Characterization of the Optical and Thermal Properties of Cardiac Tissue as a Function of Temperature DOI
Leonardo Bianchi, Alessandro Bossi, Antonio Pifferi

et al.

2022 44th Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC), Journal Year: 2023, Volume and Issue: unknown, P. 1 - 4

Published: July 24, 2023

In this work, we devised the first characterization of optical and thermal properties ex vivo cardiac tissue as a function different selected temperatures, ranging from room temperature to hyperthermic ablative temperatures. The broadband (i.e., 650 nm 1100 nm) estimation properties, i.e., absorption coefficient (μa) reduced scattering $({\mu ^{\prime}}_s)$, was performed by means time-domain diffuse optics. Besides, measurement based on transient hot-wire technique, employing dual-needle probe estimate conductivity (k), diffusivity (α), volumetric heat capacity (Cv). Increasing led variations in spectral characteristics μa (e.g., redshift 780 peak, rise new peak at 840 nm, formation valley 900 nm). Moreover, an increase values ${\mu ^{\prime}}_s$ assessed raised for 800 25 °C ^{\prime}}_s = 9.8{\text{ c}}{{\text{m}}^{{\text{ - 1}}}}$, while 77 29.1{\text{ 1}}}}$). Concerning characterization, k almost constant interval. Conversely, α Cv were subjected decrease with temperature, respectively; thus, they registered 0.190 mm2/s 3.03 MJ/(m3•K) maximum investigated (79 °C), accordingly.Clinical Relevance- experimentally obtained are useful improve accuracy simulation-based tools therapy planning. Furthermore, measured can serve reference realization tissue-mimicking phantoms medical training testing instruments.

Language: Английский

Non-Fourier Bioheat Transfer Analysis in Brain Tissue During Interstitial Laser Ablation: Analysis of Multiple Influential Factors DOI Creative Commons
Sundeep Singh, Leonardo Bianchi, Sanzhar Korganbayev

et al.

Annals of Biomedical Engineering, Journal Year: 2024, Volume and Issue: 52(4), P. 967 - 981

Published: Jan. 18, 2024

This work presents the dual-phase lag-based non-Fourier bioheat transfer model of brain tissue subjected to interstitial laser ablation. The finite element method has been utilized predict tissue's temperature distributions and ablation volumes. A sensitivity analysis conducted quantify effect variations in input power, treatment time, fiber diameter, wavelength, phase lags. Notably, this work, temperature-dependent thermal properties have considered. developed validated by comparing obtained from numerical ex vivo during further extended settings incorporating blood perfusion effects. results systematic highlight importance considering tissue, behavior, microvascular effects computational models for accurate predictions outcomes ablation, thereby minimizing damage surrounding healthy tissue. parametric reported study would assist a more precise prediction distribution, thus allowing optimize dosage therapy brain.

Language: Английский

Citations

6
Temperature Dependence of Thermal Properties of Ex Vivo Porcine Heart and Lung in Hyperthermia and Ablative Temperature Ranges DOI Creative Commons
Leonardo Bianchi,

Martina Bontempi,

Sabrina De Simone

et al.

Annals of Biomedical Engineering, Journal Year: 2023, Volume and Issue: 51(6), P. 1181 - 1198

Published: Jan. 19, 2023

This work proposes the characterization of temperature dependence thermal properties heart and lung tissues from room up to > 90 °C. The diffusivity (α), conductivity (k), volumetric heat capacity (Cv) ex vivo porcine hearts deflated lungs were measured with a dual-needle sensor technique. α k associated tissue remained almost constant until ~ 70 80 °C, accordingly. Above more substantial variation in these was registered: at 94 respectively experienced 2.3- 1.5- fold increase compared their nominal values, showing average values 0.346 mm2/s 0.828 W/(m·K), Conversely, Cv 55 °C decreased afterward (e.g., = 2.42 MJ/(m3·K) °C). Concerning tissue, both its characterized by an exponential temperature, marked increment supraphysiological ablative temperatures (at 91 equal 2.120 2.721 respectively, i.e., 13.7- 13.1-fold higher baseline values). Regression analysis performed attain best-fit curves interpolating data, thus providing models investigated properties. These can be useful for increasing accuracy simulation-based preplanning frameworks interventional procedures, realization tissue-mimicking materials.

Language: Английский

Citations

12
Optical signatures of thermal damage on ex-vivo brain, lung and heart tissues using time-domain diffuse optical spectroscopy DOI Creative Commons
Alessandro Bossi, Leonardo Bianchi, Paola Saccomandi

et al.

