Hairy root induction and plant regeneration techniques in Brassicaceae for biotechnological applications DOI Creative Commons
Veronika Jedličková, Marie Štefková, Terezie Mandáková

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 28, 2023

Abstract Background Hairy roots constitute a valuable tissue culture system for species that are difficult to propagate through conventional seed-based methods. Moreover, the generation of transgenic plants can be facilitated by employing carefully designed hormone-containing media. Results We initiated hairy root formation in rare crucifer Asperuginoides axillaris via an injection-based protocol and successfully regenerated from established lines. Our study confirms genetic stability both their derived regenerants highlights utility as permanent source mitotic chromosomes cytogenetic investigations. Additionally, we have developed effective embryo rescue circumvent seed dormancy issues A. seeds. By using inflorescence primary stems Arabidopsis thaliana Cardamine hirsuta starting material, also lines, were subsequently used regeneration studies. Conclusion efficient transformation protocols various crucifers, namely , C. . serve continuous plant material molecular analyses.

Language: Английский

Seed Science and Technology DOI Creative Commons

Malavika Dadlani,

Devendra Kumar Yadava

Published: Jan. 1, 2023

This open access edited book is a collection of 21 chapters synthesized from nearly 100 lectures by >70 eminent international and Indian experts.

Language: Английский

Citations

824
Seed Dormancy and Regulation of Germination DOI Creative Commons
Prakash Nautiyal,

K. Sivasubramaniam,

Malavika Dadlani

et al.

Seed Science and Technology, Journal Year: 2023, Volume and Issue: unknown, P. 39 - 66

Published: Jan. 1, 2023

Abstract Seed germination and dormancy are vital components of seed quality; hence, understanding these processes is essential for a sound production system. The two closely interrelated regulated, both by genetic as well environmental factors. While provides an inherent mechanism aimed at the survival plant species to withstand adverse external conditions restricting mature from germinating, ability dehydrated remain viable produce vigorous seedling upon hydration under favourable key perpetuation species. In addition, quality expected result in timely uniform field after sowing establish healthy crop stand. Therefore, technology, not considered desirable trait lots used sowing. Thus, achieve highest percentage, factors controlling interlinked contrasting vital. testing trade, knowledge needed reliable assessment its planting value, make right decisions. Though much yet be understood, present status on aspects has made significant advances, especially control, molecular mechanism, physiological influencing dormancy. information compiled this chapter may help technologists developing new methods breaking germination,

Language: Английский

Citations

32
Optimizing Hydropriming Duration for Enhanced Germination, Emergence and Seedling Growth of Ocimum gratissimum: A Path to Sustainable Production in Nigeria DOI
J. O. Adinde,

N. C. Enemuo,

O.C. Okeke

et al.

Asian Journal of Biological Sciences, Journal Year: 2025, Volume and Issue: 18(1), P. 229 - 236

Published: Jan. 2, 2025

Language: Английский

Citations

0
Light environment control for reducing energy loss and increasing crop yield in plant factories DOI

Huichuan Zou,

Chunzhe Li,

Aoyu Zhang

et al.

Solar Energy, Journal Year: 2023, Volume and Issue: 268, P. 112281 - 112281

Published: Dec. 28, 2023

Language: Английский

Citations

10
Chemical Seed Priming: Molecules and Mechanisms for Enhancing Plant Germination, Growth, and Stress Tolerance DOI Creative Commons
Mason T. MacDonald,

V. Mohan

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(3), P. 177 - 177

Published: March 7, 2025

Food security is one of the world’s top challenges, specifically considering global issues like climate change. Seed priming strategy to improve crop production, typically via increased germination, yields, and/or stress tolerance. Hydropriming, or soaking seeds in water only, simplest form seed priming. However, addition certain agents has resulted a variety modified strategies, including osmopriming, halopriming, hormonal priming, PGR nutripriming, and others. Most current research focused on nutripriming. This review will focus specific compounds that have been used most often over past 3 years physiological effects they had crops. Over half recent four compounds: (1) salicylic acid, (2) zinc, (3) gibberellic (4) potassium nitrate. One interesting characteristics all chemical are exposed only yet confer benefits throughout plant development. In some cases, such passed subsequent generations, suggesting an epigenetic effect, which supported by observed changes DNA methylation histone modification. summarize state knowledge molecular mechanisms associated with discuss avenues for future research.

Language: Английский

Citations

0
Nano-Organic Coatings Improve Early Vigor of Brassica napus L. Seeds in Water Deficit DOI Creative Commons

Farkhondeh Abrahimi,

Mansour Taghvaei, Andrea Mastinu

et al.

Agronomy, Journal Year: 2023, Volume and Issue: 13(2), P. 390 - 390

Published: Jan. 28, 2023

Low emergence and vigor of canola seeds are common problems encountered by growers. Seed coating is a good way to solve this problem. The objective study was find the best combination nano-organic be used as for strengthening absorption water seed low canola. treatments in first experiment included six levels organic coatings: 20% vermicompost with 80% bentonite, 30% 70% 40% 60% residues resides bentonite. results indicated that had highest moisture under saturation conditions kept longer time. Subsequently, factors second three stress (100, 75, 50% field capacity (FC)), (control, vermicompost, residues), titanium dioxide nanoparticles (0, 0.5, 1 mM). showed improved percentage seedling traits while increasing superoxide dismutase, peroxidase, catalase activities decreasing electrolyte leakage. ratios (74.67), index (264.58), leaf area (418) were obtained at level nano-titanium (1 rates dismutase (53.44), peroxidase (30.68), (5.35), chlorophyll content (51.05), lowest leakage (42.39) observed Based on results, we suggest use rapeseed nano oxide coating. current coatings useful reducing stresses germination stages.

