The Transcriptome Profiling of Flavonoids and Bibenzyls Reveals Medicinal Importance of Rare Orchid Arundina graminifolia DOI Creative Commons
Sagheer Ahmad, Jie Gao, Yonglu Wei

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: June 23, 2022

Orchids are very important flowering plants that spend long juvenile phases before flowering. Along with aesthetic importance, they rich sources of medicinal components. However, their reproductive cycle is the major hurdle to study efficacy. Arundina graminifolia a rare orchid grows fast, unlike other orchids, and this characteristic makes it an ideal plant enrichment orchids. Therefore, presents identification components in various parts A. . Transcriptome analysis was performed for five stages (FD1–FD5) flower development four tissue types (mature flower, silique, root, leaf) ascertain genetic regulators flavonoids bibenzyls. Most genes showed highest expression roots as compared tissues. Weighted gene coexpression network (WGCNA) identify modules candidate involving biosynthesis pathways these chemicals. MEyellow module contained highly coexpressed genes. Moreover, concentrations phenylpropanoid, bibenzyls, flavone were ascertained through high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Phenylpropanoid bibenzyl comparatively high leaf, while concentration stem. The selected [bibenzyl (BIBSY212), CYP84A1, CYP73A4, 4CLL7, UGT88B1, UGT73C3, anthocyanin synthase (ANS), phenylalanine ammonia-lyase (PAL), flavanone FLS, CHS8] validated quantitative real-time PCR (qRT-PCR). leaf root tissue. presence bibenzyls different molecular can provide quick source decipher efficacy

Language: Английский

Growth and alkaloid production along with expression profiles of biosynthetic pathway genes in two contrasting morphotypes of prickly and prickleless Solanum viarum Dunal DOI
Archana Prasad, Preeti Patel, Shatrujeet Pandey

et al.

PROTOPLASMA, Journal Year: 2019, Volume and Issue: 257(2), P. 561 - 572

Published: Dec. 9, 2019

Language: Английский

Citations

16
In vitro propagation, genetic stability and alkaloids analysis of acclimatized plantlets of Thalictrum foliolosum DOI
Manoj Kumar Mishra, Shatrujeet Pandey, Pratibha Misra

et al.

Plant Cell Tissue and Organ Culture (PCTOC), Journal Year: 2020, Volume and Issue: 142(2), P. 441 - 446

Published: June 2, 2020

Language: Английский

Citations

15
Assessment of direct shoot organogenesis and genetic fidelity in Solanum viarum Dunal—a commercially important medicinal plant DOI
Shatrujeet Pandey, Preeti Patel, Archana Prasad

et al.

In Vitro Cellular & Developmental Biology - Plant, Journal Year: 2020, Volume and Issue: 56(4), P. 538 - 547

Published: July 13, 2020

Language: Английский

Citations

15
Genome-Wide Association Study (GWAS) for Examining the Genomics Controlling Prickle Production in Red Raspberry (Rubus idaeus L.) DOI Creative Commons
Archana Khadgi, Courtney Weber

Agronomy, Journal Year: 2020, Volume and Issue: 11(1), P. 27 - 27

Published: Dec. 24, 2020

Red raspberry (Rubus idaeus L.) is an expanding high-value berry crop worldwide. The presence of prickles, outgrowths epidermal tissues lacking vasculature, on the canes, petioles, and undersides leaves complicates both field management harvest. utilization cultivars with fewer prickles or prickle-free canes simplifies production. A previously generated population segregating for utilizing s locus between cultivar Joan J (ss) prickled Caroline (Ss) was analyzed to identify genomic region associated prickle development in red raspberry. Genotype by sequencing (GBS) combined a genome-wide association study (GWAS) using fixed random model circulating probability unification (FarmCPU) analyze 8474 single nucleotide polymorphisms (SNPs) significant markers trait. total four SNPs were identified chromosome 4 that phenotype located near annotated genes. This demonstrates how genetics can be used decipher genetic control important horticultural traits Rubus, provides valuable information about potential genes underlying

Language: Английский

Citations

15
The Transcriptome Profiling of Flavonoids and Bibenzyls Reveals Medicinal Importance of Rare Orchid Arundina graminifolia DOI Creative Commons
Sagheer Ahmad, Jie Gao, Yonglu Wei

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: June 23, 2022

Orchids are very important flowering plants that spend long juvenile phases before flowering. Along with aesthetic importance, they rich sources of medicinal components. However, their reproductive cycle is the major hurdle to study efficacy. Arundina graminifolia a rare orchid grows fast, unlike other orchids, and this characteristic makes it an ideal plant enrichment orchids. Therefore, presents identification components in various parts A. . Transcriptome analysis was performed for five stages (FD1–FD5) flower development four tissue types (mature flower, silique, root, leaf) ascertain genetic regulators flavonoids bibenzyls. Most genes showed highest expression roots as compared tissues. Weighted gene coexpression network (WGCNA) identify modules candidate involving biosynthesis pathways these chemicals. MEyellow module contained highly coexpressed genes. Moreover, concentrations phenylpropanoid, bibenzyls, flavone were ascertained through high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Phenylpropanoid bibenzyl comparatively high leaf, while concentration stem. The selected [bibenzyl (BIBSY212), CYP84A1, CYP73A4, 4CLL7, UGT88B1, UGT73C3, anthocyanin synthase (ANS), phenylalanine ammonia-lyase (PAL), flavanone FLS, CHS8] validated quantitative real-time PCR (qRT-PCR). leaf root tissue. presence bibenzyls different molecular can provide quick source decipher efficacy

Language: Английский

Citations

9