The rearing environment persistently modulates mouse phenotypes from the molecular to the behavioural level DOI Creative Commons
Ivana Jarić, Bernhard Voelkl, Mélanie Clerc

et al.

PLoS Biology, Journal Year: 2022, Volume and Issue: 20(10), P. e3001837 - e3001837

Published: Oct. 21, 2022

The phenotype of an organism results from its genotype and the influence environment throughout development. Even when using animals same genotype, independent studies may test different phenotypes, resulting in poor replicability due to genotype-by-environment interactions. Thus, genetically defined strains mice respond differently experimental treatments depending on their rearing environment. However, extent such phenotypic plasticity implications for research findings have remained unknown. Here, we examined which common environmental differences between animal facilities modulate homogeneous (inbred) mice. We conducted a comprehensive multicentre study, whereby inbred C57BL/6J single breeding cohort were allocated reared 5 early life adolescence, before being transported laboratory. found persistent effects facility composition heterogeneity gut microbial community. These paralleled by body weight behavioural Furthermore, show that variation among is strong enough epigenetic patterns neurons at level chromatin organisation. detected changes organisation regulatory regions genes involved nucleosome assembly, neuronal differentiation, synaptic plasticity, regulation behaviour. Our demonstrate produce facility-specific molecular level. they highlight important limitation inferences single-laboratory thus argue study designs should take background into account increase robustness findings.

Language: Английский

Potential of microalgae as a sustainable feed ingredient for aquaculture DOI Creative Commons
Senthil Nagappan, Probir Das, Mohammed AbdulQuadir

et al.

Journal of Biotechnology, Journal Year: 2021, Volume and Issue: 341, P. 1 - 20

Published: Sept. 15, 2021

An increase in fish consumption, combined with a decrease wild harvest, is driving the aquaculture industry at rapid pace. Today, farmed seafood accounts for about half of all global demand human consumption. As continues to grow, so does market aquafeed. Currently, some feed ingredients are coming from low-value forage fishes (fish meal) and terrestrial plants. The production meal can't be increased as it would affect sustainability ecosystem ocean. Similarly, increasing plant-based leads deforestation freshwater use. Hence, alternative environmentally sustainable sources need developed. Microalgae biomasses represent potential source cell metabolites these microorganisms contain blend essential amino acids, healthy triglycerides fat, vitamins, pigments. In addition serving bulk ingredient aquafeed, their unique array bioactive compounds can survivability species, improve coloration quality fillet. has highest areal biomass productivities among photosynthetic organisms, including fodder crops, thus high commercial potential. Also, microalgal low water arable-land footprint, making microalgal-based sustainable. This review paper will explore producing microalgae an feed.

Language: Английский

Citations

263
Gold nanoparticles (GNPs) in biomedical and clinical applications: A review DOI
Muzahidul I. Anik, Niaz Mahmud, Abdullah Al Masud

et al.

Nano Select, Journal Year: 2021, Volume and Issue: 3(4), P. 792 - 828

Published: Oct. 13, 2021

Abstract Gold nanoparticles (GNPs) have been extensively used in various applications ranging from environmental detection to biomedical applications. Due their unique characteristics such as tunable surface properties well plasmon resonance (SPR), GNPs garnered attention exclusively therapy and diagnostics. Their ease of synthesis compatibility with functionalizing ligands has made them efficient a robust platform. flexibility functionalization, profoundly cancer treatment as, antiviral, antibacterial agents. In addition, owing possessing optical properties, utilized molecular imaging contrast agent. This article discusses highlights special that exploited recent years improve research field nuclear medicine, agent, vaccine development, medical implant, diagnostics, biosensing, lab‐on‐chip Moreover, size dependent biocompatibility, biodistribution, excreation was discussed details for vivo At the forefront modern theraputic technology, based antiviral agents great potential which is also highlighted briefly this article. current state ongoing clinical trials challenges associated regulatory approval are explored. Lastly, sheds light on findings toxicity prospects future direct research.