Language: Английский

Citations

7
Injection-based hairy root induction and plant regeneration techniques in Brassicaceae DOI Creative Commons
Veronika Jedličková, Marie Štefková, Terezie Mandáková

et al.

Plant Methods, Journal Year: 2024, Volume and Issue: 20(1)

Published: Feb. 17, 2024

Abstract Background Hairy roots constitute a valuable tissue culture system for species that are difficult to propagate through conventional seed-based methods. Moreover, the generation of transgenic plants derived from hairy can be facilitated by employing carefully designed hormone-containing media. Results We initiated root formation in rare crucifer Asperuginoides axillaris via an injection-based protocol using Agrobacterium strain C58C1 harboring root-inducing ( Ri ) plasmid and successfully regenerated established lines. Our study confirms genetic stability both their regenerants highlights utility as permanent source mitotic chromosomes cytogenetic investigations. Additionally, we have developed effective embryo rescue circumvent seed dormancy issues A. seeds. By inflorescence primary stems Arabidopsis thaliana Cardamine hirsuta starting material, also lines were subsequently used regeneration studies. Conclusion efficient transformation protocols various crucifers, namely , C. . serve continuous plant material molecular analyses.

Language: Английский

Citations

2
Assessing light spectrum impact on growth and antioxidant properties of basil family microgreens DOI Creative Commons

Akira Thongtip,

Kriengkrai Mosaleeyanon,

Supattana Janta

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 13, 2024

Understanding the influence of light spectra on plant growth and antioxidant activities is crucial for optimizing cultivation practices enhancing crop quality. In this study, we investigated effects different treatments parameters in five species: peppermint, Thai basil, cumin, lemon green holy basil. Our results revealed distinct responses to varying spectra, with consistently promoting taller heights across all species. Additionally, blue induced notable increases width certain Analysis demonstrated dynamic fluctuations Total Phenolic Content (TPC) Flavonoid (TFC) among While white red generally promoted higher TPC levels, unexpectedly exhibited highest levels at specific time points. Moreover, investigation into DPPH Radical Scavenging activity diverse temporal demonstrating exceptional early stages showing heightened later These findings underscore importance tailored regimes cultivated plants, thereby offering promising avenues sustainable agriculture food production practices.

Language: Английский

Citations

2
Physiological responses and variation in secondary metabolite content among Thai holy basil cultivars (Ocimum tenuiflorum L.) grown under controlled environmental conditions in a plant factory DOI Creative Commons
Panita Chutimanukul,

Hathairut Jindamol,

Akira Thongtip

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: Oct. 21, 2022

Holy basil ( Ocimum Tenuiflorum L.) is a widely used herb containing several bioactive compounds of interest for the food and pharmaceutical industries. Plant factories using artificial lighting (PFAL) modern agricultural system that offers opportunity to improve crop production stabilizes productivity in many herbal plants. However, little known about variation among holy varieties can be cultivated provide reasonable biomass PFAL. We therefore evaluated 10 Thai accessions two commercial cultivars PFAL (with hydroponic cultivation) categorize cultivar characteristics by investigating physiological responses secondary metabolite at plant flowering stage. Among varieties, net photosynthetic rate Pn ) was significantly highest OC059 OC081. The greatest growth measures were observed OC064. Antioxidant capacity also varied, with accumulation total phenolic (TPC), flavonoids, antioxidant activity DPPH assay OC064, terpenoid content OC194. major confirmed showing levels eugenol OC057, OC063, OC194, OC195 methyl OC072 α-humulene found OC059. PCA based on metabolites indicate OC064 clearly distinguished from other cultivars/accessions. These findings demonstrate across physiologic responses, capacity, insights lead identification suitable which most important step developing an efficient method producing high quality raw materials supplying foods

Language: Английский

Citations

11
Effect of gibberellic acid and potassium nitrate seed treatments on the emergence and seedling vigor of Amaranth and Cleome gynandra DOI Creative Commons
Linda Tapfumaneyi, Praxedis Dube, Sydney Mavengahama

et al.

Agrosystems Geosciences & Environment, Journal Year: 2023, Volume and Issue: 6(1)

Published: March 1, 2023

Abstract Seed dormancy is the major challenge in cultivation of Amaranth and Cleome gynandra . C. are affected by chemical, physical, physiological dormancy. The current study was conducted at Marondera University Agricultural Sciences Technology (MUAST) farm 2021 2022. effects gibberellic acid potassium nitrate seed treatments on emergence seedling vigour were evaluated. Seeds pre‐soaked (control 0, 100, 200, 300,400, 500 ppm) 0%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%) solutions for 24 h to have a factorial layout within randomized complete block design replicated three times. treated with showed significant difference while no difference. highest percentage vigor index recorded seeds ppm acid. maximum percentages (62.1%) (60.7%) obtained 2022 2021. least (46.4%) (45.3%) control (603) 2022, (562) lowest indices 341 270, which results that has potential reduce

Language: Английский

Citations

4