Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(8), P. 2781 - 2781

Published: April 16, 2020

Sperm cryopreservation represents a powerful tool for livestock breeding. Several efforts have been made to improve the efficiency of sperm in different ruminant species. However, significant amount still suffers considerable cryodamage, which may affect quality and fertility. Recently, use "omics" technologies cryobiology, especially proteomics studies, has led better understanding molecular modifications induced by cryopreservation, facilitating identification freezability biomarkers certain proteins that can be added before enhance cryosurvival. This review provides an updated overview mechanisms involved are part responsible structural, functional fertility changes observed frozen-thawed sperm. Moreover, basis those factors freezing resilience species is also discussed as well aspects novel strategies developed reduce including new cryoprotectants, antioxidants, proteins, nanoparticles vitrification.

Language: Английский

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Cryopreservation of Semen in Domestic Animals: A Review of Current Challenges, Applications, and Prospective Strategies

Animals, Journal Year: 2022, Volume and Issue: 12(23), P. 3271 - 3271

Published: Nov. 24, 2022

Cryopreservation is a way to preserve germplasm with applications in agriculture, biotechnology, and conservation of endangered animals. has been available for over century, yet, using current methods, only around 50% spermatozoa retain their viability after cryopreservation. This loss associated damage different sperm components including the plasma membrane, nucleus, mitochondria, proteins, mRNAs, microRNAs. To mitigate this damage, conventional strategies use chemical additives that include classical cryoprotectants such as glycerol, well antioxidants, fatty acids, sugars, amino membrane stabilizers. However, clearly protocols do not prevent all damage. may be due imperfect function antioxidants probable conversion media more toxic forms during

Language: Английский

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Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(4), P. 3379 - 3379

Published: Feb. 8, 2023

For decades now, sperm cryopreservation has been a pillar of assisted reproduction in animals as well humans. Nevertheless, the success varies across species, seasons, and latitudes even within same individual. With dawn progressive analytical techniques field genomics, proteomics, metabolomics, new options for more accurate semen quality assessment have become available. This review summarizes currently available information on specific molecular characteristics spermatozoa that could predict their cryotolerance before freezing process. Understanding changes biology result exposure to low temperatures may contribute development implementation appropriate measures assure high post-thaw quality. Furthermore, an early prediction or cryosensitivity lead establishment customized protocols interconnecting adequate processing procedures, techniques, cryosupplements are most feasible individual needs ejaculate.

Language: Английский

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An Exploration of Current and Perspective Semen Analysis and Sperm Selection for Livestock Artificial Insemination

Animals, Journal Year: 2021, Volume and Issue: 11(12), P. 3563 - 3563

Published: Dec. 15, 2021

Artificial insemination of livestock has been a staple technology for producers worldwide over sixty years. This reproductive allowed the rapid improvement genetics, most notably in dairy cattle and pigs. field experienced continuous improvements last six decades. Though much work carried out to improve efficiency AI, there are still many areas which continue experience improvement, including semen analysis procedures, sperm selection techniques, sexing technologies, storage methods. Additionally, use AI continues grow beef cattle, horses, small ruminants as become more efficient yield higher pregnancy rates. In this review, trends various species well cutting edge aforementioned will be discussed at length. Future refine protocols used increase rates within all species.

Language: Английский

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Biomarker-based human and animal sperm phenotyping: the good, the bad and the ugly

Biology of Reproduction, Journal Year: 2024, Volume and Issue: 110(6), P. 1135 - 1156

