Phytochemical investigation, antibacterial, and ameliorative potential effects of Tamarix nilotica on LPS-induced acute lung injury model in mice DOI Open Access
Rasha Assiri,

Thanaa A. El‐Masry,

Sahar M. El-Haggar

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 168, P. 115678 - 115678

Published: Oct. 9, 2023

Acute lung injury (ALI) is a serious illness with high mortality rate of 40-60%. It characterised by systemic inflammatory processes and oxidative stress. Gram-negative bacterial infections are the major cause ALI, lipopolysaccharide (LPS) stimulus for release mediators. Hence, there an urgent need to develop new therapies which ameliorate ALI prevent its consequences. The Middle Eastern native plant Tamarix nilotica (Ehrenb) Bunge belongs family Tamaricaceae, exhibits strong anti-inflammatory antioxidant effects. Thus, current work aimed ensure plausible beneficial effects T. different fractions on LPS-induced acute after elucidating their phytochemical constituents using LC/MS analysis. Mice were randomly allocated into six groups: Control saline, LPS group, four groups treated total extract, DCM, EtOAc n-butanol fractions, respectively, intraperitoneal at 100 mg/kg doses 30 min before injection. expression iNOS, TGF-β1, NOX-1, NOX-4 GPX-1 levels evaluated. Also, stress was assessed via measurements MDA, SOD Catalase activity, histopathological immunohistochemical investigation TNF-α in tissues performed. fraction caused significant downregulation TNF-α, NOX-4, MDA (p ˂ 0.05), significantly elevated levels, SOD, catalase activity alleviated all abnormalities confirming advantageous role ALI. antibacterial activities investigated agar well diffusion method broth microdilution method. Interestingly, exhibited best against Klebsiella pneumoniae clinical isolates. also reduced exopolysaccharide quantity, cell surface hydrophobicity, biofilm formation.

Language: Английский

Characterization of newly isolated bacteriophage to control multi-drug resistant Pseudomonas aeruginosa colonizing incision wounds in a rat model: in vitro and in vivo approach DOI

Amal M. Abo Kamer,

Ahmed Abdelaziz,

Ahmed M. Nosair

et al.

Life Sciences, Journal Year: 2022, Volume and Issue: 310, P. 121085 - 121085

Published: Oct. 17, 2022

Language: Английский

Citations

11
Antibacterial and anti-biofilm activity of diarylureas against Enterococcus faecium by suppressing the gene expression of peptidoglycan hydrolases and adherence DOI Creative Commons

Yunfeng Xie,

Lei Wang, Yang Yang

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Dec. 15, 2022

Enterococcus faecium (E. faecium) is a clinical multidrug-resistant pathogen causing life-threatening infection, which makes it important to discover antibacterial agents with novel scaffolds and unique mechanism. In this study, the diarylurea scaffold was found have potent effect on E. faecium. Diarylurea ZJ-2 benign drug-like property exhibited anti-biofilm activity through inhibiting genes expression of NlpC/p60 hydrolase-secreted antigen A (sagA) autolysins (atlA), down-regulating biofilm adherence related aggregation substance (agg), enterococcal surface protein (esp) against Moreover, can be docked into SagA inhibit daughter cell separation. mouse model abdominal decreased bacterial load level IL-6 TNF-α in time-dependent manner. Overall, these findings indicated that has potential developed as therapeutic agent treat drug-resistant enterococci biofilm-related infections.

Language: Английский

Citations

11
Exploring the potential efficacy of phage therapy for biocontrol of foodborne pathogenic extensively drug-resistant Escherichia coli in gastrointestinal tract of rat model DOI
Ahmed Abdelaziz,

Amal M. Abo Kamer,

Ahmed M. Nosair

et al.

Life Sciences, Journal Year: 2023, Volume and Issue: 315, P. 121362 - 121362

Published: Jan. 4, 2023

Language: Английский

Citations

6
Antibacterial and Antibiofilm Effects of Allelopathic Compounds Identified in Medicago sativa L. Seedling Exudate against Escherichia coli DOI Creative Commons
Sara Abouzeid,

Ulrike Beutling,

Engy Elekhnawy

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(6), P. 2645 - 2645

Published: March 14, 2023

In this study, the allelopathic properties of Medicago sativa L. (alfalfa) seedling exudates on germination seeds various species were investigated. The compounds responsible for effects alfalfa identified and characterized by employing liquid chromatography ion mobility high-resolution mass spectrometry. Crude inhibited all plant tested. Overall, nine in quantified. most predominant a hyperoside representing flavonoid glucoside, non-proteinogenic amino acid canavanine, two dipeptides, as H-Glu-Tyr-OH H-Phe-Glu-OH. latter corresponds to first finding that dipeptides are exuded from seedlings. addition, antibacterial antibiofilm activities exudate its elucidated. Both canavanine revealed best activity with minimum inhibitory concentration (MIC) values ranged 8 32 256 µg/mL, respectively. Regarding action, caused decline percentage E. coli isolates possessed strong moderate biofilm-forming potential 68.42% 21.05% 31.58%, Studies their inhibiting exhibit these major substances predominantly antimicrobial crude exudates.

Language: Английский

Citations

5
Phytochemical investigation, antibacterial, and ameliorative potential effects of Tamarix nilotica on LPS-induced acute lung injury model in mice DOI Open Access
Rasha Assiri,

Thanaa A. El‐Masry,

Sahar M. El-Haggar

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 168, P. 115678 - 115678

Published: Oct. 9, 2023

Acute lung injury (ALI) is a serious illness with high mortality rate of 40-60%. It characterised by systemic inflammatory processes and oxidative stress. Gram-negative bacterial infections are the major cause ALI, lipopolysaccharide (LPS) stimulus for release mediators. Hence, there an urgent need to develop new therapies which ameliorate ALI prevent its consequences. The Middle Eastern native plant Tamarix nilotica (Ehrenb) Bunge belongs family Tamaricaceae, exhibits strong anti-inflammatory antioxidant effects. Thus, current work aimed ensure plausible beneficial effects T. different fractions on LPS-induced acute after elucidating their phytochemical constituents using LC/MS analysis. Mice were randomly allocated into six groups: Control saline, LPS group, four groups treated total extract, DCM, EtOAc n-butanol fractions, respectively, intraperitoneal at 100 mg/kg doses 30 min before injection. expression iNOS, TGF-β1, NOX-1, NOX-4 GPX-1 levels evaluated. Also, stress was assessed via measurements MDA, SOD Catalase activity, histopathological immunohistochemical investigation TNF-α in tissues performed. fraction caused significant downregulation TNF-α, NOX-4, MDA (p ˂ 0.05), significantly elevated levels, SOD, catalase activity alleviated all abnormalities confirming advantageous role ALI. antibacterial activities investigated agar well diffusion method broth microdilution method. Interestingly, exhibited best against Klebsiella pneumoniae clinical isolates. also reduced exopolysaccharide quantity, cell surface hydrophobicity, biofilm formation.

Language: Английский

Citations

4