Biomedical Optics Express, Journal Year: 2024, Volume and Issue: 15(4), P. 2481 - 2481

Published: Feb. 23, 2024

Thermal therapies treat tumors by means of heat, greatly reducing pain, post-operation complications, and cost as compared to traditional methods. Yet, effective tools avoid under- or over-treatment are mostly needed, guide surgeons in laparoscopic interventions. In this work, we investigated the temperature-dependent optical signatures ex-vivo calf brain, lung, heart tissues based on reduced scattering absorption coefficients near-infrared spectral range (657 1107 nm). These spectra were measured time domain diffuse optics, applying a step-like spatially homogeneous thermal treatment at 43 °C, 60 80 °C. We found three main increases spectra, possibly due denaturation collagen, myosin, proteins' secondary structure. After 75 rise two new peaks 770 830 nm formation chromophore, related hemoglobin myoglobin. This research marks significant step forward controlling with techniques identifying several key markers damage. could enhance ability monitor adjust real-time, promising improved outcomes tumor therapy.

Language: Английский

Citations

4
Thermal effects and biological response of breast and pancreatic cancer cells undergoing gold nanorod-assisted photothermal therapy DOI Creative Commons
Leonardo Bianchi, Sara Baroni, Gabriela Paroni

et al.

Journal of Photochemistry and Photobiology B Biology, Journal Year: 2024, Volume and Issue: 259, P. 112993 - 112993

Published: July 20, 2024

Language: Английский

Citations

3
Fiber Bragg Grating Sensors-Based Assessment of Laser Ablation on Pancreas at 808 and 1064 nm Using a Diffusing Applicator: Experimental and Numerical Study DOI Creative Commons
Pouya Namakshenas, Leonardo Bianchi, Paola Saccomandi

et al.

IEEE Sensors Journal, Journal Year: 2023, Volume and Issue: 23(16), P. 18267 - 18275

Published: July 11, 2023

Laser ablation (LA) holds great promise for the localized treatment of pancreatic tumors. However, there are still many technical aspects that require improvements before largely applying this technique in clinical practice. This work shed light on following subjects: thermal response pancreas to LA using a diffusing applicator and delivering different laser wavelengths (i.e., 808 nm 1064 nm); development pancreas-specific numerical model with temperature-dependent tissue properties based non-Fourier heat conduction predicting temperature distribution. To experimentally study tune parameters, 80 Fiber Bragg Grating (FBG) sensors, embedded arrays millimetric spatial resolution, were implanted ex vivo undergoing obtain real-time two-dimensional Based measured by FBG results show wavelength affects response: at 2 mm from applicator, resulted thermally affected area 5-15% larger than nm, whereas no notable difference was observed 6 mm. The distribution laser-induced lesion obtained found closely match experimental when phase lag flux vector five times gradient.

Language: Английский

Citations

9
Optimization of laser dosimetry based on patient-specific anatomical models for the ablation of pancreatic ductal adenocarcinoma tumor DOI Creative Commons
Pouya Namakshenas, Francesco Maria Di Matteo, Leonardo Bianchi

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: July 8, 2023

Laser-induced thermotherapy has shown promising potential for the treatment of unresectable primary pancreatic ductal adenocarcinoma tumors. Nevertheless, heterogeneous tumor environment and complex thermal interaction phenomena that are established under hyperthermic conditions can lead to under/over estimation laser efficacy. Using numerical modeling, this paper presents an optimized setting Nd:YAG delivered by a bare optical fiber (300 µm in diameter) at 1064 nm working continuous mode within power range 2-10 W. For analysis, patient-specific 3D models were used, consisting tumors different portions pancreas. The time ablating completely producing toxic effects on possible residual cells beyond margins found be 5 W 550 s, 7 8 s tail, body, head tumors, respectively. Based results, during irradiation doses, injury was not evident either 15 mm lateral distances from or nearby healthy organs. present computational-based predictions also line with previous ex vivo studies, hence, they assist therapeutic outcome ablation neoplasms prior clinical trials.

Language: Английский

Citations

7
Measurement of Thermal Conductivity and Thermal Diffusivity of Porcine and Bovine Kidney Tissues at Supraphysiological Temperatures up to 93 °C DOI Creative Commons
Leonardo Bianchi,

Silvia Fiorentini,

Sara Gianella

et al.