Language: Английский

Citations

139
Fusobacterium nucleatum is associated with worse prognosis in Lauren’s diffuse type gastric cancer patients DOI Creative Commons

Ellen Teresa Boehm,

Cosima Thon,

Juozas Kupčinskas

et al.

Scientific Reports, Journal Year: 2020, Volume and Issue: 10(1)

Published: Oct. 1, 2020

Abstract Fusobacterium nucleatum ( F. ) is frequently detected in primary colorectal cancer (CRC) and matching metastasis, has been linked to a worse prognosis. We investigated the presence of gastric (GC) preneoplastic conditions stomach, its potential prognostic value GC patients. spp. were quantified various specimens from gastrointestinal tract including paired CRC tissues using probe-based qPCR. more found tumorous tissue compared non-tumorous tissues. The frequency bacterial load higher positivity showed no association chronic gastritis or such as intestinal metaplasia. -positivity was associated with significantly overall survival patients Lauren’s diffuse type, but not type GC. There gender, Helicobacter pylori -status, tumor stage localization. However, positively patient’s age trend for lower global long interspersed element-1 DNA methylation. In conclusion, our work provides novel evidence clinical relevance by showing an between prognosis Laurens’s cancer. Further studies are necessary explore related mechanistic insights therapeutic benefit targeted antibiotic treatment

Language: Английский

Citations

98
Close to the edge: Heterochromatin at the nucleolar and nuclear peripheries DOI

Aizhan Bizhanova,

Paul D. Kaufman

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, Journal Year: 2020, Volume and Issue: 1864(1), P. 194666 - 194666

Published: Dec. 8, 2020

Language: Английский

Citations

82
Autism spectrum disorder and pupillometry: A systematic review and meta-analysis DOI
Lyssa de Vries,

Iris Fouquaet,

Bart Boets

et al.

Neuroscience & Biobehavioral Reviews, Journal Year: 2020, Volume and Issue: 120, P. 479 - 508

Published: Oct. 23, 2020

Language: Английский

Citations

76
Ten Points to Improve Reproducibility and Translation of Animal Research DOI Creative Commons
Rainer Spanagel

Frontiers in Behavioral Neuroscience, Journal Year: 2022, Volume and Issue: 16

Published: April 21, 2022

Findings from animal experiments are often difficult to transfer humans. In this perspective article I discuss two questions. First, why the results of so humans? And second, what can be done improve translation Translation failures result poor methodology. It is not merely fact that low statistical power basic and preclinical studies undermine a "real effect," but accuracy with which data collected described, resulting robustness generally very does allow much more heterogeneous human condition. Equally important vast majority publications in biomedical field last few decades have reported positive findings thus generated knowledge bias. Further contributions reproducibility discussed paper, 10 points recommendation outlined. These recommendations are: (i) prior planning an actual study, systematic review or potential meta-analysis should considered. (ii) An priori calculation carried out. (iii) The experimental study protocol pre-registered. (iv) execution accordance most recent ARRIVE guidelines. (v) When generalizability also considered (e.g., sex age differences). (vi) "Method-hopping" avoided, meaning it necessary use advanced technology rather applied methodology under control. (vii) National international networks carry out multicenter obtain convergent evidence. (viii) Animal models capture DSM-5 ICD-11 criteria context research on psychiatric disorders. (ix) Raw publication made publicly available FAIR Guiding Principles for scientific management. (x) Finally, negative published counteract application these recommendation, especially confirmatory some degree exploratory studies, will ultimately research.

