Drug delivery strategies for antibiofilm therapy

Nature Reviews Microbiology, Journal Year: 2023, Volume and Issue: 21(9), P. 555 - 572

Published: May 31, 2023

Language: Английский

---

Bacterial biofilm inhibitors: An overview

Ecotoxicology and Environmental Safety, Journal Year: 2023, Volume and Issue: 264, P. 115389 - 115389

Published: Aug. 25, 2023

Bacteria that cause infectious diseases adopt biofilms as one of their most prevalent lifestyles. Biofilms enable bacteria to tolerate environmental stress and evade antibacterial agents. This bacterial defense mechanism has rendered the use antibiotics ineffective for treatment diseases. However, many highly drug-resistant microbes have rapidly emerged owing such treatments. Different signaling mechanisms regulate biofilm formation, including cyclic dinucleotide (c-di-GMP), small non-coding RNAs, quorum sensing (QS). A cell density-dependent phenomenon, QS is associated with c-di-GMP (a global messenger), which regulates gene expression related adhesion, extracellular matrix production, transition from planktonic stage, stability, pathogenicity, virulence, acquisition nutrients. The article aims provide information on inhibiting formation disintegrating mature/preformed biofilms. enables antimicrobials target free-living/exposed cells at lower concentrations than those needed treat within biofilm. Therefore, a complementary action antibiofilm antimicrobial agents can be robust strategic approach dealing Taken together, these molecules broad implications human health.

Language: Английский

---

Niosomes for nose-to-brain delivery: A non-invasive versatile carrier system for drug delivery in neurodegenerative diseases

Journal of Drug Delivery Science and Technology, Journal Year: 2023, Volume and Issue: 89, P. 105007 - 105007

Published: Sept. 28, 2023

Language: Английский

---

Niosome as an Effective Nanoscale Solution for the Treatment of Microbial Infections

BioMed Research International, Journal Year: 2023, Volume and Issue: 2023(1)

Published: Jan. 1, 2023

Numerous disorders go untreated owing to a lack of suitable drug delivery technology or an appropriate therapeutic moiety, particularly when toxicities and side effects are major concern. Treatment options for microbiological infections not fulfilled significant adverse extended options. Advanced therapy options, such as active targeting, may be preferable traditional ways treating infectious diseases. Niosomes can defined microscopic lamellar molecules formed by mixture cholesterol, nonionic surfactants (alkyl dialkyl polyglycerol ethers), sometimes charge-inducing agents. These comprise both hydrophilic hydrophobic moieties varying solubilities. In this review, several pathogenic microbes Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Plasmodium, Leishmania, Candida spp. have been evaluated. Also, the development proper niosomal formulation required application was discussed. This review also reviews that optimal is dependent on aspects, including choice surfactant, fabrication process, parameters. Finally, will give information effectiveness niosomes in acute microbial infections, mechanism action combating pathogens, advantages using over other treatment modalities.

Language: Английский

---

3D Printing of Alginate/Chitosan-Based Scaffold Empowered by Tyrosol-Loaded Niosome for Wound Healing Applications: In Vitro and In Vivo Performances

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(3), P. 1449 - 1468

Published: March 5, 2024

This study introduces a tyrosol-loaded niosome integrated into chitosan–alginate scaffold (Nio-Tyro@CS-AL), employing advanced electrospinning and 3D printing techniques for wound healing applications. The niosomes, measuring 185.40 ± 6.40 nm with polydispersity index of 0.168 0.012, encapsulated tyrosol an efficiency 77.54 1.25%. scaffold's microsized porous structure (600–900 μm) enhances water absorption, promoting cell adhesion, migration, proliferation. Mechanical property assessments revealed the enhanced resilience, niosomes increasing compressive strength, modulus, strain to failure, indicative its suitability healing. Controlled release was demonstrated in vitro, essential therapeutic efficacy. exhibited significant antibacterial activity against Pseudomonas aeruginosa Staphylococcus aureus, substantial biofilm inhibition downregulation bacterial genes (ndvb icab). A assay highlighted notable increase MMP-2 MMP-9 mRNA expression closure area (69.35 2.21%) HFF cells treated Nio-Tyro@CS-AL. In vivo studies mice confirmed biocompatibility, showing no inflammatory response, hypertrophic scarring, or foreign body reaction. Histological evaluations increased fibroblast macrophage activity, re-epithelialization, angiogenesis wounds Nio-Tyro@CS-AL, indicating effective tissue integration repair. Overall, Nio-Tyro@CS-AL presents advancement wound-healing materials, combining properties regeneration, holds promising potential clinical applications management.

Language: Английский

---

Antibacterial and antibiofilm potentials of vancomycin-loaded niosomal drug delivery system against methicillin-resistant Staphylococcus aureus (MRSA) infections

BMC Biotechnology, Journal Year: 2024, Volume and Issue: 24(1)