Sensors, Journal Year: 2023, Volume and Issue: 23(15), P. 6865 - 6865

Published: Aug. 2, 2023

This experimental study aimed to characterize the thermal properties of ex vivo porcine and bovine kidney tissues in steady-state heat transfer conditions a wider interval (23.2–92.8 °C) compared previous investigations limited 45 °C. Thermal properties, namely conductivity (k) diffusivity (α), were measured temperature-controlled environment using dual-needle probe connected commercial property analyzer, transient hot-wire technique. The estimation measurement uncertainty was performed along with assessment regression models describing trend quantities as function temperature be used simulations involving tissue. A direct comparison same tissue from two different species, i.e., tissues, technique, conducted provide indications on possible inter-species variabilities k α at selected temperatures. Exponential fitting curves interpolate values for both α. results show that remained rather constant room up onset water evaporation, more marked increase observed afterward. Indeed, highest investigated temperatures, 90.0–92.8 °C, average subject 1.2- 1.3-fold increases, their nominal temperature, tissue, respectively. Moreover, 1.4- 1.2-fold increases α, baseline values, No statistically significant differences found between temperatures despite anatomical structural differences. provided quantitative best-fit can enhance accuracy prediction capability numerical therapies. Furthermore, this may insights into refinement protocols realization tissue-mimicking phantoms choice bioheat studies laboratories.

Language: Английский

Citations

6
Müller matrix polarimetry for pancreatic tissue characterization DOI Creative Commons
Paulo Sampaio,

Maria Lopez-Antuña,

Federico Storni

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Sept. 29, 2023

Polarimetry is an optical characterization technique capable of analyzing the polarization state light reflected by materials and biological samples. In this study, we investigate potential Müller matrix polarimetry (MMP) to analyze fresh pancreatic tissue Due its highly heterogeneous appearance, type differentiation a complex task. Furthermore, challenging location in body makes creating direct imaging difficult. However, accurate reliable methods for diagnosing diseases are critical improving patient outcomes. To end, measured matrices ex-vivo unfixed human leverage feature-learning capabilities machine-learning model derive optimized data representation that minimizes normal-abnormal classification error. We show experimentally our approach accurately differentiates between normal abnormal tissue. This is, knowledge, first study use combined with from raw readings purpose.

Language: Английский

Citations

6
Extreme transport of light in spheroids of tumor cells DOI Creative Commons
Davide Pierangeli, Giordano Perini, Valentina Palmieri

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Aug. 3, 2023

Extreme waves are intense and unexpected wavepackets ubiquitous in complex systems. In optics, these rogue promising as robust noise-resistant beams for probing manipulating the underlying material. Localizing large optical power is crucial especially biomedical systems, where, however, extremely have not yet been observed. We here discover that tumor-cell spheroids manifest when illuminated by randomly modulated laser beams. The intensity of light transmitted through bio-printed three-dimensional tumor models follows a signature Weibull statistical distribution, where extreme events correspond to spatially-localized modes propagating within cell network. Experiments varying input beam size indicate nonlinear origin. show filaments form high-transmission channels with enhanced transmission. They deliver spheroid, can be exploited achieve local temperature increase controlled wave shape. Our findings shed on propagation biological aggregates demonstrate how event formation allows concentration deep tissues, paving way using applications, such light-activated therapies.

Language: Английский

Citations

5
Biophotonics as a new application in optical technology: A bibliometric analysis DOI Creative Commons
Lam Weng Siew, Lam Weng Hoe, Pei Fun Lee

et al.

Heliyon, Journal Year: 2023, Volume and Issue: 9(12), P. e23011 - e23011

Published: Nov. 29, 2023

Biophotonics procures wide practicability in life sciences and medicines. The contribution of biophotonics is well recognized various Nobel Prizes. Therefore, this paper aims to conduct a bibliometric analysis publications. scientific database used the Web Science database. Harzing's Publish or Perish VOSviewer are tools analysis. This study found an increasing trend number publications recent years as peaked at 347 2020. Most documents articles (3361 publications) proceeding papers (1632 publications). top three subject areas Optics (3206 publications), Engineering (1706 Radiology, Nuclear Medicine, Medical Imaging (1346 United States has highest (2041 citation impact (38.07 citations per publication; h-index: 125). publication titles Proceedings SPIE (920 Journal Biomedical (599 Society Photo Optical Instrumentation Engineers (245 potential for future research include overcome optical penetration depth issue develop publicly available biosensors detection common diseases.

Language: Английский

Citations

4