacy against treated either combination pathogen or pre-treated followed inoculation Our results demonstrate an effective agent pathogens can be alternative chemical-based fungicides.

Language: Английский

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An anecdote of mechanics for Fusarium biocontrol by plant growth promoting microbes

Biological Control, Journal Year: 2022, Volume and Issue: 174, P. 105012 - 105012

Published: Aug. 6, 2022

Language: Английский

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Transcriptomic and Exometabolomic Profiling Reveals Antagonistic and Defensive Modes ofClonostachys roseaAction AgainstFusarium graminearum

Molecular Plant-Microbe Interactions, Journal Year: 2020, Volume and Issue: 33(6), P. 842 - 858

Published: March 2, 2020

The mycoparasite Clonostachys rosea ACM941 is under development as a biocontrol organism against Fusarium graminearum, the causative agent of head blight in cereals. To identify molecular factors associated with this interaction, transcriptomic and exometabolomic profiles C. F. graminearum GZ3639 were compared during coculture. Prior to physical contact, antagonistic activity correlated response heavily dominated by upregulation polyketide synthase gene clusters, consistent detected accumulation corresponding secondary metabolite products. Similarly, prior trichothecene clusters upregulated while those responsible for fusarielin fusarin biosynthesis downregulated, correlating an products interaction zone over time. A concomitant increase 15-acetyl deoxynivalenol-3-glucoside was also detected, established source detoxified mycotoxin. After hyphal found predominantly transcribe genes encoding cell wall–degradation enzymes, major facilitator superfamily sugar transporters, anion:cation symporters, well alternative carbon utilization pathways, together indicative transition necrotropism at stage. notably activated transcription phosphate starvation pathway signature Overall, number mechanisms likely contributing its mycotoxin tolerance, are identified report, yielding several new testable hypotheses toward understanding basis continued agronomic application.

Language: Английский

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Bee-Vectored Aureobasidium pullulans for Biological Control of Gray Mold in Strawberry

Phytopathology, Journal Year: 2021, Volume and Issue: 112(2), P. 232 - 237

Published: June 28, 2021

Gray mold caused by Botrytis cinerea is a common postharvest disease in strawberries, reducing shelf life considerably. We investigated the potential of yeast-like biocontrol fungus Aureobasidium pullulans (AP-SLU6) vectored bumblebees (Bombus terrestris) Flying Doctors® system to inhibit pathogen and increase harvested strawberries (cultivar Sonata). Using as vectors various agents becoming increasingly popular, but any potentially negative effects on bee performance have been understudied. Our results show that, over 4-week period trial, activity bees were not negatively affected A. pullulans. The successfully picked up powder formulation; then, they carried deposited it flowers. vectoring agent significantly reduced gray development fruits 45% increased 100% comparison with control treatments. This suggests that applied during flowering infection thus, effectively protected from mold. In addition, bee-vectored application was found be more effective than spray because latter may temporarily humidity around flower, thereby creating suitable environment for thrive. summary, our study demonstrates can decrease improve without adversely affecting bees, thus providing basis sustainable efficient strawberry.[Formula: see text] Copyright © 2022 Author(s). an open access article distributed under CC BY 4.0 International license.

Language: Английский

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Clonostachys Genus as a Source of Metabolites for Agricultural Applications

Fungal biology, Journal Year: 2025, Volume and Issue: unknown, P. 233 - 248

Published: Jan. 1, 2025

Language: Английский

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Comparative genomics highlights the importance of drug efflux transporters during evolution of mycoparasitism in Clonostachys subgenus Bionectria (Fungi, Ascomycota, Hypocreales)

Evolutionary Applications, Journal Year: 2020, Volume and Issue: 14(2), P. 476 - 497

Published: Sept. 17, 2020

Various strains of the mycoparasitic fungal species Clonostachys rosea are used commercially as biological control agents for plant diseases in agricultural crop production. Further improvements use and efficacy C. biocontrol require a mechanistic understanding factors that determines outcome interaction between pathogenic fungi. Here, we determined genome sequences 11 strains, representing five subgenus Bionectria, performed comparative genomic analysis with aim to identify gene families evolving under selection gains or losses. Several predicted encode proteins involved biosynthesis secondary metabolites, including polyketide synthases, nonribosomal peptide syntethases cytochrome P450s, evolved (p ≤ .05) Bionectria lineage. This was accompanied copy number increases ATP-binding cassette (ABC) transporters major facilitator superfamily (MFS) contribute drug efflux. Most were also characterized by high numbers auxiliary activity (AA) family 9 lytic polysaccharide monooxygenases, AA3 glucose-methanol-choline oxidoreductases additional carbohydrate-active enzyme putative (or binding) towards xylan rhamnose/pectin substrates. Particular features included expansions ABC-B4 multidrug resistance transporters, ABC-C5 resistance-related 2.A.1.3 drug:H + antiporter-2 MFS transporters. The ABC-G1 pleiotropic transporter abcG6 induced .009) exposure antifungal Fusarium mycotoxin zearalenone (1121-fold) various fungicides. Deletion resulted mutants reduced < .001) growth rates on media containing fungicides boscalid, fenhexamid iprodione. Our results emphasize role of, protection against, metabolites Bionectria.

Language: Английский

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Biochemical and Biotechnological Insights into Fungus-Plant Interactions for Enhanced Sustainable Agricultural and Industrial Processes

Plants, Journal Year: 2023, Volume and Issue: 12(14), P. 2688 - 2688

Published: July 19, 2023

The literature is full of studies reporting environmental and health issues related to using traditional pesticides in food production storage. Fortunately, alternatives have arisen the last few decades, showing that organic agriculture possible economically feasible. And this scenario, fungi may be helpful. In natural environment, when associated with plants, these microorganisms offer plant-growth-promoting molecules, facilitate plant nutrient uptake, antagonize phytopathogens. It true can also phytopathogenic, but even they benefit some way—since pathogenicity species-specific, are shown useful against weeds (as bioherbicides). Finally, plant-associated yeasts molds biofactories, metabolites produce while dwelling leaves, flowers, roots, or rhizosphere potential employed different industrial activities. By addressing all subjects, manuscript comprehensively reviews biotechnological uses and, addition, aims sensitize academics, researchers, investors new for healthier more environmentally friendly processes.

Language: Английский

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Unveiling the antimicrobial and biocontrol potential of the Ascomycete fungus, Clonostachys rosea: A review

The Microbe, Journal Year: 2024, Volume and Issue: 6, P. 100226 - 100226

Published: Dec. 11, 2024

Language: Английский

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