Published: July 8, 2024

Abstract The threat of methicillin-resistant Staphylococcus aureus (MRSA) is increasing worldwide, making it significantly necessary to discover a novel way dealing with related infections. quick spread MRSA isolates among infected individuals has heightened public health concerns and limited treatment options. Vancomycin (VAN) can be applied treat severe infections, the indiscriminate administration this antimicrobial agent caused several in medical settings. Owing advantageous characteristics, niosomal drug delivery system may increase potential loaded agents. This work aims examine antibacterial anti-biofilm properties VAN-niosome against clinical emphasis on cytotoxicity stability studies. Furthermore, we aim suggest an effective approach infections by investigating inhibitory effect formulated niosome expression biofilm-associated gene ( icaR ). thin-film hydration was used prepare (Tween 60, Span cholesterol), field emission scanning electron microscopy (FE-SEM), vitro release, dynamic light scattering (DLS), entrapment efficiency (EE%) were investigate physicochemical properties. physical VAN-niosome, including hydrodynamic size, polydispersity index (PDI), EE%, analyzed for 30-day storage time at 4 °C 25 °C. In addition, human foreskin fibroblast (HFF) cell line evaluate cytotoxic synthesized niosome. Moreover, minimum bactericidal concentrations (MICs/MBCs) assess VAN formulation. Also, antibiofilm investigated microtiter plate (MTP) real-time PCR methods. FE-SEM result revealed that had spherical morphology. size PDI reported DLS method 201.2 nm 0.301, respectively. surface zeta charge prepared − 35.4 mV, EE% ranged between 58.9 62.5%. release study sustained-release profile Our showed acceptable during time. Additionally, stronger compared free VAN. conclusion, our demonstrated could promising as successful due sustained negligible toxicity, high encapsulation capacity. studies capacity isolates. results exhibited proposed powerful strategy biofilm via down-regulation expression.

Language: Английский

---

CRISPR/Cas9-mediated LINC00511 knockout strategies, increased apoptosis of breast cancer cells via suppressing antiapoptotic genes

Biological Procedures Online, Journal Year: 2022, Volume and Issue: 24(1)

Published: July 5, 2022

The growing detection of long noncoding RNAs (lncRNAs) required the application functional approaches in order to provide absolutely precise, conducive, and reliable processed information along with effective consequences. We utilized genetic knockout (KO) techniques ablate Long Intergenic Noncoding RNA 00,511 gene several humans who suffered from breast cancer cells at end we analyzed examined results.The predictive relevance LINC00511 expression pattern was measured by using a pooled hazard ratio (HR) 95% confidence interval (CI). link among profiles metastasis odds (OR) interval. This meta- analysis composed fifteen studies which contained total 1040 tumor patients. used three distinct CRISPR/Cas9-mediated knockdown prevent lncRNA being transcribed. RT-PCR measure expression. CCK-8, colony formation tests, invasion transwell test cell proliferation invasion. stemness sphere-formation test. To validate molecular attachment, luciferase reporter assays were performed. impacts deletion lines confirmed RT-qPCR, MTT, meta-analysis 15 trials malignant tumors. Greater ascribed lower overall survival (HR = 1.93, CI 1.49-2.49, < P 0.001) an increased proportion lymph node (OR 3.07, 2.23-4.23, meta-analysis. It found that role overexpressed samples, this overexpression poor prognosis. gain loss-of-function tests demonstrated findings such as proliferation, sphere-forming ability, growth. Additionally, transcription factor E2F1 binds Nanog gene's promoter site induce transcription. P57, P21, Prkca, MDM4, Map2k6, FADD treatment group (LINC00511 deletion) significantly higher than control (P 0.01). In addition, had BCL2 surviving genes 0.001). each two target alleles, du-HITI approach introduced termination signal. strategy's donor vector preparation easier "CRISPR HDR," selection likewise much excision." Furthermore, when initial transfection attempt, single-cell knockouts for both alleles generated.The methods employed described work could be extended production and, theory, other lncRNAs study their function.

Language: Английский

---

Identification of Antigenic Properties of Acinetobacter baumannii Proteins as Novel Putative Vaccine Candidates Using Reverse Vaccinology Approach

Applied Biochemistry and Biotechnology, Journal Year: 2022, Volume and Issue: 194(10), P. 4892 - 4914

Published: June 7, 2022

Language: Английский

---

Novel Niosome-Encapsulated 2,5-Diketopiperazine (BHPPD): Synthesis, Formulation, and Anti-breast Cancer Activity

Applied Biochemistry and Biotechnology, Journal Year: 2023, Volume and Issue: 196(6), P. 3126 - 3147

Published: Aug. 25, 2023

Language: Английский

---

Composition, preparation methods, and applications of nanoniosomes as codelivery systems: a review of emerging therapies with emphasis on cancer

Nanoscale, Journal Year: 2023, Volume and Issue: 16(6), P. 2713 - 2746

Published: Dec. 7, 2023

Nanoniosome-based drug codelivery systems have become popular therapeutic instruments, demonstrating tremendous promise in cancer therapy, infection treatment, and other domains. An emerging form of vesicular nanocarriers, niosomes are self-assembling vesicles composed nonionic surfactants, along with cholesterol or amphiphilic molecules. This comprehensive review focuses on how nanosystems may aid making anticancer antibacterial pharmaceuticals more stable soluble. As malleable nanodelivery the composition, types, preparation procedures, variables affecting structure stability extensively investigated. In addition, advantages dual for combination therapy administration multiple medications simultaneously highlighted. Along categorizing niosomal delivery systems, a analysis various techniques, including thin-layer injection, ether microfluidization, is provided. Dual treatment discussed detail regarding two organic, plant-based bioactive compounds gene agents. niogelosomes metallic carriers targeted distribution discussed. The also investigates simultaneous substances agents, siRNA, microRNA, shRNA, lncRNA, DNA. Additional sections discuss use cutaneous treating leishmanial infections,

Language: Английский

---