Language: Английский

Citations

45
Nisin lantibiotic prevents NAFLD liver steatosis and mitochondrial oxidative stress following periodontal disease by abrogating oral, gut and liver dysbiosis DOI Creative Commons
Ryutaro Kuraji, Changchang Ye, Chuanjiang Zhao

et al.

npj Biofilms and Microbiomes, Journal Year: 2024, Volume and Issue: 10(1)

Published: Jan. 17, 2024

Abstract Oral microbiome dysbiosis mediates chronic periodontal disease, gut microbial dysbiosis, and mucosal barrier disfunction that leads to steatohepatitis via the enterohepatic circulation. Improving this towards health may improve liver disease. Treatment with antibiotics probiotics have been used modulate microbial, immunological, clinical landscape of disease some success. The aim present investigation was evaluate potential for nisin, an antimicrobial peptide produced by Lactococcus lactis , counteract periodontitis-associated glycolipid-metabolism inflammation in liver. Periodontal pathogens, namely Porphyromonas gingivalis Treponema denticola Tannerella forsythia Fusobacterium nucleatum were administrated topically onto oral cavity establish polymicrobial mice. In context nisin treatment significantly shifted a new composition, commensurate while preventing harmful small intestine concomitant decreased villi structural integrity, heightened hepatic exposure bacteria lipid malondialdehyde accumulation Validation RNA Seq analyses, confirmed significant infection-related alteration several genes involved mitochondrial dysregulation, oxidative phosphorylation, metal/iron binding their restitution following treatment. support these vivo findings indicating periodontopathogens induce gastrointestinal distant organ lesions, human autopsy specimens demonstrated correlation between tooth loss severity Nisin’s ability shift state mitigating enteritis, represents novel approach treating NAFLD-steatohepatitis-associated

Language: Английский

Citations

15
Challenging current scientific practice: how a shift in research methodology could reduce animal use DOI Creative Commons
S. Helene Richter

Lab Animal, Journal Year: 2024, Volume and Issue: 53(1), P. 9 - 12

Published: Jan. 1, 2024

The 3R principles provide an ethical framework for animal research throughout the world. However, despite increasing awareness of these principles, there is still a lot room improving their implementation, especially when it comes to reduction. By combining Bayesian statistics with shift in experimental design, here we present entirely new idea reduce numbers within experiments.

Language: Английский

Citations

12
Inhibition of the BTK-IDO-mTOR axis promotes differentiation of monocyte-lineage dendritic cells and enhances anti-tumor T cell immunity DOI Creative Commons
Madhav Sharma, Rafał Pacholczyk, Huidong Shi

et al.

Immunity, Journal Year: 2021, Volume and Issue: 54(10), P. 2354 - 2371.e8

Published: Oct. 1, 2021

Language: Английский

Citations

54
Use of dried blood spot samples for SARS-CoV-2 antibody detection using the Roche Elecsys ® high throughput immunoassay DOI Creative Commons
Ranya Mulchandani, Ben Brown, Tim Brooks

et al.

Journal of Clinical Virology, Journal Year: 2021, Volume and Issue: 136, P. 104739 - 104739

Published: Jan. 19, 2021

Dried blood spot samples (DBS) provide an alternative sample type to venous for antibody testing. DBS are used by NHS diagnosing Hepatitis C and Public Health England large scale HIV serosurveillance; the applicability of based approaches SARS-CoV-2 detection is uncertain. The study aimed compare in eluates using Roche Elecsys ® immunoassay with paired plasma samples, same assay. was one Police Fire & Rescue facility England; it comprised 195 participants within a larger COVID-19 serodiagnostics keyworkers, EDSAB-HOME. Outcome measures were sensitivity specificity (the index test) relative reference test), at experimental cut-off; quality collected; estimates vs. population. 18/195 (9.2%) tested positive samples. varied markedly phlebotomist, low volume significantly reduced signals. Using cut-off, 89.0% (95% CI 67.2, 96.9%) 100.0% 97.9, 100%) respectively compared plasma. limit about 30 times higher than use serology, though feasible, insensitive immunoassays on Sample impacts assay performance. Alternatives, including collection capillary should be considered screening programs.

Language: Английский

Citations

53