Published: April 19, 2024

Abstract Conventional, brightfield-microscopic semen analysis provides important baseline information about sperm quality of an individual; however, it falls short identifying subtle subcellular and molecular defects in cohorts “bad,” defective human animal spermatozoa with seemingly normal phenotypes. To bridge this gap, is desirable to increase the precision andrological evaluation humans livestock animals by pursuing advanced biomarker-based imaging methods. This review, spiced up occasional classic movie references but seriously scholastic at same time, focuses mainly on biomarkers altered male germ cell proteostasis resulting post-testicular carryovers proteins associated ubiquitin-proteasome system. Also addressed are redox homeostasis, epididymal maturation, sperm–seminal plasma interactions, surface glycosylation. Zinc ion homeostasis-associated sperm-borne components, including elements neurodegenerative pathways such as Huntington Alzheimer disease, discussed. Such spectrum biomarkers, imaged highly specific vital fluorescent probes, lectins, antibodies, reveals both obvious chromatin, deoxyribonucleic acid, accessory structures head tail. Introduction next-generation image-based flow cytometry into research clinical andrology will soon enable incorporation machine deep learning algorithms end point developing simple, label-free methods for diagnostics high-throughput phenotyping economically animals.

Language: Английский

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ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm

Biomolecules, Journal Year: 2020, Volume and Issue: 10(7), P. 1046 - 1046

Published: July 14, 2020

To improve artificial insemination protocols in ovine species it is crucial to optimize sperm quality evaluation after preservation technologies. Emerging technologies based on novel biomolecules and related redox balance proteins involved motility such as ProAKAP4 could be successfully applied ram evaluation. In this work, a multiparametric analysis of fresh, cooled, cryopreserved was performed at different complexity levels. Samples were evaluated terms (total motility, progressive curvilinear velocity), viability, apoptosis, content reactive oxygen species, oxidation‒reduction potential, expression concentration. As expected, samples showed significant decrease (p < 0.05), evidencing freezability classes among that detected by analyses. However, cooled no differences found concerning ROS content, compared fresh explain the reported fertility rates. although proportion positive-cells remained unaltered control, concentration protein significantly decreased 0.05) samples. This altered level contribute rates some authors. More importantly, can established promising diagnostic parameter allowing us conservation finally species.

Language: Английский

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An updated review on the application of proteomics to explore sperm cryoinjury mechanisms in livestock animals

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 263, P. 107441 - 107441

Published: Feb. 21, 2024

Language: Английский

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Effect of age-associated oxidative stress on sperm viability, acrosomal integrity and sperm apoptosis and DNA fragmentation in Sahiwal breeding bulls

Veterinary Research Communications, Journal Year: 2025, Volume and Issue: 49(2)

Published: Jan. 11, 2025

Language: Английский

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Genome-wide discovery of underlying genetic factors associated with fresh and frozen-thawed semen traits in composite ram breeds exhibiting different cryotolerance

Cryobiology, Journal Year: 2025, Volume and Issue: 118, P. 105197 - 105197

Published: Jan. 18, 2025

Language: Английский

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Overcoming ice: cutting-edge materials and advanced strategies for effective cryopreservation of biosample

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 7, 2025

Cryopreservation techniques have been widely used, especially in biomedical applications and preservation of germplasm resources. Ideally, biological materials would maintain functional integrity as well a normal structure can be recovered when needed. However, this tool does not work all the time. Ice formation growth are key challenges. The other major reason is that cryoprotective agents (CPAs) currently used do meet these needs always accompanied by their cytotoxicity. A comprehensive synergistic approach focuses on overall frozen system crucial for evolution cryopreservation methods. In review, we first summarize fundamental damage mechanisms during cryopreservation, common cryoprotectants limitations. Next, discuss interact with ice to improve outcomes. We evaluated natural synthetic materials, including sugars polymers, AFPs mimics, nucleators, hydrogels. addition, biochemical regulation, which enhances tolerance biosamples cryopreservation-induced stresses, was also mentioned. Nanotechnology, cell encapsulation, cryomesh, isochoric freezing, such scalable approaches, further discussed cryopreservation. Finally, future research directions field efficient proposed. emphasized need multidisciplinary progress address combination cryobiology technologies, biology, nanotechnology, microfluidics, 3D bioprinting, highlighted.

Language: Английский

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